Investigation of the effect of β-lactam antibiotics and serum on growth and gene expression in Escherichia coli strain JJ1886
Background/aim:
ß-Lactamase-producing
Escherichia
coli
strain JJ1886 is an epidemic clone with high virulence properties. Because
this
strain can survive in the bloodstream, we aimed here to understand how ß-lactam antibiotics and human serum affect the growth
and gene expression of this bacterium.
Materials and methods:
We report the time-dependent growth effect of normal human serum and heat-inactivated serum, together
with ß-lactam antibiotics (including cefotaxime, ceftazidime, and carbenicillin), for
E. coli
strain JJ1886. Relative gene expression of
ß-lactamase-related genes (encoding the ß-lactamase regulator, CTX-M-15, and peptidoglycan glycosyltransferase) and serum survival-
associated genes (encoding lipoprotein NlpI, murein lipoprotein, lipopolysaccharide core heptose (I) kinase, lipopolysaccharide
biosynthesis protein, capsule synthesis protein, and phosphate transport system) were investigated by RT-qPCR.
Results:
Cells proliferated during the exponential growth phase when the bacterium was treated with human serum. However,
cefotaxime and ceftazidime together with serum had a bactericidal effect at each of the tested time points. Downregulation was observed
in gene-encoding lipoprotein NlpI as a result of treatment with carbenicillin.
Conclusion:
Serum plus cefotaxime or ceftazidime had bactericidal activities. When the bacterium was treated with human serum and
ß-lactam antibiotics, there were no significant changes in relative gene expression, except for the
nlpI
gene.
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