MUHAMMED ARABACI, HAŞMET ÇAĞIRGAN, MUSTAFA SARI

Induction of spawning in common carp (Cyprinus carpio) using LHRHa (D-Ser $(tBu)^6$, $Pro^9$-NEt -LHRH) combined with haloperidol: Effects of different treatment time and determination of latency period dependence on temperature

A single injection of 20$\mu$g/kg LHRHa $([D-Ser (tBu)^6, Pro^9-NEt]-LHRH)$ combined with 0.5 mg/kg of the dopamine receptor antagonist, Haloperidol (LHRHa+HAL) was used for induction of spawning in the common carp under routine hatchery conditions. This treatment caused successful ovulation. Latency in common carp injected with LHRHa+HAL was dependent on water temperature, and minimal latency was 14 h. When LHRHa+HAL was given at various hours of the day it resulted in identical spawning with the same latency. These results show that the predicting latency at a given temperature may provide successful hatchery management for fish farmers.

Anahtar Kelimeler:

sıcaklık, haloperidol, sazan balığı, yumurta bırakmak, Cyprinidae

PDF

___

Alvarino, J.M.R., Zanuy, S., Prat, F., Carrillo, M. and Mananos, F. 1992. Stimulation of ovulation and steroid secretion by LHRHa injection in the sea bass (Dicentrarchus labrax): effect of time of day. Aquaculture, 102: 177-186.

Anderson E., Borg, B., Leeuw R. D. 1989. Characterisation of gonadotropin-relasing hormone binding sites in the pituitary of the three stickleback (Gasterosteus aculeatus). Gen. and Comp. Endocrinology, 76: 41- 45.

Arabacı, M. 2000. Çipura (Sparus aurata) ve levrek (Dicentrarchus labrax) anaçlarında depo hormon kullanarak üremenin uyarılması, senkranizasyonu ve yumurta kalitesinin arttırılması üzerine bir çalışma. Doktora Tezi, İzmir: Ege Univ. Fen Bilimleri Enst., 87 s.

Bieniarz, H., Popek, W., Breton, H. and Epler, P. 1985. Daily changes in the gonadotropin levels and response of carp oocytes to hypophysial homogenate. Chronobiol. Int., 2: 93-101.

Chang, J.P., Peter, R.E., Nahorniak, C.S. and Sokolowska, M. 1984. Effects of catecholaminergic agonists and antagonists on serum gonadotropin concentrations and ovulation in goldfish: evidence for specificity of dopamine inhibition of gonadotropin secretion. Gen. Comp. Endocrinology, 55: 351-360.

Copeland, P.A., and Thomas, P. 1989. Control of gonadotropin release in the croaker (Micropogonias undulatus): evidence for lack of dopaminergic inhibition., Gen. and Comp. Endocrinology, 74: 474- 483.

Crim, L., W., Arnaud, R., St., Lavoie, M., and Labrie, F. 1988a. A study of LHRH receptors in the pituitary gland of the Winter flounder (Pseudopleuronectes americanus Walbaum). Gen. and Comp. Endocrinology, 69: 372-377.

Crim, LW., Nestor, J.J. and Wilson, C.E. 1988b. Studies of the biological activity of LHRH ana1ogs in the rainbow trout, landlocked salmon, and the winter flounder. Gen. Comp. Endocrinology., 71: 372-382.

Drori S., Ofir, M., Sivan, B.L., Yaron, Z. 1994. Spawning induction in common carp (Cyprinus carpio) using pituitary extract or GnRH superactive analogue combined with metoclopramide: analysis of hormone profile, progress of oocyte maturation and dependence on temperature. Aquaculture, 119: 393-407.

Glubokov, Al., Motloch, N.N. and Sedova, M.A. 1991. Effect of synthetic LHRH analogue and dopamine antagonists on the maturation of bream, Abramis brama L. Aquaculture, 95: 373-377.

Habibi R. H., Marchant, T. A., Carol S., Nahorniak, C. S., Loo H. V. D., Peter, R. E., Rivier J. E., Vale, W.W. 1989. Functional relationship between receptor binding and biological activity for analogues of mammalian and salmon gonadotropin-releasing hormones in the pituitary of goldfish (Carassius auratus). Biology of Reproduction, 40: 1152-1161.

Horvath, L. 1978. Relation between ovulation and water temperature by farmed cyprinids. Aquacult. Hung. (Szarvas), 1: 58-65.

Horvath, L. 1985. Egg development (oogenesis) in the common carp (Cyprinus carpio L.). J.F. Muir and R.J. Roberts (Eds.), Recent Advances in Aquaculture, Vol. 2. Croom Helm, London, 32-77.

Leeuw, R. De, Veer, C. Van’t, Smit-van Dijk, W., Goos, H.J. Th. and Oordt, P.G.W.J. Van. 1988. Binding affinity and biological activity of gonadotropin- releasing analogues in the African catfish, Clarius gariepinus. Aquaculture, 71: 119-131.

Levavi-Zermonsky, B. and Yaron, Z. 1986. Changes in gonadotropin and ovarian steroids associated with oocytes maturation during spawning induction in the carp. Gen. Comp. Endocrinology, 62: 89-98.

Lin, H.R., Kraak, G.V.D., Liang, J.Y., Peng, C., Li, G.Y., Lu, L.Z., Zhou, X.J., Chang, M.L., and Peter, RE. 1986. The effects of LHRH analogue and drugs which block the effects of dopamine on gonadotropin secretion and ovulation in fish cultured in China. R. Billard and J. Marcel (Eds.), Aquaculture of Cyprinids, INRA, Paris, 139-50.

Lin, H.R., Van Dcr Kraak, G., Zhou, X.J., Liang, J.Y., Peter, R.E., Rivier, J.E. and Vale, W.W. 1988. Effects of [D-Arg6, Trp7, Leu8, Pro9-NEt]-luteinizing hormone-releasing hormone (sGnRH-A) and [D- Ala6Pro9-NEt]-luteinizing hormone-releasing hormone (LHRHA), in combination with pimozide or domperidone, on gonadotropin release and ovulation in the Chinese bach and common carp. Gen. Comp. Endocrinology, 69: 31-40.

Omeljaniuk, R.J., Shih, S.H. and Peter, RE. 1987. ln-vivo evaluation of dopamine receptor-mediated inhibition of gonadotropin secretion from the pituitary gland of the goldfish, Carrassius auratus. J. Endocrinol., 114: 449-458.

Peter, R.E., Chang, J.P., Nahorniak, C.S., Omeljeniuk, R.J., Sokolowska, M., Shih, S.H. and Billard, R. 1986. Interactions of catecholamines and GnRH in regulation of gonadotropin secretion in teleost fish. Recent Prog. Horm. Res., 42: 513-548.

Peter, R.E., Lin, H.R. and Van Der Kraak, G. 1987. Drug/hormone induced breeding of Chinese teleosts. D.R. Idler, LW. Crim and J.M. Walsh (Eds.), Proceedings of the Third international Symposium on the Reproductive Physiology of Fish, St. John’s, Newfoundland, Canada, 2-7 August 1987, Memorial University of Newfoundland, Canada, 120-123.

Peter, R.E., Lin, H.R. and Van Der Kraak, G. 1988. Induced ovulation and spawning of cultured freshwater fish in China: advances in application of GnRH analogues and dopamine antagonists. Aquaculture, 74: 1-10.

Peter, R.E., Lin, H.R., Van Der Kraak, G. and Little, M. 1993. Releasing hormones, dopamine antagonists and induced spawning. J.F. Muir and R.J. Roberts (Eds.), Recent Advances in Aquaculture, Vol. IV, Blackwell, Oxford, 25-30.

Rothbard, S. 1981. Induced reproduction in cultivated cyprinids-the common carp and the group of Chinese carps. I. The technique of induction, spawning and hatching. Bamidgeh, 33: 103-121.

Rottmann, R.W. and Shireman, J.V. 1985. The use of synthetic LH-RH analogue to spawn Chinese carp. Aquacult. Fish. Manage, 1: 19-24.

Yaron, Z., Bogomolnaya, A. and Lcvavi, B. 1984. A calibrated carp pituitary extract as a spawning- inducing agent. H. Rosenthal and S. Sang (Eds.), Research on Aquaculture, Eur. Maricult. Soc. Spec. Publ. No.8, Bredene, Belgium, 151-167.

Zohar, Y. 1986. Gonadotropin releasing hormone in spawning induction in teleosts: basic and applied considerations. Y. Zohar and H. Breton (Eds.), Reproduction in Fish, Basic and Applied Aspects in Endocrinology and Genetics, INRA, Paris, France, 47-61.

Zonneveld, N. 1984. The spawning season and the relation between temperature and stripping time of grass carp (Ctenopharyngodon idella Val.) in Egypt. Bamidgeh, 36: 21-27.