Adventitious shoot regeneration from petioles with leaflets in potato (Solanum tunerosum l.)

Adventitious shoot regeneration from petioles with leaflets in potato (Solanum tunerosum l.)

Adventitious shoot regeneration of 4 potato genotypes in 4 different regeneration media (RM) was studied. Petioles with leaflets obtained from node culture were used as explants and adventitious shoot formation and rooting of shoots were observed. Results showed significant genotype differences but no difference among RM for shoot production was found. Rooting of adventitious shoots was highly affected RM. Genotype Nif gave the highest means for number of shoots (4.6), number of roots (4.7) and total number of shoots (13.9).

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  • FAOSTAT. 2005. PRODSTAT module. http://faostat.fao.org/site/526/default.aspx
  • Hoekema, A., MJ. Huisman, L Molendijik, P.J.M. van der Elzan and BJ.C. Cornelissen. 1989. The genetic engineering of two commercial potato cultivars to potato virus X. Biotechnology, 7: 273-278.
  • Hulme, J.S., S. Elaine, S. Higgins and R. Shields. 1992. An efficient genotypeindependent method for regeneration of potato plants from leaf tissue. Plant Cell Tiss Org Cult 31: 161-167.
  • Ishida, B.K. ,G.W. Snyder and W.R.Belknap.l989.The use of in vitro grown microtuber discs in Agrobacterium mediated transformation of Russet Burbank and Lemhi Russet potatoes. Plant Cell Rep 8: 325-328.
  • Jacobsen, E. 1977. Doubling dihaploid potato clones via leaf tissue culture. Z. für Pflanzenzüchtung, 80: 80-82.
  • Murashige, T. and F. Skoog.1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473-497. Newell,C.A.,R.Rozman,M.A.Hincae,E.C.Lawson,L.Haley,P.Sanders, W.Kaniewski,N.E.Turner,R.B. Horsh and R.T.Fraley. 1991. Agrobacterium-mediated transformation of Solarium tuberosum cv. Russet Burbank. Plant Cell Rep 10: 30-34.
  • Park, Y.D., D.H. Ronis, A.A. Boe, and Z.M. Cheng. 1995. Plant regeneration from leaf tissue of four North Dakota genotypes of potato {Solarium tuberosum L.) Am Potato Res 72: 329-338.
  • Roest, S. and G.S. Bokelman. 1976. Vegetative propagation of Solarium tuberosum L. in vitro. Potato Res. 19:173-178.
  • Sherman, S. and M.W.Bewan.l988.A rapid transformation method for Solanum tuberosum using binary Agrobacterium tumafaciens vectors. Plant Cell Rep., 7:13-16
  • Snyder, G.W. and Belknap.l993.A modified method for routine Agrobacteriummediated transformation in vitro grown potato microtubers. Plant Cell Rep 12: 324-327.
  • Steel, R.G.D. and C.H.Torrie.1980. Principles and Procedures of Statistics. McGraw-Hill Book Company Inc. NY
  • Wheeler, V. A., N. E. Evans ,D. Fougler, K. J. Webb , A. Karp ,J. Franklin ,and S.W.J. Bright. 1985.Shoot formation from explant cultures of fourteen potato cultivars and studies of cytology and morphology of regenerated plants. Annals of Botany, 55 : 321-329.
  • Yee, S., B. Stevens, S. Coleman, J.E.A. Seabrook and X.Q. Li. 2001. In vitro regeneration of leaflets and petioles in potato. Am J Potato Res. 78:151-157.