Bekir DOĞAN, Murat ÜNAL, Fevzi ÖZGÖKÇE, Esra MARTİN, Ayla KAYA

Türkiye Malcolmia, Strigosella, Zuvanda ve diğer yakın cinsler (Brassicaceae) arasındaki filogenetik akrabalık ilişkilerinin inter-simple sequence repeat çoğaltımı ile analizi

Malcolmia W.T.Aiton cinsi taksonomik olarak problemli bir cinstir ve son yıllarda yapılan çalışmalarla bazı türleri Strigosella Boiss. ve Zuvanda Dvorák cinslerine aktarılmıştır. Bu cinsin Türkiye’de üç türü doğal olarak yetişmektedir. Son yıllarda moleküler verilere göre yapılmış olan revizyon çalışmaları taksonların filogenetik ilişkilerini ve sistematik pozisyonlarını daha güvenilir ve net bir biçimde ortaya koymaktadır. Böylece fenotipik analizlerin aksine çevre şartlarından etkilenmeyen DNA tabanlı moleküler genetik analizlerle taksonomik problemi olan türlerin sorunları çözülmeye başlanmıştır. Bu çalışmada ISSR primerleri kullanılarak DNA’ların çoğaltılması yapılmış ve taksonlar arasındaki filogenetik ilişkilerin dendrogramı NTSYSpc 2.1 programı kullanılarak elde edilmiştir. Malcolmia cinsi içi ve diğer yakın cinsler arasındaki filogenetik akrabalıklar tespit edilmeye çalışılmıştır. Malcolmia chia ve M. flexuosa arasında çok yakın bir akrabalık ilişkisi tespit edilmiştir. Strigosella ve Zuvanda türlerinin bu iki türden filogenetik olarak ayrı olduğu belirlenmiştir. Malcolmia, Strigosella, Zuvanda, Leptaleum DC., Neotorularia Hedge & J.Léonard ve Sisymbrium L. cinsleri arasındaki filogenetik ilişkiler incelenmiştir. Malcolmia, Strigosella, Zuvanda, Leptaleum, Neotorularia ve Sisymbrium cinsleri filogenetik olarak dendrogramda ayrı kladlarda yer almıştır.

Anahtar Kelimeler:

Cruciferae Malcolmia, ISSR, filogeni, Türkiye

Phylogenetic relationships between Malcolmia, Strigosella, Zuvanda, and some closely related genera (Brassicaceae) from Turkey revealed by inter-simple sequence repeat amplification

The genus Malcolmia W.T.Aiton is taxonomically problematic, and some of its species have recently been transferred to the genera Strigosella Boiss. and Zuvanda Dvorák. Three species of this genus are native to Turkey. The revision studies based on molecular data, which were collected in the past few years, display the phylogenetic relations and the systematic positions of the taxa more reliably and apparently. Thus taxonomic problems of species are resolved through DNA-based molecular analyses, which are not affected by environmental factors compared to phenotypic studies. In the present study, the amplifications of the DNA fragments were carried out using ISSR primers, and the phylogenetic relationship among the taxa was revealed through a dendrogram produced as the outcome of the NTSYSpc 2.1 software. The infrageneric and intergeneric phylogenetic relationships among Malcolmia and other related genera are determined. A very close relationship was determined between Malcolmia chia and M. flexuosa. Strigosella and Zuvanda species were determined to be phylogenetically different from these 2 species. The phylogenetic relationships among the Malcolmia, Strigosella, Zuvanda, Leptaleum DC., Neotorularia Hedge & J.Léonard, and Sisymbrium L. taxa were investigated. The phylogenetic separation of Malcolmia, Strigosella, Zuvanda Leptaleum, Neotorularia, and Sisymbrium genera and their specimens constituted separate clades on the dendrogram.

Keywords:

Malcolmia ISSR, phylogeny, Turkey, Cruciferae,

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