Azadeh RASOOLI, Elham GHAFARI, Hamed SAEDI, Saba MIRI

Expression changes of CD177 and MPO as novel biomarkers in lung tissue of CLP model rats

Background/aim: Sepsis is an unregulated systemic response to microbial invasion that can lead to multiple organ failure. This study aims at investigating the relationships among myeloperoxidase (MPO) and CD177 in major organ systems including whole blood, liver, and lung tissues in septic rats. Materials and methods: Sepsis was induced by cecal ligation and puncture (CLP) in female Wistar rats. Whole blood, liver, and lung samples were obtained from rats of 3 groups (n = 10 for each group, n total = 30: control as a wild-type group, laparotomy group (LAP), and CLP). Gene expression of MPO and CD177 in targeted tissues was determined by real-time PCR after CLP. MPO activity was also determined by ELISA method for the result validation of the real-time PCR. Results: Expression levels of MPO increased significantly in all targeted organs in the CLP group, while CD177 expression was upregulated only in lung tissue in response to sepsis (P < 0.05). The results obtained with ELISA analysis also show that MPO level was significantly increased in all the targeted tissues in the CLP group (P < 0.05). Conclusion: A high level of MPO as an inflammatory enzyme can be a potentially novel biomarker for sepsis in all organs. On the other hand, CD177 may be a marker in lung tissue.

Keywords:

Sepsis, gene expression, myeloperoxidase, CD177,

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