Herpandi, Nurul HUDA, Rosma AHMAD, Wan Nadiah Wan ABDULLAH

Protein Quality of Hydrolyzed Dark Muscle Protein of Skipjack Tuna (Katsuwonus pelamis)

Protein quality of protein hydrolysates prepared from dark flesh skipjack tuna, hydrolyzed using 2.04 % Alcalase® (FPH-A) and 3 % Protamex® (FPH-P) were evaluated and compared with commercial fish protein hydrolysate (FPH-C). The protein quality was determined using the analysis of molecular weight, amino acid profile, amino acid score/chemical score, protein digestibility correction amino acid score, essential amino acid index, protein efficiency ratio, biological value and its in-vitro digestibility. The results conclude that the FPH-C possessed wider distribution of molecular weight ranged from 237 Da to 11770 Da (mean 3231), in which the largest proportion were at <1000 Da (20%) and >5000 Da (20%). FPH-P possessed narrower distribution range (1210-7246 Da) which 50% of the protein was at 2000-3000 Da. On the other hand, FPH-A's was at range of 730-6440 Da dominated with low molecular weight protein (40% of 1000-2000 Da). Skipjack tuna hydrolysate prepared using Alcalase® showed higher aminoacids concentration, chemical score of protein, protein efficiency ratio (PER), biological value and protein digestibility correction amino acid score (PDCAAS) compared to hydrolysate repared using protamex and commercially sold hydrolysate tested.

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Abdul-Hamid, A., Bakar, J., & Bee, G. H. (2002). Nutritional quality of spray dried protein hydrolysate from Black Tilapia (Oreochromis mossambicus). Food Chemistry, 78(1), 69-74. DOI: 10.1016/S0308-8146(01)00380-6

Alsmeyer, R. H., Cunningham, A. E., & Happich, M. L. (1974). Equations predict PER from amino acid analysis. Food Technology, 28(7), 34-38.

AOAC. (2000). Official Methods of Analysis of the Association of Agricultural Chemists. 17th edition. AOAC. Washington, DC, USA.

Babji, A.S., & Letchumanan S.K. (1989). Measuring protein digestibility using an enzymatic in vitro technique. Proceedings Nutrition Society Malaysia, 4, I53-58.

Bhaskar, N., Benila, T., Radha, C., & Lalitha, R. G. (2008). Optimization of enzymatic hydrolysis of visceral waste proteins of Catla (Catla catla) for preparing protein hydrolysate using a commercial protease. Bioresource Technology, 99(2), 335-343.DOI: 10.1016/j.biortech.2006.12.015

Borseau, P., Vandanjon, L., Jaouen, P., ChaplainDerouiniot, M., Massé, A., et al., (2009). Fractionation of fish protein hydrolysates by ultrafiltration and nanofiltration: impact on peptidic populations. Desalination, 244(1-3), 303-320.DOI: 10.1016/j.desal.2008.05.026

Cao, W., Zhang, C., Hong, P., & Ji, H. (2008). Response surface methodology for autolysis parameters optimization of shrimp head and amino acids released during autolysis. Food Chemistry, 109(1), 176- 183.DOI: 10.1016/j.foodchem.2007.11.080

Chalamaiah, M., Narsing Rao, G., & Jyothirmayi, T. (2010). Protein hydrolysates from meriga (Cirrhinus mrigala) egg and evaluation of their functional properties. Food Chemistry, 120, 652-657.DOI: 10.1016/j.foodchem.2009.10.057

Chapot-Chartier, M.P., & Mistou, M.Y. (2004). PepC aminopeptidase of lactic acid bacteria. In "Handbook of Proteolytic Enzymes" (A.J. Barrett, N.D. Rawlings, and J.F. Woessner, Eds.), pp. 1202-1204, Elsevier, London.

Doucet, D., Otter, D. E., Gauthier, S. F., & Foegeding, E. A. (2003). Enzyme-induced gelation of extensively hydrolyzed whey proteins by Alcalase: Peptide identification and determination of enzyme specificity. Journal of Agriculture and Food Chemistry, 51, 6300-6308. DOI: 10.1021/jf026242v

Dudley, E.G. and Steele, J.L. (2004). Dipeptidase DA. In Handbook of Proteolytic Enzymes (A.J. Barrett, N.D. Rawlings, and J.F. Woessner, Eds.), pp. 2052-2053, Elsevier, London.

El, S. N., & Kavas, S. N. (1996). Determination of protein quality of rainbow trout (Salmo irideus) by in vitro protein digestibility - corrected amino acid score (PDCAAS). Food Chemistry, 55(3), 221-223. DOI: 10.1016/0308-8146(95)00111-5

Elena, S., Mahassine, A., Rosa, O., Evangélica, F., Casilda, N., Juana, F., et al. (2011). Quality characteristics of dark muscle from yellowfin tuna Thunnus albacores to its potential application in food industry. Food and Nutrition Sciences, 2, 22-30. DOI:10.4236/fns.2011.21003

FAO. (2010). Fishery Statistical Collections: Global Tuna Catches by stock 1950-2007. http://www.fao.org/fishery/statistics/tunacatches/query/en. Accessed February 24, 2010.

FAO/WHO (Food and Agriculture Organization/World Health Organization) 1990. Protein quality evaluation. Report of a joint FAO/WHO expert consultation held in Bethesda, Md. 1989 Dec 4-8. Rome: FAO/WHO.

FAO/WHO. (1965). Protein requirements. WHO Technical report series, No. 301, WHO, Geneva.

Fernandez, G., & Venkatrammann, J. (1993). Role of omega-3 fatty acids in health and disease. Nutrition Research, 1(Suppl. 13), 19-45. DOI: 10.1016/S0271- 5317(05)80282-9

Foh, M. B. K., Amadou, I., Foh, B. M., Kamara, M. T., & Xia, W. (2010). Functionality and antioxidant properties of tilapia (Oreochromis niloticus) as influenced by the degree of hydrolysis. International Journal of Molecular Sciences, 11(4), 1851-1869. DOI: 10.3390/ijms11041851

Guerard, F., Dufosse, L., De La Broise, D., & Binet, A. (2001). Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacares) wastes using Alcalase. Journal of Molecular Catalysis B: Enzymatic, 11(4), 1051-1059. DOI: 10.1016/S1381- 1177(00)00031-X

Hale, M. B., & Bauersfeld, P. E. (1972). Preparation of a Menhaden hydrolysate for possible use in a milk replacer. Marine Fisheries Review, 1320, 14-17.

Herpandi, N. H., Rosma, A., & Wan Nadiah, W. A. (2011). The Tuna Fishing Industry: A New Outlook on Fish Protein Hydrolysates. Comprehensive Reviews in Food Science and Food Safety, 10(4), 195-207. DOI: 10.1111/j.1541-4337.2011.00155.x

Herpandi, Huda, N., Rosma, A., & Wan Nadiah, W. A. (2012). Degree of hydrolysis and free tryptophan content of Skipjack Tuna (Katsuwonus pelamis) protein hydrolysates produced with different type of industrial proteases International Food Research Journal, 19(3), 863-867.

Herpandi, Huda, N., Rosma, A., & Wan Nadiah, W. A. (2013). Optimization of Enzymatic Hydrolysis of Skipjack Tuna (Katsuwonus pelamis) Dark Flesh Using Alcalase®: A Response Surface Approach. Journal of Fisheries and Aquatic Science, 8:494-505. DOI: 10.3923/jfas.2013.494.505

Ismail, H. M. (2005). The role of omega-3 fatty acids in cardiac protection: an overview. Frontiers in Bioscience, 10, 1079-1088. DOI: 10.2741/1601

Lee, Y. B., Elliott, J. G., Rickansrud, D. A., & Hagberg, E. Y. C. (1978). Predicting protein efficiency ratio by the chemical determination of connective tissue content in meat. Journal of Food Science, 43(5), 1359-1362. DOI: 10.1111/j.1365-2621.1978.tb02490.x

McGuire, M. & Beerman, K. A. (2007). Nutritional sciences: From fundamentals to food. Thomson Wadsworth, Belmont.

Nakajima, K., Yumiko, Y., & Mariko, O. (2009). Comparison of ACE inhibitory and DPPH radical scavenging activities of fish muscle hydrolysates. Food Chemistry, 114, 844-851. DOI: 10.1016/j.foodchem.2008.10.083

Niittynen, L., Nurminen, M. L., Korpela, R., & Vapaatalo, H. (1999). Role of arginine, taurine 4 and homocysteine in cardiovascular diseases. Annals of Medicine, 31(5), 318-326. DOI: 10.3109/07853899908995898

Nishioka, M., Tanioka, Y., Miyamoto., E., Enomoto, T., & Watanabe, F. (2007). TLC analysis of a Corrinoid compound from dark muscle of the yellowfin tuna (Thunnus albacores). Journal of Liquid Chromatography & Related Technologies, 30(13-16), 2245-2252. DOI: 10.1080/10826070701451605

NRC (1993). National Research Council. National Academy of Sciences, Nutrient Requirements of Fish, Washington.

Oser, B. L. (1951). Method for integrating essential amino acid content in the nutritional evaluation of protein. Journal of the American Dietetic Association, 27(5), 396.

Oser, B.L. (1959). An integrated essential amino acid index for predicting the biological value of proteins. In Protein and Amino Acid Nutrition (A. A. Albanese. Ed.). pp 281-295. Academic Press, New York.

Ovissipour, M., Taghiof, M., Motamedzadegan, A., Rasco, B., & Molla, A. E. (2009a). Optimization of enzymatic hydrolysis of visceral waste proteins of beluga sturgeon Huso huso using Alcalase. International Aquatic Research,1(1), 31-38.

Ovissipour, M., Abedian, A., Motamedzadegan, A., Rasco, B., Safari, R., & Shahiri, H. (2009b). The effect of enzymatic hydrolysis time and temperature on the properties of protein hydrolysates from Persian sturgeon (Acipenser persicus) viscera. Food Chemistry, 115(1), 238-242. DOI: 10.1016/j.foodchem.2008.12.013

Ovissipour, M., Benjakul, S., Safari, R., & Motamedzadegan, A. (2010). Fish protein hydrolysates production from yellowfin tuna (Thunnus albacares) head using Alcalase and Protamex. International Aquatic Research, 2, 87-95.

Ovissipour, M., Kenari, A. A., Motamedzadegan, A., Rasco, B., & Nazari, R. M. (2011). Optimization of protein recovery during hydrolysis of yellowfin tuna (Thunnus albacares) visceral proteins. Journal of Aquatic Food Product Technology, 20(2), 148-159. DOI: 10.1080/10498850.2010.548910

Peña-Ramos, E. A., XXiong, Y. L., & Artega, G. E. (2004). Fractionation and characterization for antioxidant activity of hydrolysed whey protein. Journal of the Science of Food and Agriculture, 84, 1908-1918. DOI: 10.1002/jsfa.1886

Pihlanto, A. (2006). Review: Antioxidative peptides derived from milk proteins. International Dairy Journala, 16, 1306-1314. DOI: 10.1016/j.idairyj.2006.06.005

Sathivel, S., Bechtel, P. J., Babbitt, J., Smiley, S., Crapo, C., & Reppond, K. D. (2003). Biochemical and functional properties of herring (Clupea harengus) by product hydrolyssates. Journal of Food Science, 68, 2196- 2200. DOI: 10.1111/j.1365-2621.2003.tb05746.x

Sikorski, Z. E. (1994). Charakterystyka bialek glownych surowcow zywnos´ ciowych. In Chemiczne I funkcjonalne wlas´ciwos´ci skladnikow zywnos´ci. WN-T, Warszawa.

Sindayikengera, S. (2006). Nutritional evaluation of caseins and whey proteins and their hydrolysates from Protamex. Journal of Zhejiang University Science B, 7(2), 90-98. DOI: 10.1631/jzus.2006.B0090

?li?yt?, R., Dauk?as, E., Falch, E., Storrø, I., & Rustad, T. (2005). Characteristics of protein fractions generated from cod (Gadus morhua) by-products. Process Biochemistry, 40, 2021-2033. DOI: 10.1016/j.procbio.2004.07.016

Sultanbawa, Y., & Aknes, A. 9 (2006). Tuna process waste: An unexploited resource. Infofish International, 3, 37- 40.

Usydus, Z., Joanna, S., & Adamczyk, M. (2009). Protein quality and amino acid profile of fish product available in Poland. Food Chemistry, 112, 139-145. DOI: 10.1016/j.foodchem.2008.05.050

Wergedahl, H., Liaset, B., Gudbrandsen, O. A., Lied, E., Espe, M., Muna, Z., et al. (2004). Fish protein hydrolisate reduces plasma total cholesterol, increases the propoertion of HDL cholesterol and lowers acylCoA: cholesterol acyltransferase activity in liver of Zucker rats. Journal of Nutrition, 134, 1320-1327.

WHO. (2007). Protein and Amino Acid Requirements in Human Nutrition. World Health Organization. pp.