Idress F. AL-MOMANI

7474

Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC

A high-performance liquid chromatographic procedure is presented for the determination of hydrochlorothiazide (HCT) and enalapril maleate (EM) in pharmaceutical tablets. An aliquot of the sample is dissolved in 15% acetonitrile (ACN) containing thiophylline as an internal standard and chromatographed on a Supelcosil LC-8 (5 m m), (150 mm x 4.6 mm i.d.) column. The mobile phase was 3.0 mM tetrabutylammoniumhydrogen sulfate (TBAHS) in ACN/water/triethylamine (TEA), (14, 85.6, 6.4 V/V) adjusted to pH 4.1 by glacial acetic acid. The detection was at 220 nm. The method was tested for linearity, accuracy, recovery, and specificity.
Anahtar Kelimeler:

HPLC, reverse phase, hydrochlorothiazide, enalapril maleate, stability, analysis

Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC

A high-performance liquid chromatographic procedure is presented for the determination of hydrochlorothiazide (HCT) and enalapril maleate (EM) in pharmaceutical tablets. An aliquot of the sample is dissolved in 15% acetonitrile (ACN) containing thiophylline as an internal standard and chromatographed on a Supelcosil LC-8 (5 m m), (150 mm x 4.6 mm i.d.) column. The mobile phase was 3.0 mM tetrabutylammoniumhydrogen sulfate (TBAHS) in ACN/water/triethylamine (TEA), (14, 85.6, 6.4 V/V) adjusted to pH 4.1 by glacial acetic acid. The detection was at 220 nm. The method was tested for linearity, accuracy, recovery, and specificity.
Keywords:

HPLC, reverse phase, hydrochlorothiazide, enalapril maleate, stability, analysis,

PDF
Turkish Journal of Chemistry-Cover
  • ISSN: 1300-0527
  • Yayın Aralığı: 6
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Evidence for a Hybrid Ping Pong-Semirandom Mechanism for Human Jejunal Glutathione Disulfide Reductase

Nazmi ÖZER, İzzet Hamdi ÖĞÜŞ

Voltammetric and Polarographic Studies of Eriochrome Black T - Nickel(II) Complex

Osman ÇAKIR, Emine ÇOŞKUN, Ender Biçer & Semiha ÇAKIR

Photocuring of Acrylate Oligomers in the Presence of Vinyl Phosphonic Acids as a Flame Retarding Monomer and the Properties of the Cured Films

Tülin İMAMOĞLU, Yusuf YAĞCI

Spectrophotometric Determination of Cobalt, Nickel Palladium, Copper, Ruthenium and Molybdenum Using Sodium Isoamylxanthate in Presence of Surfactants

A. K. MALIK, K. N. KAUL, B. S. LARK, W. FAUBEL, A. L. J. RAO

Determinetion of the thermodynamic properties of poly [ 2-(3-phenyl-3-methylcyclobutyl)-2-hydroxyethyl methacrylate-co-methacrylic acid ] at infinite dilution by inverse gas chromatography

Kadir DEMİRELLİ, İsmet KAYA

The Sorption of Metals on the Polysulfone Cation Exchange Membranes

M. ERSÖZ, A. ÇİMEN, I. H. GÜĞÜL, B. LEYLEK, S. YILDIZ

Fluorimetric detection of insulin in the presence of Eu (III) - { Pyridine-2,6-Dicarboxylate } tris complex

Serdar ATEŞ, Elmas GÖK

Synthesis and Structure of Systems Containing Pyramidalized and Strained Double Bond: An Investigation on the Cycloaddition Reactions of cis- and trans-3,8-dicarbomethoxy-3,8-dihydroheptalene

Synthesis and Structure of Systems Conta And

Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC

Idress F. AL-MOMANI

Electrorheological properties of suspensions prepared from polystyrene-block-polyisoprene copolymer

Mustafa YAVUZ, Yılmaz YILDIRIR, Halil İbrahim ÜNAL