The effect of acid and alkaline pH shift processes on biochemical and physicochemical characteristics of protein isolates from bigeye snapper (Priacanthus tayenus) head by-product (HB) was investigated. Approximately 30% protein yield with the significant reduction of lipid and ash (P<0.05) was obtained from both processes. However, partial denaturation of resulting isolates was noticeable in both processes as shown by the decreases in reactive SH content and Ca2+-ATPase activity (P<0.05). Acid process recovered less total pigments with lowered heme iron content resulting in a greater whiteness of the isolate (P<0.05). The highest haem-globin destruction indicated by the disappearance of a soret peak of myoglobin spectra was noticeable in the acid process. The oxidation of residual lipids in both protein isolates measured by TBARS was markedly lowered than in HB (P<0.05) even though the PV was not significantly different among treatments (P>0.05). Both acid and alkaline methods effectively removed TVB-N and fishy odour and lowered the TCA-soluble peptides from HB (P<0.05). Therefore, the pH shift processing can be used as a powerful tool to recover proteins from HB. However, properties of the protein isolate were influenced by solubilisation method. Acid process gave a protein isolate with better overall quality compared to alkaline version.
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AOAC. 2000. Official Methods of Analysis, 16th edition, Association Washington, DC. Analysis Chemists,
Asgharzadeh, A., Shabanpour, B., Aubourg, S.P. and Hosseini, H. 2010. Chemical changes in silver carp (Hypophthalmichthys molitrix) minced muscle during frozen storage: Effect of a previous washing process. Grasas 10.3989/gya.087109 61: 95-101. doi:
Bates, L., Ames, J.M., Mac Dougall, D.B. and Taylor, P.C. 1998. Laboratory reaction cell to model Maillard reaction colour development in a starch-glucose- lysine system. Journal of Food Science, 63: 1991- 1996. doi: 10.1111/j.1365-2621.1998.tb15840.x
Bechtel, P.J., Sathivel, S. and Oliveira, A.C.M. 2006. Production and characterization of alkali extracted protein powders from pollock and salmon heads. Second Joint Trans-Atlantic Fisheries Technology Conference. 2006 Oct 29. Quebec City, Canada: Trans-Atlantic Fisheries Technology (AFTC), West European Fish Technologists Assn. (WEFTA).
Benjakul, S., Seymour, T.A., Morrissey, M.T. and An, H. 1997. Physicochemical changes in Pacific whiting muscle proteins during iced storage. Journal of Food Science, 2621.1997.tb15445.x doi: 10.1111/j.1365
Buege, J.A. and Aust, S.D. 1978. Microsomal lipid peroxidation methods. Methods in Enzymology, 52: 302-310. doi: 10.1016/S0076-6879(78)52032-6
Chaijan, M., Benjakul, S., Visessanguan, W. and Faustman, C. 2005. Changes of pigments and colour in sardine (Sardinella gibbosa) and mackerel (Rastrelliger kanagurta) muscle during iced storage. Food Chemistry, 10.1016/j.foodchem.2004.10.035 607-617. Doi:
Chaijan, M. and Undeland, I. 2015. Development of a new method for determination of total haem protein in fish muscle. Food Chemistry, 173: 1133-1141. doi: 10.1016/j.foodchem.2014.11.010
Chen, H.H., Chiu, E.M. and Huang, J.R. 1997. Colour and gel forming properties of horse mackerel (Trachurus japonucus) as related to washing conditions. Journal of Food Science, 62: 985-991. doi: 10.1111/j.1365- 2621.1997.tb15021.x
Chen, Y.C. and Jaczynski, J. 2007. Protein recovery from rainbow trout (Oncorhynchus mykiss) processing by- products via isoelectric solubilisation/precipitation and its gelation properties as affected by functional additives. Chemistry, 55: 9079-9088. doi: 10.1021/jf071992w
Cheng, J.-H. and Ockerman, H.W. 2004. Effect of ascorbic acid with tumbling on lipid oxidation of precooked roast beef. Journal of Muscle Foods, 15: 83-93. doi: 10.1111/j.1745-4573.2004.tb00713.x
Chomnawang, C. and Yongsawatdigul, J. 2013. Protein recovery of tilapia frame by-products by pH-shift method. Technology, 10.1080/10498850.2011.629077 Food Product 112-120. doi:
Ellman, G.L. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics, 82: 70-77. doi: -90090599861000310.1016 690090599861000310.1016 /10.1016 -000310.1016
Fiske, C.H. and Subbarow, Y. 1925. The colourimetric determination of phosphorus. Journal of Biological Chemistry, 66: 375-400.
Gehring, C.K., Gigliotti, J.C., Moritz, J.S., Tou, J.C. and Jaczynski, J. 2011. Functional and nutritional characteristics of proteins and lipids recovered by isoelectric processing of fish by-products and low- value fish - A review. Food Chemistry, 124: 422-431. doi: 10.1016j.foodchem.2010.06.078 /
Gildberg, A. 2004. Digestive enzyme activities in starved pre-slaughter farmed and wild-captured Atlantic cod (Gadus morhua). Aquaculture, 238: 343-353. doi: 10.1016/S0044-8486(04)00210-8
Hornsey, H.C. 1956. The colour of cooked cured pork. I. Estimation of nitric oxide-haem pigments. Journal of the Science of Food and Agriculture, 7: 534-540. doi: /10.1002jsfa.2740070804
Hultin, H.O. and Kelleher, S.D. 1999. Process for isolating a protein composition from a muscle source and protein composition. Advanced Protein Technologies Inc., assignee. US Pat. No. 6,005,073.
Hultin, H.O. and Kelleher, S.D. 2000. Surimi processing from dark muscle fish. In J.W. Park (Ed.), Surimi and Surimi Seafood, Marcel Dekker, New York: 59-77.
Kristinsson, H.G. 2002. Acid unfolding of flounder haemoglobin: Evidence for a molten globular state with enhanced pro-oxidative activity. Journal of Agricultural and Food Chemistry, 50: 7669-7676. doi: /10.1021jf0256788
Kristinsson, H.G. and Hultin, H.O. 2003. Changes in conformation and subunit assembly of cod myosin at low and high pH and after subsequent refolding. Journal of Agricultural and Food Chemistry, 51: 7187-7196. doi: 10.1021jf026193m /
Kristinsson, H.G. and Liang, T. 2006. Effect of pH-shift processing and surimi processing on Atlantic croaker (Micropogonias undulates) muscle proteins. Journal of Food Science, 7: 298-306. doi: 10.1111/j.1750- 3841.2006.00046.x
Liu, G., Xiong, Y.L. and Butterfield, D.A. 2000. Chemical, physical, and gel-forming properties of oxidised myofibrils and whey- and soy protein isolates. Journal of Food Science, 65: 811-818. doi: 10.1111/j.1365- 2621.2000.tb13592.x
Low, L.K. and Ng. C.S. 1978. Determination of peroxide value. In H. Hasegawa (Ed.), Laboratory Manual on Analytical Methods and Procedures for Fish and Fish Products, Marine Fisheries Research Department, Southeast Asian Fisheries Development Centre, Singapore: C7.1-C7.3.
Lowry, O.H., Rosebrough, N.J., Farr, L.A. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193: 265- 275. doi: 10.1021jf021099r /
Maqsood, S. and Benjakul, S. 2011. Effect of bleeding on lipid oxidation and quality changes of Asian seabass (Lates calcarifer) muscle during iced storage. Food Chemistry, 10.1016/j.foodchem.2010.06.055 459-467. doi:
Marmon, S.K. and Undeland, I. 2010. Protein isolation from gutted herring (Clupea harengus) using pH-shift processes. Journal of Agricultural and Food Chemistry, 58: 10480-10486. doi: 10.1021jf101057q /
Matak, K.E., Tahergorabi, R. and Jaczynsk, J. 2015. A review: Protein isolates recovered by isoelectric solubilisation/precipitation processing from muscle food by-products as a component of nutraceutical foods. Food Research International. In press. doi: 10. 1016/ j.foodres.2015.05.048
Ng, C.S. 1987. Determination of trimethylamine oxide (TMAO-N), trimethylamine (TMA-N), total volatile basic nitrogen (TVB-N) by Conway's method. In H. Hasegawa (Ed.), Laboratory Manual on Analytical Methods and Procedures for Fish and Fish Products, Marine Fisheries Research Department, Southeast Asian Fisheries Development Centre, Singapore: 1-8.
Nunes, M.L., Batista, I. and De Campos, R.M. 1992. Physical, chemical and sensory analysis of sardine (Sardina pilchardus) stored in ice. Journal of the Science of Food and Agriculture, 59: 37-43. doi: 10.1002/jsfa.2740590106
Pires, C., Batista, I., Fradinho, P. and Costa, S. 2009. Utilization of alkaline-recovered proteins from Cape hake by-products in the preparation of Frankfurter- type fish sausages. Journal of Aquatic Food Product Technology, 10.1080/10498850802629135 170-190. doi:
Rawdkuen, S., Sai-Ut, S., Khamsorn, S., Chaijan, M. and Benjakul, S. 2009. Biochemical and gelling properties of tilapia surimi and protein recovered using an acid- alkaline process. Food Chemistry, 112: 112-119. doi: 10.1016/j.foodchem.2008.05.047
Richards, M.P., Kelleher, S.D. and Hultin, H.O. 1998. Effect of washing with or without antioxidants on quality refrigerated Agricultural and Food Chemistry, 46: 4363-4371. doi: /10.1021jf980311h fillets during and storage. Journal of
Riebroy, S., Benjakul, S., Visessanguan, W. and Tanaka, M. 2007. Effect of iced storage of bigeye snapper (Priacanthus tayenus) on the chemical composition, properties and acceptability of Som-fug, a fermented Thai fish mince. Food Chemistry, 102: 270-280. doi: 10.1016/j.foodchem.2006.05.017
Sae-leaw, T., Benjakul, S., Gokoglu, N. and Nalinanon, S. 2013. Changes in lipids and fishy odour development in skin from Nile tilapia (Oreochromis niloticus) stored in ice. Food Chemistry, 141: 2466-2472. doi: /10.1016j.foodchem.2013.05.049
Schaich, K. and Karel, M. 1975. Free radical reactions of peroxidising lipid with amino acids and proteins: and ESR 10.1007/BF02532846 11: 392-400. doi:
Shaviklo, G.R., Arason, S., Thorkelsson, G., Sveinsdottir, K. and Martinsdottir, E. 2010. Sensory attributes of haddock balls affected by added fish protein isolate and frozen storage. Journal of Sensory Studies, 25: 316-331. doi: 10.1111/j.1745-459X.2009.00260.x
Shaviklo, A.R, Moradinezhad, N., Abolghasemi, S.J. and Rafipour, F. 2015. Quality of silver carp burger incorporated with protein isolated from tuna by product. Oral presentation. The First Middle East and Central Asia Aquaculture Conference, December 14- 16, Tehran, Iran, Proceedings and Book of Abstracts, pp.413
Stadtman, E.R. and Oliver, C.N. 1991. Metal-catalysed oxidation Chemistry, 266: 2005-2008. Journal of Biological
Steel, R.G.D. and Torrie, J.H. 1980. Principle and Procedure of Statistics. McGraw-Hill, New York, 633 pp.
Tahergorabi, R., Beamer, S.K., Matak, K.E. and Jaczynski, J. 2012. Functional food products made from fish protein solubilisation/precipitation. LWT-Food Science and Technology, 10.1016/j.lwt.2012.02.018 with isoelectric 48: 89-95. doi:
Taskaya, L., Chen, Y.I.C., Beamer, S., Tou, J.C. and Jaczynski, J. 2009. Compositional characteristics of materials recovered from whole gutted silver carp (Hypophthalmichthys solubilisation/precipitation. Journal of Agricultural and 10.1021/jf803974q using isoelectric Chemistry, 57: 4259-4266. doi:
Undeland, I.A., Hall, G., Wendin, K., Gangby, I. and Rutgersson, A. 2005. Preventing lipid oxidation during recovery of functional proteins from herring (Clupea harengus) fillets by an acid solubilisation process. Journal of Agricultural and Food Chemistry, 53: 5625-5634. doi: 10.1021/jf0404445
Undeland, I., Hultin, H.O. and Richards, M.P. 2003. Aqueous extracts from some muscles inhibit haemoglobin-mediated oxidation of cod muscle membrane lipids. Journal of Agricultural and Food Chemistry, 51: 3111-3119. doi: 10.1021jf020770p
Valdimarson, G. and James, D. 2001. World fisheries- / utilisation of catches. Ocean & Coastal Management, 44: 619-633. doi: 10.1016S00072 / -0964 )56910964 (0156910964 -000720156910964 2000720156910964
Zayas, J. F. 1997. Functionality of Proteins in Food. Spring- Verlag, Berlin, 373 pp.