Barbara KOŁODZIEJ, Sylwia OKOŃ, Aleksandra NUCIA, Tomasz OCIEPA, Katarzyna LUCHOWSKA, Danuta SUGIER, Reneta GEVRENOVA, Max HENRY

Morphological, chemical, and genetic diversity of Gypsophila L. (Caryophyllaceae) species and their potential use in the pharmaceutical industry

The aim of this study was to evaluate the morphological, chemical, and genetic similarity of species belonging to the genus Gypsophila L. The analysis included 7 Gypsophila species with potential use in the pharmaceutical industry for saponin production. In order to assess the variation of the morphological traits, a number of morphological characteristics have been determined (including the number of lateral roots, diameter and total root length, average plant height, number of shoots and their diameter, number of branches on the shoot, length and width of leaves, and fresh and air-dried weight of aerial and underground parts of plants), while prosaponin content was determined as the chemical trait. Gypsogenin 3-O-glucuronide and quillaic acid 3-O-glucuronide were the dominant prosaponins determined in the Gypsophila roots (amounting up to 13.5 mg g-1 dry extract in G. scorzonerifolia Ser. and 12.3 mg g-1 dry extract in G. acutifolia Spreng., respectively). Moreover, a variation analysis at the molecular level was carried out based on the RAPD and ISSR methods. The conducted analysis showed that the most distinct species at the morphological, chemical, and genetic levels were G. paniculata L. and G. oldhamiana Miq.

Keywords:

Gypsophila, biodiversity, molecular markers, saponins,

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Alam SMM, Siddika S, Haque ME, Islam MA, Mukherjee A, Sikdar B (2016). Genetic diversity of some upland and lowland rice cultivars in Bangladesh using RAPD, ISSR and SSR markers. Nucleus 59: 15-23.
Antkowiak W (2004). Yield of Gypsophila paniculata roots in dependance of different NPK fertilization. Roczniki Akademii Rolniczej w Poznaniu - CCCLX 38: 3-8 (in Polish with abstract in English).
Antkowiak W, Dyba S (2004). Effect of differentiated NPK fertilization on saponin content in roots of tall and spread gypsophyll ( Gypsophila paniculata L. and G. repens L.) in the second and third years of cultivation. Prace z Zakresu Nauk Rolniczych 97: 9-14.
Ataşlar E, Potoğlu Erkara İ, Tokur S (2009). Pollen morphology of some Gypsophila L. (Caryophyllaceae) species and its taxonomic value. Turk J Bot 33: 335-351.
Banerjee R, Chattopadhyay S, Saha AK (2016). Genetic diversity and relationship of mulberry genotypes revealed by RAPD and ISSR markers. Journal of Crop Improvement 30: 478-492.
Barkoudah YI (1962). A revision of Gypsophila , Bolanthus , Ankyropetalum and Phyrna . Wentia 9: 1-203.
Behera TK, Singh AK, Staub JE (2008). Comparative analysis of genetic diversity in Indian bitter gourd ( Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies. Sci Hortic-Amsterdam 115: 209-217.
Belaj A, Trujillo I, De La Rosa R, Rallo L, Giménez MJ (2001). Polymorphism and discrimination capacity of randomly amplified polymorphic markers in an olive germplasm bank. J Am Soc Hortic Sci 126: 64-71.
Brock J, Aboling S, Stelzer R, Esch E, Papenbrock J (2007). Genetic variation among different populations of Aster tripolium grown on naturally and anthropogenic salt-contaminated habitats: implications for conservation strategies. J Plant Res 120: 99- 112.
Calistri E, Buiatti M, Bogani P (2014). Characterization of Gypsophila species and commercial hybrids with nuclear whole-genome and cytoplasmic molecular markers. Plant Biosyst 150: 11-21.
Doyle JJ, Doyle JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
Falatoury AN, Assadi M, Ghahremaninejad F (2015). Taxonomic significance of indumentum in the genus Gypsophila L. (Caryophyllaceae). Nova Biologica Reperta 2: 91-102.
Fernandez M, Figueiras A, Benito C (2002). The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theor Appl Genet 104: 845-851.
Ferrão LFV, Caixeta ET, de Souza F, Zambolim EM, Cruz CD, Zambolim L, Sakiyama NS (2013). Comparative study of different molecular markers for classifying and establishing genetic relationships in Coffea canephora . Plant Syst Evol 299: 225-238.
Gevrenova R, Joubert O, Mandova T, Zaiou M, Chapleur Y, Henry M (2014). Cytotoxic effects of four Caryophyllaceae species extracts on macrophage cell lines. Pharm Biol 52: 919-925.
Gevrenova R, Stancheva T, Voynikov Y, Laurain-Mattar D, Henry M (2010). Root in vitro cultures of six Gypsophila species and their saponin contents. Enzyme Microb Tech 47: 97-104.
Greenberg AK, Donoghue MJ (2011). Molecular systematics and character evolution in Caryophyllaceae. Taxon 60: 1637-1652.
Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999). Molecular markers and their applications in wheat breeding. Plant Breeding 118: 369-390.
Hammer Ø, Harper DA, Ryan PD (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron 4: 5-7.
Hayashi T, Kakeya H, Konishi K (2009). Path analysis of interrelations of some components determining the number of florets per shoot in Gypsophila paniculata L. J Jpn Soc Hortic Sci 78: 330- 334.
Henry M (2005). Saponins and phylogeny: example of the “Gypsogenin group” saponins. Phytochem Rev 4: 89-94.
Henry M, Pauthe-Dayde D, Rochd M (1989). Extraction and high-performance liquid chromatographic determination of gypsogenin 3,O-glucuronide. J Chromatogr A 477: 413-419.
Henry M, Rochd M, Bennini B (1991). Biosynthesis and accumulation of saponins in Gypsophila paniculata . Phytochemistry 30: 1819-1821.
Hosokawa K, Minami M, Kawahara K, Nakamura I, Shibata T (2000). Discrimination among three species of medicinal Scutellaria plants using RAPD markers. Planta Med 66: 270-272.
Iankulov I (1974). Plant growth and changes in the amount and stability of root foam in Samokov 477 variety of Gypsophila paniculata L. during its growing season. Plant Sci. 11: 77-84.
ICH (2005). International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Text and Methodology Q2(R1). Geneva, Switzerland: International Council on Harmonisation.
Korkmaz M, Dogan NY (2015). Biogeographic pattern of genetic diversity detected by RAPD and ISSR analysis in Gypsophila (Caryophyllaceae) species from Turkey. Genet Mol Res 14: 8829-8838.
Korkmaz M, Özçelik H (2011). Economic importance of Gypsophila L., Ankyropetalum Fenzl and Saponaria L. (Caryophyllaceae) taxa of Turkey. Afr J Biotechnol 10: 9533-9541.
Kovach W (1999). MVSP – A Multivariate Statistical Package for Windows, Ver. 3.1. Pentraeth, UK: Kovach Computing Services.
Li R, Dao Z (2011). Molecular authentication of the traditional Tibetan medicinal plant, Meconopsis impedita . Afr J Biotechnol 10: 7493-7496.
Manole A, Banciu C, Indreica A (2015). Genetic diversity within a newly identified population of Adenophora liliifolia (L.) A.DC. in Romania: implications for conservation. Ann For Res 58: 1.
Martínez-Nieto MI, Segarra-Moragues JG, Merlo E, Martínez- Hernández F, Mota JF (2013). Genetic diversity, genetic structure and phylogeography of the Iberian endemic Gypsophila struthium (Caryophyllaceae) as revealed by AFLP and plastid DNA sequences: connecting habitat fragmentation and diversification. Biol J Linn Soc 173: 654-675.
Mohan M, Nair S, Bhagwat A, Krishna TG, Yano M, Bhatia CR, Sasaki T (1997). Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breeding 3: 87- 103.
Nei M, Li WH (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. P Natl Acad Sci USA 76: 5269-5273.
Okoń S, Paczos-Grzęda E, Łoboda M, Sugier D (2014). Identification of genetic diversity among Arnica montana L. genotypes using RAPD markers. Acta Sci Pol-Hortoru 13: 63-71.
Okoń S, Surmacz-Magdziak A, Paczos-Grzęda E (2013). Genetic diversity among cultivated and wild chamomile germplasm based on ISSR analysis. Acta Sci Pol-Hortoru 12: 43-50.
Prevost A, Wilkinson MJ (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98: 107-112.
Samal KC, Jena RC, Swain SS, Das BK, Chand PK (2012). Evaluation of genetic diversity among commercial cultivars, hybrids and local mango ( Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers. Euphytica 185: 195-213.
Santos E, Matos M, Silva P, Figueiras AM, Benito C, Pinto-Carnide O (2016). Molecular diversity and genetic relationships in Secale. Jpn J Genet 95: 273-81.
Saxonov SV, Senator SA, Rozenberg GS (2011). Conservation issues floristic diversity Volga Basin reference to the Red Book. Izvestiya Samarskogo Nauchnogo Centra RAN 13: 91-100.
Schulman AH (2007). Molecular markers to assess genetic diversity. Euphytica 158: 313-321.
Soodabeh S, Hoda F, Pantea S, Fahimeh M, Gholamreza A (2011). Genetic diversity and relationships detected by inter simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) analysis among Polygonum species growing in North of Iran. Afr J Biotechnol 10: 18981-18986.
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531-6535.
Yao S, Ma L, Luo JG, Wang JS, Kong LY (2010). New triterpenoid saponins from the roots of Gypsophila paniculata L. Helv Chim Acta 93: 361-374.
Ziętkiewicz E, Rafalski A, Labuda D (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.