ALİ ATASEVER, MURAT ARSLAN, Necdet SİRKECİOĞLU, Abdulkadir BAYIR, DENİZ EKİNCİ, MURAT ŞENTÜRK

Influence of different dietary lipids on the activity of metabolic enzymes in brown trout (salmo trutta)

Mevcut çalışmanın amacı farklı diyetsel yağ kaynaklarının kahverengi alabalıkta hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) gibi metabolik enzimler üzerine etkisini araştırmaktır. Tek farkları içerdikleri yağ kaynağı olan dört iso-nitrogenous ve iso-lipidik kazein-jelatin tabanlı yem formüle edilmiştir. Diyetsel yağ kaynakları olarak, ringa balığı yağı (MO diyeti, çoklu doymamış yağ asitleri bakımından zengin), fındık yağı (HO diyeti, başlıca oleik asit içeriyor), keten tohumu yağı/ soya yağı karışımı (1:1) (LO+SO diyeti, linoleik asit ve linolenik asit bakımından zengin) ve soya lesitini (LE diyeti, forfolipid yapısında ve çoğunlukla linoleik asit içeriyor) kullanılmıştır. Başlangıç ağırlıkları yaklaşık 1g olan balıklar, 50 litrelik cam akvaryumlara 30ar adet, üç tekerrürlü olarak konulmuştur. Balıklar deneysel yemlerle 6 hafta boyunca beslenmişlerdir. Altı haftalık deneme besleme denemesi sonunda, bütün gruplarda metabolik enzim aktiviteleri başlangıç değerlerine göre önemli artış göstermiştir (P

Kahverengi alabalıklarda (salmo trutta) farklı diyetsel yağ kaynaklarının bazı metabolik enzim aktiviteleri üzerine etkisi

The objective of this experiment was to test the effect of different dietary lipids in brown trout feeding on the activity of metabolic enzymes, such as hexokinase (HK), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD). Four iso-nitrogenous and iso-lipidic casein-gelatin based experimental diets with varying dietary sources were formulated. The sources of dietary lipid were menhaden oil (MO diet, rich in highly unsaturated fatty acids), hazelnut oil (HO diet, predominantly oleic acid), a blend of linseed oil and soybean oil (1:1) (LO+SO diet, linoleic and linolenic acids), and soybean lecithin (LE diet, phospholipids; mostly linoleic acid). Fish with an initial individual weight of ~1g were distributed randomly in three replicate 50-L glass aquaria with a total of 30 fish per tank. Each tank received one of the four diets referenced above for 6 weeks. In all cases, the activity of all metabolic enzymes significantly increased (P<0.05) in comparison to their activities in initial fish. All enzyme activities had their highest values in fish fed LO+SO and lowest in fish fed HO diet. It is very important to note that total polyunsaturated fatty acid (PUFA) content of the lipids is in the same order indicating that activity of these enzymes are elevated with increasing PUFA concentrations.

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