Effect of different doses of mixed fertilizer onsome biogeochemical cycling bacterial population in carp culture pond

The effect of different doses of mixed fertilizer on the response of some biogeochemical cycling bacteria were examined using seven different doses (i.e, 105, 211, 422, 844, 1689, 3378 and 6757 g/tank/week) of mixed fertilizers with a fixed C : N : P ratio of 88.6 : 7.5 : 1. Advanced fry of Catla catla (1.2±0.04 g), Labeo bata (0.99±0.05 g), and Cyprinus carpio (1.3±0.06 g) were introduced at the rate of 16 fish/tank and reared for 120 days. Water samples were collected and monitored for bacterial population (HB, CDB, DNB and PSB), primary productivity, plankton, water quality parameters, and total phosphate in the sediment at ten days intervals. Number of bacteria (HB 15–270 x $10^3$ /ml, CDB 15–237 x $10^2$ /ml, DNB 5–221 x $10^2$ /ml and PSB 3–159 x $10^1$ /ml) increased directly with the increase in the fertilizer doses and showed maximum rate of bacterial growth at the fertilizer doses 48,000 kg/ha/yr. Three levels of responses to fertilizer doses - poor bacterial density and rate of fertilizer mineralization efficiency $(D_{12} to D_{48})$ , moderate bacterial density and highest rate of fertilizer mineralization efficiency $(>D_{48} to D_{192})$ and highest bacterial density but slow rate of fertilizer mineralization efficiency $(>D_{192} to D_{768})$ were observed. The fish growth tended to rise with the increase in fertilizer dose. Maximum fish growth was at D96 (96,000 kg/ha/year) and decline thereafter. Though the bacterial load increased with fertilizer doses, the primary productivity of phytoplankton and fish growth did not increased when fertilizer doses increased. Therefore, it can be concluded that fertilizer dose 48,000 kg/ha/yr is required to manipulate the optimum bacterial population and productivity of the aquaculture pond and application of excessive doses of fertilizer increases production cost and causes environmental pollution.

Anahtar Kelimeler:

çökelti, kültür balığı, su kalitesi, mikrobiyal flora, plankton, biyojeokimya, sazan balığı, büyüme oranı

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APHA (American Water Works Association and Water Pollution Control Federation). 1995. Standard Methods for the Examination of Water and Wastewater. 19th Edn. American Public Health Association, Washington, DC.

Austin, B. 1990. Methods in aquatic bacteriology. John Wiley and Sons, New York, 425 pp.

Bhakta, J.N. 2003. Fertilizer-microbial interactions in wastewater system: Inflence of fertilizer dose and stocking density of fish. PhD Thesis. University of Kalyani: Kalyani, India.

Boyd, C.E. 1995. Bottom Soils, Sediment, and Pond Aquaculture. Chapman and Hall, New York, 348 pp.

Das, S.K. and Jana, B.B. 1996. Pond fertilization through inorganic sources: An overview. Indian Journal of Fisheries, 43: 137-155.

Debeljak, L., Turk, M., Fasaic, K. and Popovic, J. 1990. Mineral fertilizers and fish production in carp ponds. In: R. Berka and V. Hilge (Eds.), Proceedings of the FAO-EIFAC Symposium on Production Enhancement in Still Water Pond Culture, Research Institute of Fish Culture and Hydrobiology, Vodnany, Czechoslovakia: 187-193.

Gaur, A.C., Dargan, K.S. and Neelakantan., K.S. 1995. Organic manure. Publication and Information Division, Indian Council of Agricultural Research, New Delhi, India, 159 pp.

Ghosh, M. and Chattopadhyay, N.R. 2005. Effects of carbon/nitrogen/phosphorus ratio on mineralizing bacterial population in aquaculture systems. Journal of Applied Aquaculture, 17(2): 85-98.

Goldman, J.C., Caron, D.A. and Dennett, M.R. 1987. Regulation of gross growth efficiency and ammonium regeneration in bacteria by substrate C : N ratio. Limnology and Oceanography, 32: 1239-1252.

Gomez, K.A. and Gomez, A.A. 1984. Statistical procedures for agricultural research. 2nd Edition, John Wiley and Sons, New York, 680 pp.

Jackson, M.L. 1967. Soil Chemical Analysis. Prentice Hall of India Pvt. Ltd., New Delhi, 498 pp.

Jana, B.B., Chakrabarti, P., Biswas, J.K. and Ganguly, S. 2001. Biogeochemical cycling bacteria as indices of pond fertilization: Importance of CNP ratios of input fertilizers. Journal of Applied Microbiology, 90: 733-740.

Jana, B.B. and De, U.K. 1990. Spatial and seasonal distribution of heterotrophic bacteria in pond water and sediments under different management practices. International Revue der Gesamten Hydrobiologie, 75: 639-648.

Jana, B.B. and De, U.K. 1993. Management induced variability of the bacterioplankton in fish farming ponds. Journal of Aquaculture in the Tropics, 8: 131-140.

Jana, B.B. and Patel, G.N. 1984. Spatial and seasonal variations of phosphate solubilizing bacteria in fish ponds of varying fish farming managements. Archives fiir Hydrobiologie, 10: 555-568.

Jana, B.B. and Patel, G.N. 1985. Distribution pattern denitrifying bacteria in fish ponds of differing farming managements. Archivesfiir Hydrobiologie, 103: 291-303.

Jana, B.B. and Patel, G.N. 1990. Pattern and magnitude of nitrate reduction in fish ponds differing in management practices. Aquacultura Hungarica (Szarvas), 6: 53-63.

Jana, B.B. and Roy, S.K. 1985a. Distribution patterns of protein mineralizing and ammonifying bacterial populations in fish-farming ponds under different management systems. Aquaculture, 44: 57-65.

Jana, B.B. and Roy, S.K. 1985b. Spatial and temporal changes of nitrifying bacterial population in fish ponds of different management practices. Journal of Applied Bacteriology, 59: 195-204.

Jana, B.B. and Roy, S.K. 1986. Seasonal and spatial distribution pattern of nitrogen fixing bacteria in fish ponds under different management systems. Hydrobiologia, 137: 45-54.

Jhingran, V. G. 1995. Fish and Fisheries of India. Hindustan Publishing Corporation New Delhi, India.

Milstein, A., Azim, M.E., Abdul, W.M. and Verdegem, M.C.J. 2003. The Effects of Periphyton, Fish and Fertilizer Dose on Biological Processes Affecting Water Quality in Earthen Fish Ponds. Environmental Biology of Fishes, 64 (3): 247-260.

Moav, R., Wohlfarth, G.W., Schroeder, G.L., Hulata, G. and Barash, H. 1977. Intensive polyculture of fish in freshwater ponds. Aquaculture, 10: 25-43.

Persson, T., Baath, E., Clarholm, M., Lundkvist, H., Soderstrom, B. and Sohlenius, B. 1980. Trophic structure, biomass dynamics and carbon metabolism of soil organisms in Scots pine forest. Ecological Bulletin, 32: 419-462.

Rodina, A.G. 1972. Methods in Aquatic Microbiology. In: R.R. Coiwell and M.S. Zambruski (Eds.), University Park Press, Baltimore, Butterworths, London: 461.

Schroeder, G.L. 1974. Use of fluid cowshed manure in fish ponds. Bamidgeh, 26(1): 84-96.

Tezuka, Y. 1990. Bacterial regeneration of ammonium and phosphate as affected by the carbon : nitrogen : phosphorus ratio of organic substrates. Microbial Ecology, 19: 227–238.

Vollenweider, R.A. 1974. A manual on methods for measuring primary production in aquatic environments. IBP. Blackwell Scientific Publications, Oxford, London, 225 pp.

Wolny, P. 1967. Fertilization of worm-water fish ponds in Europe. FAO Fish Report, 44: 64-81.