Üç kavak türünün çeliklerinde köklenme sırasındaki peroksidaz ve IAA-oksidaz aktiviteleri

Peroxidase (PO: E. C 1.11.1.7) and IAA. Oxidase (IAA-O) enzyme activities were investigated during rooting in cuttings of Populus nigra L., Populus alba L. and Populus tremula L. (Salicaceae), and the relations between enzyme activities and rooting ability were examined comparatively. PO activity started to increase the early stages of primordium formation and reached the highest level before root emergence. However, no apparent correlation was found between the activity of PO and the ability of the cuttings to form roots. IAA-O activity was observed only in root cuttings of P. alba and P. nigra. Activity increased after day 6 and reached the highest level at the stages of root emergence. A positive correlation between rooting and IAA-O activity was observed.

Peroxidase and IAA- oxidase activities during rooting in cuttings of three poplar species

Peroxidase (PO: E. C 1.11.1.7) and IAA. Oxidase (IAA-O) enzyme activities were investigated during rooting in cuttings of Populus nigra L., Populus alba L. and Populus tremula L. (Salicaceae), and the relations between enzyme activities and rooting ability were examined comparatively. PO activity started to increase the early stages of primordium formation and reached the highest level before root emergence. However, no apparent correlation was found between the activity of PO and the ability of the cuttings to form roots. IAA-O activity was observed only in root cuttings of P. alba and P. nigra. Activity increased after day 6 and reached the highest level at the stages of root emergence. A positive correlation between rooting and IAA-O activity was observed.

___

  • 1.Galston, A. W., J. Bonner, and R.S. Baker . Flavoprotein andPeroxidase as components of the Indoleacetic acid peroxidasesystem of peas. Arch. Biophys. 42:456-470 (1953).
  • 2.Dalet F, and Cornu D. Lignification level and Peroxidase activityduring in vitro rooting of Prunus avium. Can. J. Box. 67: 2182-2186 (1988).
  • 3.Ingemarsson B. S. M. Ethylene effects on Peroxidases and cellgrowth patterns in Picea abies hypocotyl cutting. Physio. Plant.94: 211-218 (1995).
  • 4.Nkang A., Effect of cyanide Pretreatment on Peroxidase Activity inGerminating Seeds of Guilfoylia monostylis. J. Plant Physiol. vol.149 pp.3-8 (1996)
  • 5.Pythoud F. and Buchala A. J., Peroxidase activity and adventitiousrooting in cuttings of Populus tremula. Plant Physiol. Biochem.27(4) 503-510 (1989)
  • 6.Al Barazi, Z., and Schwabe W.W. The possible involvement ofployphenol-oxidase and the auxin-oxidase system in rootformation and development in cuttings of Pistacia vera. J. of Hort.Scie. 59, 453-461 (1984).
  • 7.Battaccharya N. C., Kaur N. P. and Nanda K. K. Transient inisoperoxidases during rooting of etiolated stem segments ofPopulus nigra. Bichem. Physiol. Pflanzen 167, 159-164 (1975)
  • 8.Gorter C. T. Auxin synergists in the rooting of cuttings. Physiol.plant., 22, 497-502 (1969).
  • 9.Pilet, P. E. Auxin and process of aging in root cells in: Fourthinternational conference of plant growth regulation. Iowa StateUniv. Press. Amer. pp. 167-179 (1961).
  • 10.Bolduc, R. J; Cherry, J. H; Blair, B. O. Increase in indoleaceticAcid-oxidase Activity of winter wheat by cold Treatment andGibberellic Acid. Plant. Physiol. 45, 461-464 (1970).
  • 11.Mancousin, C:, Favre, J. M. and Thomas J. Early Changes in auxinand Ethylene Production in vine cutting before adventitiousrooting. Plant Cell, Tissue and Organ Culture,19: 235-242 (1989)
  • 12.Srivastava,O. P., and Haystee R. B. An interrelationship amongperoxidase, IAA-oxidase, and polyphenol oxidase from peanutcells.Can. J. Bot. 55, 2630-2635 (1977).
  • 13.Gove J. P. and Hoyle M. C. The isozymic similarity of indoleaceticAcid oxidase to peroxidase in Birch and Horseradish. PlantPhysiol. 56, 684-687 (1975).
  • 14.Srivastava O. P., and Van Huystee R. B. Evidence for closeassociation of peroxidase, Polypenol oxidase, and IAA oxidaseisozyme of peanut suspension culture medium. Can. J. Bot. 51:2207-2215 (1973).
  • 15.Pilet, P. E; Lavanchy, P. and Sevhonkian, S. Interactions betweenperoxidase, polypenol oxidase and auxin oxidase. Physiol. Plant.23: 800-804 (1970).
  • 16.Davis B. J Disc electrophoresis II. method and application tohuman serum proteins (1964).
  • 17.Alcazar, M. D.,Egea, C.,Espin, A., Candela M. E. Peroxidaseisoenzymes in the defense response of Capsicum alnnuum toPhyophthora capsici. Physiol. Plant. 94: 736-742 (1995).
  • 18.Ye, X. S. Pan, S. O. and Kuc, J. Activity, isoenzyme pattern andcellular localization of peroxidase as related to systemic resistanceof tobacco to blue mold (Peronospora tabacina)and to tobaccomosaic virus. Phytopathology 80: 1295-1299 (1990).
  • 19.Hoyle, M. C. and Routley, D. G. Promotion of the IAA oxidase,Peroxidase complex enzyme in yellow birch. The Roya Soc. NewZeland, Wellington, pp 743-750 (1974).
  • 20.Lowry, O. H., Rosebrough, N. S. Farr A. L., and Randall, R. J.Protein measurements with folin-fenol reagent. J. Biol. Chem,193: 365 (1951).
  • 21.Guskow, A. V., Tikhomirov, I.A. and Polikarpova, F. Relationshipbetween peroxidases and root formation ability in stem cuttings ofclonal rootstock of apple. Physiologia Rastneii 7: 21-23 (1988).
  • 22.Haissig, B. E. Meristematic activity during adventitious roodprimordium development influences of endogenous auxin andapplied Giberrelic acid. Plant Physiol.: 49, 886-892 (1972