Agrobacterıum- mediated transformatıon in linseed (Linum usitatissimum L.)

Agrobacterıum- mediated transformatıon in linseed (Linum usitatissimum L.)

An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. TTiis research summarizes a simple culture method to obtain rooted plants from stem pieces of linseed cultivar Antares within 3-4 months. Here, we also present our results on transformation experiments carried out with 7-day-old stem explants and Agrobacterium tumefaciens EHA105 strain. According to molecular examination 14 transgenic plants of 276 (5.1%) examined plants were obtained.

___

  • Battraw MJ, Hall TC, 1990. Histochemical analysis of CaMV 35S. Plant Mol Biol 15:527-538.
  • Bretagne, B., Chupeau, MC, Chupeau, Y. and G. Fouilloux., 1994. Improved flax regeneration from hypocotyls using thidiazuron as a cytokine source. Plant Cell Rep. 14:120-124.
  • Dong, J. Z. and A. McHughen., 1993. An improved procedure for production of transgenic flax plants using Agrobacterium tumefaciens. Plant Sci. 88: 61-71.
  • Janssen B, Gardner RC, (1989) Localized transient expression of GUS in leaf disks following co-cultivation with Agrobacterium. Plant Mol Biol 14:61-72.
  • Jefferson, R.A., 1987. Assaying chimeric genes in plants: the GUS gene fusion system.Plant Molecular Biology Reporter 5:3 87-405.
  • Jefferson, R.A., Kavanagh, T. A. and Bevan, M. W., 1987. GUS fusions: (3- glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO Journal 6:3901-3907.
  • Kosugi, S., Ohashi, Y, Nakajima, K. and Y Arai., 1990. An improved assay for Pglucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous P-glucuronidase activity. Plant Science 70: 133-140.
  • MIynarova, L., Bauer, M., Nap, J. P. and A. Pretova., 1994. High efficiency Agrobacterium mediated gene transfer to flax. Plant Cell rep. 13: 282-285.
  • Potrykus I. and G. Spangenberg., 1995. Gene transfer to plants, Springer Publications,The Netherlands.