In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.

A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf., a multipurpose native tree species highly adapted to the harsh environmental conditions of Kuwait, has been established using shoot tips and stem nodal segments as explants. The explants were cultured on Murashige and Skoog (MS) basal medium with and without growth regulators. The nodal segments and shoot tips isolated from the primary cultures were cultured on hormone-free MS media containing 100 mg/l myo-Inositol, 150 mg/l glutamine and 2.5% sucrose for plant growth and elongation. Shoots for multiplication were maintained on MS media with low concentrations of 6-benzylaminopurine (BA) and subcultured every 20 days. However, explants cultured in higher concentrations of cytokinin and auxin induced callus. Shoots transferred to the MS media containing 10 mg/l Indole-3-butric acid (IBA) were rooted. Rooted plantlets were transferred to sterile soil media for acclimatisation and field evaluation.

In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.

A simple and efficient protocol for the clonal micropropagation of Ziziphus spina-christi (L.) Desf., a multipurpose native tree species highly adapted to the harsh environmental conditions of Kuwait, has been established using shoot tips and stem nodal segments as explants. The explants were cultured on Murashige and Skoog (MS) basal medium with and without growth regulators. The nodal segments and shoot tips isolated from the primary cultures were cultured on hormone-free MS media containing 100 mg/l myo-Inositol, 150 mg/l glutamine and 2.5% sucrose for plant growth and elongation. Shoots for multiplication were maintained on MS media with low concentrations of 6-benzylaminopurine (BA) and subcultured every 20 days. However, explants cultured in higher concentrations of cytokinin and auxin induced callus. Shoots transferred to the MS media containing 10 mg/l Indole-3-butric acid (IBA) were rooted. Rooted plantlets were transferred to sterile soil media for acclimatisation and field evaluation.

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Bibtex @ { tbtkbotany141371, journal = {Turkish Journal of Botany}, issn = {1300-008X}, eissn = {1303-6106}, address = {}, publisher = {TÜBİTAK}, year = {2003}, volume = {27}, pages = {167 - 172}, doi = {}, title = {In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.}, key = {cite}, author = {Sudhersan, C. and Hussaın, J.} }
APA Sudhersan, C , Hussaın, J . (2003). In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf. . Turkish Journal of Botany , 27 (3) , 167-172 .
MLA Sudhersan, C , Hussaın, J . "In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf." . Turkish Journal of Botany 27 (2003 ): 167-172 <
Chicago Sudhersan, C , Hussaın, J . "In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.". Turkish Journal of Botany 27 (2003 ): 167-172
RIS TY - JOUR T1 - In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf. AU - C. Sudhersan , J. Hussaın Y1 - 2003 PY - 2003 N1 - DO - T2 - Turkish Journal of Botany JF - Journal JO - JOR SP - 167 EP - 172 VL - 27 IS - 3 SN - 1300-008X-1303-6106 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Botany In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf. %A C. Sudhersan , J. Hussaın %T In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf. %D 2003 %J Turkish Journal of Botany %P 1300-008X-1303-6106 %V 27 %N 3 %R %U
ISNAD Sudhersan, C. , Hussaın, J. . "In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.". Turkish Journal of Botany 27 / 3 (Mart 2003): 167-172 .
AMA Sudhersan C , Hussaın J . In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.. Turkish Journal of Botany. 2003; 27(3): 167-172.
Vancouver Sudhersan C , Hussaın J . In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.. Turkish Journal of Botany. 2003; 27(3): 167-172.
IEEE C. Sudhersan ve J. Hussaın , "In vitro Clonal Propagation of a Multipurpose Tree, Ziziphus spina-christi (L.) Desf.", Turkish Journal of Botany, c. 27, sayı. 3, ss. 167-172, Mar. 2003