M. Radha Krishna REDDY, S.A MASTAN

Wohlfahrtiimonas chitiniclastica fulminant sepsis in Pangasius sutchi-first report

This communication provides an insight in to the emerging of new infection fulminant sepsis in Pangasius sutchi and aimed to screen prime pathogens involved in disease. The pathogen was isolated from diseased P. sutchi characterized by morphological, biochemical and molecular approach, which includes 16s r RNA gene sequencing. PCR amplified 16s RNA was separated using agarose gel electrophoresis, eluted product was sequenced and blast analysis was carried out to identify the pathogen. Wohlfahrtiimonas chitiniclastica with LD50 dose 108.35 has been initiated re-infection in experimentally infected Pangasius fingerlings. This study provides the evidence of newly emerged Wohlfahrtiimonas chitiniclastica which was true causative agent of fulminant sepsis in Pangasius. There was no track record of Wohlfahrtiimonas chitiniclastica fulminant sepsis infection in P. sutchi till date around globe. To the best of knowledge, this is the first report of Wohlfahrtiimonas chitiniclastica infection in Pangasius sutchi.

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Almuzara, M.N., Palombarani, S., Tuduri, A., Figueroa, S., Gianecini, A., Sabater, L., Ramirez, M.S. and Vay, C.A. 2011. First case of fulminant sepsis due to Wohlfahrtiimonas chitiniclastica. Journal of clinical microbiology, 49(6): 2333-2335. doi: 10.1128/JCM.00001-11

APHA, 1998. Standard method for the examination of water and waste water 20th Edition, Washington D.C: 12-13.

Cao, X.M., Chen, T., Xu, L.Z., Yao, L.S., Qi, J., Zhang, X.L. and Xu, B.L. 2013. Complete genome sequence of Wohlfahrtiimonas chitiniclastica strain SH04, isolated from Chrysomya megacephala collected from Pudong International Airport in China. Genome announcements, 1(2):1-2. doi: 10.1128/genomeA.00119-13

Dung, T.T., Ngoc, T.N.N., Thinh, N.Q., Thy, D.T.M. and Tuan, N.A., Shinn, A. and Crumlish, M. 2008. Common Diseases Of Pangasius Catfish Farmed In Vietnam. Global Aquaculture the Advocate, 11(4): 77-78. http://pdf.gaalliance.org/pdf/GAA-Dung- July08.pdf

Farkas, R., Hall, M.J.R and Kelemen, F. 1997. Wound myiasis of sheep in Hungary. Vet. Parasitol., 69: 133- 144. doi: 10.1016/S0304-4017(96)01110-7.

Hall. M.J.R. and Wall, R. 1995. Myiasis of humans and domestic animals. Adv. Parasitol., 35: 257-334. doi: 10.1016/S0065-308X(08)60073-1

Juteau, P., Tremblay, D., Ould-Moulaye, C.B., Bisaillon, J.G., Beaudet, R. 2004. Swine waste treatment by self-heating aerobic thermophilic bioreactors. Water Res., 38: 539546. doi: 10.1016/j.watres.2003.11.001.

Nedelchev, N. K 1988. Distribution and causes of Myiasis among farm animals. Veterinarna sbrika 86, 33-35.

Rebaudet, S., Genot, S., Renvoise, A., Fournier, P.E. and Stein, A. 2009. Wohlfahrtiimonas chitiniclastica bacteremia in homeless woman. Emerging Infectious Diseases, 15(6): 985-987. doi: 10.3201/eid1506.080232

Reed, L.J. and Muench, H. 1938. A simple method of estimating fifty per cent endpoints. American Journal of Epidemiology, 27(3): 493-497.

Stewart, C.N. and Via, L.E. 1993. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques, 14(5): 748-750.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2004. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10): 2731-2739. doi: 10.1093/molbev/msr121

Tóth, E.M., Schumann, P., Borsodi, A.K., Kéki, Z., Kovács, A.L. and Márialigeti, K. 2008. Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae). International Journal of Systematic and Evolutionary Microbiology, 58(4): 976-981. doi: 10.1099/ijs.0.65324-0

Tóth, E., Farkas, R., Marialigeti, K. and Mokhtar, I.S. 1998. Bacteriological investigations on wound myiasis of sheep caused by Wohlfahrtia magnifica (Diptera: Sarcophagidae). Acta Veterinaria Hungarica, 46(2): 219-229.