GONCA ALAK, MUHAMMED ATAMANALP, AHMET TOPAL, HARUN ARSLAN, ERTAN ORUÇ, SERDAR ALTUN

Histopathological and biochemical effects of humic acid against cadmium toxicity in brown trout gills and muscles

Kadmiyum toksisitesine karşı humik asitin koruyucu etkisi biyokimyasal ve histopatolojik olarak kahverengi alabalık (Salmo trutta fario Linnaeus, 1792) solungaç ve kas dokularında araştırılmıştır. Balıklar kadmiyum ve/veya humik asite (2ppm Cd, 5 ppm humik asit) maruz bırakılmışlardır. Bu amaçla kahverengi alabalıkların kas ve solungaç dokularında glutatyon peroksidaz (GPX), süperoksit dismutaz (SOD), malondialdehit (MDA) düzeyleri incelenmiştir. Cd’a maruz kalan gruplarda GPX ve SOD enzim aktiviteleri kontrol grubuna kıyasla düşük değerler vermiştir (P0.05). Bununla beraber kadmiyum uygulanan gruplarda humik asit biyokimyasal zararı önlemede etkili olmamıştır. Cd kas ve solungaç dokularında histopatolojik olarak önemli artışlara neden olurken, Cd+HA’in birlikte uygulandığı grupta kas dokusu daha az etkilenmiştir. Bu sonuçlara göre, humik asit kas dokusunda histopatolojik olarak koruyucu etki gösterebilir

Humik asitin kahverengi alabalık solungaç ve kaslarında kadmiyum toksisitesine karşı histopatolojik ve biyokimyasal etkisi

It was biochemically and histopathologically investigated whether humic acid (HA) has protective effects on cadmium (Cd) toxicity on muscle and gills of brown trout (Salmo trutta fario Linnaeus, 1792). The brown trout were exposed to cadmium (2 ppm) and/or humic acid (5 ppm). For this purpose, levels of glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA) was investigated in muscle and gills tissues of brown trout. The activities of GPx and SOD in the tissues of fish exposed to Cd was significantly lower than the control groups (P<0.05). MDA levels did not increase in the groups exposed to cadmium (P>0.05). Hovewer, humic acid did not affect biochemical damage in cadmium group. Cd caused a significant increase in histopathological changes in muscle and gills tissues, but histopathological changes were lower in the muscle tissue of Cd+HA group. These results suggest that humic acid may counteract the cadmium toxicity in muscles tissue in histopathologic aspect.

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