An accurate, simple, reproducible and sensitive method for the determination of paracetamol, caffeine and dipyrone was developed and validated. Paracetamol, caffeine and dipyrone were separated using a m -Bondapack C 8 column by isocratic elution with a flow rate of 1.0 ml/min. The mobile phase composition was 0.01 M KH2PO4- -methanol-acetonitrile-isopropyl alcohol (420: 20: 30: 30) (v/v/v/v) and spectrophotometric detection was carried out at 215 nm. The linear range of determination for paracetamol, caffeine and dipyrone were 0.409-400 m g/ml, 0.151-200 m g/ml and 0.233-600 m g/ml, respectively. The method was shown to be linear, reproducible, specific, sensitive and rugged.

An accurate, simple, reproducible and sensitive method for the determination of paracetamol, caffeine and dipyrone was developed and validated. Paracetamol, caffeine and dipyrone were separated using a m -Bondapack C 8 column by isocratic elution with a flow rate of 1.0 ml/min. The mobile phase composition was 0.01 M KH2PO4- -methanol-acetonitrile-isopropyl alcohol (420: 20: 30: 30) (v/v/v/v) and spectrophotometric detection was carried out at 215 nm. The linear range of determination for paracetamol, caffeine and dipyrone were 0.409-400 m g/ml, 0.151-200 m g/ml and 0.233-600 m g/ml, respectively. The method was shown to be linear, reproducible, specific, sensitive and rugged.