A study of the chikungunya virus in humans in Turkey

A study of the chikungunya virus in humans in Turkey

Background/aim: The chikungunya virus (CHIKV) is a mosquito-borne disease and has recently been causing explosive outbreaks. The CHIKV has spread throughout all continents. Although the first chikungunya case imported from India to Turkey was reported in 2012, there is no detailed epidemiologic study in Turkey yet. The aim of this study was to investigate the seroprevalence of the CHIKV in Turkey. Materials and methods: ELISA was used to screen 500 random serum samples of healthy people collected from Kırıkkale, which is located in central Anatolia in Turkey. The results were verified by indirect immunofluorescence test (IIFT). Results: The results showed that 0.4% samples were positive for CHIKV. In the verification study with IIFT, CHIKV IgG type antibodies were defined as negative. To the best of our knowledge, this is the first serological study on the CHIKV in Turkey. Conclusion: Further studies are needed to elucidate the epidemiological situation in patients that have fever and arthritis.

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  • 1. Vega-Rúa A, Lourenço-de-Oliveira R, Mousson L, Vazeille M, Fuchs S, Yébakima A, Gustave J, Girod R, Dusfour I, LeparcGoffart I et al. Chikungunya virus transmission potential by local Aedes mosquitoes in the Americas and Europe. PLoS Negl Trop Dis 2015; 9: e0003780. doi: 10.1371/journal. pntd.0003780.
  • 2. Petitdemange C, Wauquier N, Vieillard V. Control of immunopathology during chikungunya virus infection. J Allergy Clin Immunol 2015; 135: 846-855.
  • 3. Ross RW. The Newala epidemic. III. The virus: isolation, pathogenic properties and relationship to the epidemic. J Hyg (Lond) 1956; 54: 177-191.
  • 4. CDC; http://www.cdc.gov/chikungunya/geo/index.html.
  • 5. Yağcı Çağlayık D, Uyar Y, Korukluoğlu G, Ertek M, Unal S. An imported Chikungunya fever case from New Delhi, India to Ankara, Turkey: the first imported case of Turkey and review of the literature. Mikrobiyol Bul 2012; 46: 122-128.
  • 6. Thiberville SD, Boisson V, Gaudart J, Simon F, Flahault A, de Lamballerie X. Chikungunya fever: a clinical and virological investigation of outpatients on Reunion Island, South-West Indian Ocean. PLoS Negl Trop Dis 2013; 7: e2004. doi: 10.1371/ journal.pntd.0002004.
  • 7. Hasebe F, Parquet MC, Pandey BD, Mathenge EG, Morita K, Balasubramaniam V, Saat Z, Yusop A, Sinniah M, Natkunam S et al. Combined detection and genotyping of Chikungunya virus by a specific reverse transcription-polymerase chain reaction. J Med Virol 2002; 67: 370-374.
  • 8. Santhosh SR, Parida MM, Dash PK, Pateriya A, Pattnaik B, Pradhan HK, Tripathi NK, Ambuj S, Gupta N, Saxena P et al. Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus. J Clin Virol 2007; 39: 188-193.
  • 9. Ho PS, Ng MM, Chu JJ. Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection. Virol J 2010; 7: 13.
  • 10. Litzba N, Schuffenecker I, Zeller H, Drosten C, Emmerich P, Charrel R, Kreher P, Niedrig M. Evaluation of the first commercial chikungunya virus indirect immunofluorescence test. J Virol Methods 2008; 149: 175-179.
  • 11. Yap G, Pok KY, Lai YL, Hapuarachchi HC, Chow A, Leo YS, Tan LK, Ng LC. Evaluation of Chikungunya diagnostic assays: differences in sensitivity of serology assays in two independent outbreaks. PLoS Negl Trop Dis 2010; 4: e753. doi: 10.1371/ journal.pntd.0000753.
  • 12. Rianthavorn P, Wuttirattanakowit N, Prianantathavorn K, Limpaphayom N, Theamboonlers A, Poovorawan Y. Evaluation of a rapid assay for detection of IgM antibodies to chikungunya. Southeast Asian J Trop Med Public Health 2010; 41: 92-96.
  • 13. Okabayashi T, Sasaki T, Masrinoul P, Chantawat N, Yoksan S, Nitatpattana N, Chusri S, Morales Vargas RE, Grandadam M, Brey PT et al. Detection of chikungunya virus antigen by a novel rapid immunochromatographic test. J Clin Microbiol 2015; 53: 382-388.
  • 14. Rolph MS, Zaid A, Mahalingam S. Salivary transmission of the chikungunya arbovirus. Trends Microbiol 2016; 24: 86-87.
  • 15. Burt FJ, Rolph MS, Rulli NE, Mahalingam S, Heise MT. Chikungunya: a re-emerging virus. Lancet 2012; 379: 662-671.
  • 16. Rossini G, Gaibani P, Vocale C, Finarelli AC, Landini MP. Increased number of cases of Chikungunya virus (CHIKV) infection imported from the Caribbean and Central America to northern Italy, 2014. Epidemiol Infect 2016; 1-5.
  • 17. Alten B, Bellini R, Caglar SS, Simsek FM, Kaynas S. Species composition and seasonal dynamics of mosquitoes in the Belek region of Turkey. J Vector Ecol 2000; 25: 146-154.
  • 18. Akiner MM,  Demirci B, Babuadze G,  Robert V, Schaffner F. Spread of the invasive mosquitoes  Aedes aegypti  and  Aedes albopictus in the Black Sea Region increases risk of chikungunya, dengue, and zika outbreaks in Europe. PLoS Negl Trop Dis 2016; 10: doi: 10.3201/eid2012.141269.
  • 19. European Centre for Disease Prevention and Control (ECDC). Mosquito maps. http://ecdc.europa.eu/en/healthtopics/ vectors/vector-maps/Pages/VBORNET_maps.aspx (accession date: 20.02.2017).
  • 20. Prat CM, Flusin O, Panella A, Tenebray B, Lanciotti R, LeparcGoffart I. Evaluation of commercially available serologic diagnostic tests for chikungunya virus. Emerg Infect Dis 2014; 20: 2129-2132.