Adsorption of cellulase on poly(2-hydroxyethyl methacrylate) cryogels containingphenylalanine

The aim of this study was to prepare a supermacroporous cryogel that can be used for the adsorption ofcellulase. The macroporous cryogel of poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-phenylalanine) [p(HEMA-MAPA)] was prepared by copolymerization of 2-hydroxyethyl methacrylate (HEMA) with a functional monomer of N-methacryloyl-l-phenylalanine (MAPA). The cryogel was characterized by scanning electron microscopy, Fourier transforminfrared spectroscopy, and swelling tests. The effects of several parameters such as medium pH, temperature, ionicstrength, and ow rate on cellulase adsorption were also investigated. Maximum cellulase adsorption was observedat 25◦C and pH 4.0. Furthermore, adsorbed cellulase was desorbed from the cryogel by using 1.0 M NaCl. Thep(HEMA-MAPA) cryogel could be used many times without the cellulase adsorption capacity decreasing significantly.

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