A study on the milt quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843) in Atatürk Dam Lake, Southeastern Turkey

he aim of this study was to determine the milt quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843). Milt was collected twice by abdominal massage during the spawning seasons of the species. Volume, forward motility, duration of motility, milt concentration and pH were determined in milt collected. Body weights and lenghts of brood fish were also measured. Milt volume (ml), forward motility (%), the duration of motility (sec), concentration (x109cell/ml) and pH values were 1.22±0.22 and 0.80±0.06, 73.25±5.15 and 55.50±4.59, 90.80±10.40 and 175.80±17.00, 6.06±0.90 and 11.29±1.46, 7.99±0.03 and 8.07±0.12 in O. mykiss and C. luteus, respectively. The spermatozoa concentration of O. mykiss, and duration of motility, spermatozoa concentration and sperm pH of C. luteus were affected significantly by milt stripping dates (P<0.05, P<0.01). The results suggested that the spermatozoa concentration of O. mykiss, and duration of motility, spermatozoa concentration and sperm pH of C. luteus were affected by stripping dates.

Anahtar Kelimeler:

Türkiye, döllenme, gökkuşağı alabalığı, spermatozoa, motilite

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1. Aas, G.H., Refstie, T. and Gjerde, B. 1991. Evaluation of milt quality of Atlantic Salmon. Aquaculture, 95: 125-132.

2. Aral, F., Doğu, Z., Selçuk, B., Taş, M. and Kılıç, S.Ö. 2004. Determination of the spermatological properties at first spawning season of the young male rainbow trouts (Oncorhynchus mykiss W., 1792) reared in floating cages in Atatürk Dam Lake. J. Anim. Vet. Adv., 3: 542-546.

3. Babiak, I., Glogowski, J., Brzuska, E., Szumiec, J. and Adamek, J. 1997. Cryopreservation of sperm of common carp, Cyprinus carpio L. Aquacult. Res., 28: 567-571.

4. Babiak, I., Fraser, L., Dobosz, S., Goryczko, K., Kuzminski H. and Strzezek, J. 1999. Computer-controlled freezing of rainbow trout Onchorhynchus mykiss (Walbaum) spermatozoa for routine programmes. Aquacult. Res., 30: 707-710.

5. Babiak, I., Glogowski, J., Dobosz, S., Kuzminski, H. and Goryczko, K. 2002. Semen from rainbow trout produced using cryopreserved spermatozoa is more suitable for cryopreservation. J. Fish. Biol., 60: 561— 570.

6. Basavaraja, N. and Hegde, N.S. 2004. Cryopreservation of the endangered mahseer {Tor khudree) spermatozoa: I. effect of extender composition, cryoprotectants, dilution ratio, and storage period on post-thaw viability. Cryobiology, 49: 149-156.

7. Billard, R. 1983. Effects of ceolomic and seminal fluids and various saline diluents on the fertilizing ability of spermatozoa in the rainbow trout, Salmo gairdneri. J. Reprod. Fert., 68: 77-84.

8. Billard, R., Cosson, J. and Crim, L.W. 1993. Motility of fresh and aged halibut sperm. Aquat. Living Resou., 6: 67-75.

9. Billard, R., Cosson, J., Perchec, G, and Linhart, O. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture, 124: 95-112.

10.Bloom, H.J. and Ottobre, S.J. 2001. Gossypol isomers bind specifically to blood plasma protein and spermatozoa of rainbow trout fed diets containing cottonseed meal. Biochim. Biophys. Acta., 15: 37-42.

11.Büyükhatipoğlu, Ş. and Holtz, W. 1984. Sperm output in trout: effect of age, timing and frequency of stripping and presence of females. Aquaculture, 37: 63-71.

12.Cabrita, E., Anel, L. and Herraez, P.M. 2001. Effect of external cryoprotectans as membrane stabilizers on cryopreserved trout sperm. Theriogenology, 56: 623-635.

13.Ciereszko, A. and Dabrowski, K. 1993. Estimation of sperm concentration of rainbow-trout, whitefish and yellow perch using a spectrophotometric technique. Aquaculture, 109: 367-373.

14.Geldiay,'R. and Balık, S. 1999. Türkiye Tatlısu Balıkları. E.Ü. Su Ürünleri Fakültesi Yayınları No: 46, İzmir, '532 pp. .

15.Horvâth, A., Miskolczi, E. and Urbânyi, B. 2003. Cryopreservation of common carp sperm. Aquat. Living Resour., 16: 457-460.

16.Krasznai, Z., Marian, T., Balkay, L., Gasper R. Jr. and Tron, L. 1995. Potassium channels requlate hypo-osmotic shock-induced motility of common carp, Cyprinus carpio, sperm. Aquaculture, 129: 123-128.

17.Lahnsteiner, F., Berger, B., Weismann, T. and Patzner, R.A. 1998. Determination of semen quality of the rainbow trout by sperm motility, seminal plasma parameters and spennatozoal metabolism. Aquaculture, 163: 163-181.

18.Liley, R.N., Tamkee, P., Tsai, R. and Hoysak, J.D. 2002. Fertilization dynamics in rainbow trout {Oncorhynchus mykiss): Effect of male age, social experience, and sperm concentration and motility on in vitro fertilization. Can. J. Fish. Aquat. Sci., 59: 144-152.

19.Linhart, O., Rodina, M. and Jacky Cosson, J. 2000. Cryopreservation of sperm in common carp Cyprinus carpio: Sperm motility and hatching success of embryos. Cryobiology, 41: 241-250.

20.Linhart, O., Mims, S.D. and Shelton, W.L. 1995. Motility of spermatozoa from Shovelnose Sturgeon, Scaphirhynchus platorynchus, and Paddlefish, Polyodon spathula. J. Fish Biol., 47: 902-909.

21.Moon, S.H., Kwon, Y.J., Lee, KJ. and Chang, J.Y. 2003. Increased plasma 17-hydroxyprogesterone and milt production in response to gonadotropin-releasing hormone agonist in captive male starry flounder, Platichthys stellatus. Aquaculture, 218: 703-716,

22.Morisawa, M. and Suzuki, K. 1980. Osmolality and potassium ions: Their roles in initiation of sperm motility in teleosts. Science, 210: 1145-1147.

23.Morisawa, M., Suzuki, K. and Morisawa, S. 1988. Effects of potassium and osmolality on spennatozoan motility of salmonid fishes. J. Exp. Biol., 107: 105-113.

24.Munkittrick, R.K. and Moccia, D.R. 1987. Seasonal changes in the quality of rainbow trout {Salmo gairdneri) Semen: Effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma constituents. Aquaculture, 64: 147-156.

25.Percheca, G., Cossonb, J., Billard, F. and Billard, R. 1995. Degradation of the quality of carp sperm by urine contamination during stripping. Aquaculture, 129: , 133-137.

26.Pool, R.W. and Dillane, G.M. 1998. Estimation of sperm concentration of wild and reconditioned brown trout, Salmo trutta L. Aquaculture Res., 29(6): 439-445.

27.Ricker, W.E. 1975. Computation and Interpretation of Biological Statistics of Fish Population. Bull. Fish. Board Can., 191 pp.

28.Şevik, R. and Yüksel, M. 1997. Atarük Baraj Gölü'nde yaşayan Carasobarbus luteus (Heckel, 1843) üzerine araştırmalar II. IX. Ulusal Su Ürünleri Sempozyumu, (17-19 Eylül), İsparta: 216-221.

29.Suzuki, R. 1995. Sperm activation and aggregation during fertilization in some fishes: III non species specificity ' of stimulating factor. Anot. Zool. Jap., 32: 105-111.

30.Tekin, N,, Seçer, S., Akçay, E., Bozkurt, Y. and Kayam, S. 2003. Gökkuşağı alabalıklarında {Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Türk J. Vet. Anim. Sci., 27: 37-44.

31.Yuchi, S.W. and Chang, F.C. 1997. 17a, 2013, 21-trihydroxy-4-pregnen-3-one and 17a, 20B-dihydroxy-4-pregnen-3-one stimulated spermiation in protandrous black porgy, Âcanthopagrus schlegeli. FishPhysiol. Biochem., 17: 187-193.