Induction of embryogenic tissue from immature zygotic embryos in Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loundon) Businsky

Karaçam embriyogenik dokuları (ED), birbirini takip eden iki yıl boyunca haftalık olarak 16 farklı ağaçtan toplanan olgunlaşmamış henüz kotiledon gelişimi göstermeyen zigotik embriyolardan elde edildi. ED’lerin indüklemesi ve sonrasında çoğaltılması için, 13,6 μM 2,4-D ve 2,2 μM BAP ilave edilmiş Douglas-fir cotyledon revised (DCR) besiyeri kullanıldı. ED genel indükleme frekansları 2004 yılında %0,92, 2005 yılında ise %1,96 olarak gerçekleşti; farklı ağaçlar için değerler sıfır ile %7,32 arasında değişti. ED indüklemesinde kullanılan explantlar, 2004 ve 2005 yıllarında yapılan örneklemelerde sırasıyla %0,38’i ve %0,62’si çoğalmaya devam eden hücre hatlarına dönüştüler.

Pinus nigra subsp. nigra var. caramanica (Loundon) Businsky olgunlaşmamış zigotik embriyolarından embriyogenik dokuların indüklemesi

Embryogenic cultures (EC) of Anatolian black pine (Pinus nigra J.F.Arnold subsp. nigra var. caramanica (Loudon) Businsky) were initiated from immature pre-cotyledonary zygotic embryos sampled weekly from 16 different trees in 2 consecutive years. Douglas-fir cotyledon revised (DCR) medium supplemented with 13.6 μM of 2,4-D and 2.2 μM of BAP was used for initiation and maintenance of EC. Overall initiation frequencies of EC in the study were 0.92% in 2004 and 1.96% in 2005; tree values ranged from 0.0% to 7.32%. Overall, 0.38% and 0.62% of the initial explants were converted into established cell lines in 2004 and 2005, respectively.

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