Background/aim: A wide variety of neurological and psychiatric disorders have been shown to be closely related to changes in hippocampal volume (HV). It appears that hippocampal volumetry will be an indispensable part of clinical practice for a number of neuropsychiatric disorders in the near future. The aim of this study was to establish a normative data set for HV according to age and sex in the general population.Materials and methods: Hippocampal magnetic resonance imaging scans of 302 healthy volunteers were obtained using a 1.5 T unit with a 20-channel head coil. The hippocampal volumetric assessment was conducted using the volBrain fully automated segmentation algorithm on coronal oblique T1-weighted magnetization prepared rapid gradient-echo (MP-RAGE) images obtained perpendicular to the long axis of the hippocampus. The mean values of HV of groups according to age and sex were calculated. The associations between HV and age and sex were analyzed. Results: The mean HV of the study group was found to be 3.81 ± 0.46 cm3. We found that the mean HV of males (3.94 ± 0.49 cm3) was significantly higher than that of females (3.74 ± 0.42 cm3), and the mean right HV (3.86 ± 0.48 cm3) was significantly higher than that of the left HV (3.78 ± 0.49 cm3) (P = 0.001). Among both females and males, there were statistically significant but poor negative correlations between age and volumetric measurements of both the right and the left hippocampi (P