Immunomodulatory potential of a marine sponge Spongosorites halichondrioides (Dendy, 1905)

Marine sponges are animals belonging to the Phylum Porifera. They are well known for its medicinal value. However, to prove its efficiency for the clinical utilization, more experimental data will be beneficial. The present study involved the investigation of immunomodulatory activities of methanolic extract of marine sponge Spongosorites halichondriodes from western coast of India. The extract was studied for acute toxicity (OECD-425 guideline), Haemagglutinating antibody (HA) titre, delayed-type hypersensitivity (DTH) response and cyclophosphamide-induced myelosuppression for their immunomodulatory potential. The evaluation of immunomodulatory potential by oral administration of methanolic extract of marine sponge (200 mg/kg) evoked a significant decrease in total WBC count as compared to control, in antibody titre values, and also in delayed type hypersensitivity reaction induced by sheep red blood cells. Also it prevented myelosuppression in cyclophosphamide drug treated rats. The results obtained in the present study indicated that extract of marine sponge Spongosorites halichondrioides possesses immunosuppressant activity and can be studied further for isolation of the compounds which can be used for organ rejection purpose in future.

Anahtar Kelimeler:

siklofosfamit, sterol, hemaglutinasyon

PDF

___

Bao B, Sun Q, Yao X, Hong J, Lee CO, Sim CJ, Im KS, Jung JH. 2005 Cytotoxic bisindole alkaloids from a marine sponge Spongosorites sp. Journal of Natural Products, 68, 711-715. doi: 10.1021/np049577a

Bao B, Sun Q, Yao X, Hong J, Lee O, Cho HY, Im KS, Jung JH. 2007 Bisindole alkaloids of the topsentin and hamacanthin classes from a marine sponge Spongosorites sp. Journal of Natural Products 70, 2-8. doi: 10.1021/np060206z

Bao B, Zhang P, Lee Y, Hong J, Lee O, Jung JH. 2007 Monoindole Alkaloids from a Marine Sponge Spongosorites sp. Marine Drugs, 5, 31- 39. doi: 10.3390/md502031

Costantino V., Fattorusso E., Mangoni A. Di Rosa, M. and Ianaro, A. 1997 Glycolipids from Sponges. VI. Plakoside A and B, Two Unique Prenylated Glycosphingolipids with Immunosuppressive Activity from the Marine Sponge Plakortis simplex. J. Am. Chem. Soc. 119, 12465–12470. doi: 10.1021/ja9727225

Costantino V., Fattorusso E., Mangoni A. 2004. Glycolipids with Immunomodulating Activity from Marine Sponges, Taylor and Francis, New York: 555- 575,

Fusetani N., Sata N. and Matsunaga S. 1993 Isolation and Structure Elucidation of Erylusamine B, a New Class of Marine Natural Products, Which Blocked an IL-6 Receptor, from the Marine Sponge Erylus placenta Thiele. Tetrahedron Lett. 34 (25), 4067–4070. doi: 10.1016/S0040-4039(00)60617-2

Joseph B, Sujatha S. 2011 Pharmacologically Important Natural products from Marine Sponges. Journal of Natural Products, 4, 5-12.

Joshi C. S, Priya E. S, Venkatraman S. 2007 Acute and subacute toxicity studies on the polyherbal Antidiabetic Formulation Diakyur in Experimental Animal Models. Journal of Herbal Science, 53(2), 245-249. doi: 10.1248/jhs.53.245

OECD 2000 Guidance Document on Acute Oral Toxicity. Environmental Health and Safety Monograph Series on Testing and Assessment No.24.

OECD 2000 Guidance Document on the Recognition, Assessment and Use of Clinical Signs as Humane Endpoints for Experimental Animals Used in Safety Evaluation. Environmental Health and Safety Monograph Series on Testing and Assesment No 19.

Puri, A., Saxena, R.P., Saxena, K.C. 1993 Immunostimulant agents from Andrographis peniculata. Journal of Natural Product 56, 995–999. doi: 10.1021/np50097a002

Sata N., Asai N., Matsunaga, S. and Fuesetani N. 1994 Erylusamines, IL-6 Receptor Antagonists, from the Marine Sponge Erylus placenta. Tetrahedron 50 (4), 1105–1110. doi: 10.1016/S0040-4020(01)80821-8

Shivaprasad, H.N., Kharya, M.D., Rana, A.C., Mohan, S. 2006 Preliminary immunomodulatory activities of aqueous extract of Terminalia chebula. Pharmaceutical Biology 44, 32–34. doi: 10.1080/13880200500530542

Simpi C., Biradar P., Kalyane. N 2010 Immunomodulatory activities of ethyl acetate extracts of two marine sponges Gelliodes fibrosa and Tedania anhelans and brown algae Sargassum ilicifolium with reference to phagocytosis; Research Journal of Pharmaceutical, Biological and Chemical Sciences 1(2), 302-307.10)

Sipkema D, Franssen Maurice C.R, Osinga R, Tramper J. 2005 Marine Sponges as Pharmacy. Marine Biotechnology, 7, 142–162. doi: 10.1007/s10126-004-0405-5

Ziauddin, M., Phansalkar, N., Patki, P., Diwanay, S., Patwardhan, B. 1996 Studies on the immunomodulatory effects of Ashwagandha. Journal of Ethnopharmacology, 50, 69–76. doi: 10.1016/0378-8741(95)01318-0