Muhammad Adnan SHAHID, Rashad Mukhtar BALAL, Muhammad Aslam PERVEZ, Tahira ABBAS, Muhammad Anjum AQUEEL, Muhammad Mansoor JAVAID, Francisco GARCIA-SANCHEZ

Foliar spray of phyto-extracts supplemented with silicon: an efficacious strategy to alleviate the salinity-induced deleterious effects in pea (Pisum sativum L.)

A pot culture study was conducted to assess the ameliorative effect of silicon, Melia azadirachta leaf extract, and sugar beet root extract, each applied individually or in different combinations, on salinity-induced detrimental effects in pea (Pisum sativum L.). Salinity markedly inhibited the growth, various gas exchange attributes, total phenol contents, membrane stability index, and productivity. On the other hand, lipid peroxidation, electrolyte leakage, H2O2 content, antioxidant activities, and leaf free proline and glycinebetaine contents were significantly enhanced by salinity. However, exogenously applied Si and phyto-extracts markedly alleviated the salinity-induced drastic effects on growth, gas exchange attributes, and productivity. Both phyto-extracts supplemented with silicon gave highly salinity mitigating effects by markedly improving growth, gas exchange attributes, enzymatic activities, osmolytes, and yield. The phyto-extracts and Si suppressed lipid peroxidation, electrolyte leakage, and H2O2 content by strengthening the enzymatic and nonenzymatic (proline and glycinebetaine) antioxidant defense system. The phyto-extracts and Si application also checked the root/leaf sodium and chloride contents, but improved the silicon contents. Thus, it can be concluded that exogenous application of silicon in combination with phyto-extracts of M. Azadirachta and sugar beet is a highly effective ameliorative approach to alleviate salinity-induced hazardous effects in plants, especially in pea, grown under a saline regime.

Anahtar Kelimeler:

Salinity, silicon, Melia azadirachta indica, sugar beet, gas exchange, antioxidants

Foliar spray of phyto-extracts supplemented with silicon: an efficacious strategy to alleviate the salinity-induced deleterious effects in pea (Pisum sativum L.)

Keywords:

Salinity, silicon, Melia azadirachta indica, sugar beet, gas exchange, antioxidants,

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Abbas W, Ashraf M, Akram NA (2010). Alleviation of salt-induced adverse effects in eggplant (Solanum melongena L.) by glycinebetaine and sugarbeet extracts. Sci Hortic 125: 188–195.
Ahmad F, Rahmatullah, Aziz T, Maqsood MA, Tahir MA, Kanwal S (2007). Effect of silicon application on wheat (Triticum aestivum L.) growth under water deficiency stress. Emir J Food Agr 19: 1–7.
Ahmad ST, Haddad R (2011). Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress. Czech J Genet Plant Breed 47: 17–27.
Ahmed MA, Rao AS, Ahemad SR, Ibrahim M (2012). Phytochemical studies and antioxidant activity of Melia azedarach leaves by dpph scavenging assay. Int J Pharm Appl 3: 271–276.
Apel K, Hirt H (2004). Reactive oxygen species: metabolism oxidatives and signal transduction. Ann Rev Plant Physiol Plant Mol Biol 55: 373–399.
Arnon DI (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24: 1–15.
Ayumi T, Masumi H, Ryoichi T (2004). Chlorophyll metabolism and plant growth. Kagaku Seibutsu 42: 93–98.
Azuma R, Ito N, Nakayama N, Suwa R, Nguyen NT, Larrinaga- Mayoral JA, Esaka M, Fujiyama H, Saneoka H (2010). Fruits are more sensitive to salinity than leaves and stems in pepper plants (Capsicum annuum L.). Sci Hortic 125: 171–178.
Bates L, Waldren R, Teare I (1973). Rapid determination of free proline for water-stress studies. Plant Soil 39: 205–207.
Chance B, Maehly AC (1955). Assay of catalase and peroxidase. Method Enzymol 2: 764–775.
Dai W, Zhang KQ, Duan BW, Sun CX, Zheng KL, Cai R, Zhuang JY (2005). Rapid determination of silicon content in rice. Rice Sci 12: 145–147.
Genard H, Saos JL, Hillard J, Tremolieres A, Boucaud J (1991). Effect of salinity on lipid composition, glycinebetaine content and photosynthetic activity in chloroplasts of Suaeda maritima. Plant Physiol Bioch 29: 421–427.
Giannopolitis CN, Ries SK (1977). Superoxide dismutase I. Occurrence in higher plants. Plant Physiol 59: 309–314.
Gomez KA, Gomez AA (1984). Statistical Procedures for Agricultural Research, 2nd ed. New York, NY, USA: Wiley.
Grieve C, Grattan S (1983). Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 70: 303–307.
Heath RL, Packer L (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys 125: 189–198.
Hsu SY, Kao CH (2003). Differential effect of sorbitol and polyethylene glycol on antioxidant enzymes in rice leaves. Plant Growth Regul 39: 83–90.
Iqbal N, Nazar R, Khan MIR, Khan NA (2012). Variations in photosynthesis and growth of mustard cultivars: Role of ethylene sensitivity. Sci Hortic 135: 1–6.
Jain M, Mathur G, Koul S, Sarin NB (2001). Ameliorative effects of proline on salt induced lipid peroxidation in Cell lines of groundnut (Arachis hypogea L.) Plant Cell Rep 20: 483–468.
Julkenen-Titto R (1985). Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. Agr Food Chem 33: 213–217.
Kanechi M, Hikosaka Y, Uno Y (2013). Application of sugarbeet pure and crude extracts containing glycinebetaine affects root growth, yield, and photosynthesis of tomato grown during summer, Sci Hortic 152: 9–15.
Kaur G, Kumar S, Thakur P, Malik JA, Bhandhari K, Sharma KD, Nayyar H (2011). Involvement of proline in response of chickpea (Cicer arietinum L.) to chilling stress at reproductive stage. Sci Hortic 128: 174–181.
Khan MIR, Iqbal N, Masood A, Khan NA (2012). Variation in salt tolerance of wheat cultivars: role of glycinebetaine and ethylene. Pedosphere 22: 746–754.
Liu ZJ, Guo YK, Bai JG (2010). Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects ultra- structure in leaves of two cucumber ecotypes under osmotic stress. J Plant Growth Regul 29: 171–183.
Mack G, Hoffmann CM, Marlander B (2007). Nitrogen compounds in organs of two sugar beet genotypes (Beta vulgaris L.) during the season. Field Crop Res 102: 210–218.
Makela P (2004). Agro-industrial uses of glycinebetaine. Sugar Technol 6: 207–212.
Mateos-Naranjo E, Andrades-Moreno L, Davy AJ (2013). Silicon alleviates deleterious effects of high salinity on the halophytic grass Spartina densiflora. Plant Physiol Bioch 63: 115–121.
McCord JM (2000). The evolution of free radicals and oxidative stress. Am J Med 108: 652–659.
Moya LJ, Gomez-Cadenas A, Primo-Millo E, Talon M (2003). Chloride absorption in salt-sensitive Carrizo citrange and salt-tolerant Cleopatra mandarin citrus rootstocks is linked to water use. J Exp Bot 54: 825–833.
Nahak G, Sahu RK (2010). In vitro antioxidative acitivity of Azadirachta indica and Melia azedarach Leaves by DPPH scavenging assay. J Am Sci 6: 123–128.
Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867–880.
Neycee MA, Nematzadeh GHA, Dehestani A, Alavi M (2012). Assessment of antifungal effects of shoot extracts in chinaberry (Melia azedarach) against 5 phytopathogenic fungi. Int J Agr Crop Sci 4: 474–477.
Rachokarn S, Piyasaengthong N, Bullangpoti V (2008). Impact of botanical extracts derived from leaf extracts Melia azedarach L. (Meliaceae) and Amaranthus viridis L. (Amaranthaceae) on populations of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) and detoxification enzyme activities. Commun Agr Appl Biol Sci 73: 451–457.
Rao AS, Ahmed MF, Ibrahim M (2012). Hepatoprotective activity of Melia azedarach leaf extract against simvastatin induced hepatotoxicity in rats. J Appl Pharm Sci 2: 144–148.
Schutzendubel A, Polle A (2002). Plant responses to abiotic stresses: heavy metal induced oxidative stress and protection by mycorrhization. J Exp Bot 53: 1351–1365.
Shahid MA, Ashraf MY, Pervez MA, Ahmad R, Balal RM, Garcia- Sanchez F (2012). Impact of salt stress on concentrations of Na+, Cl- and organic solutes concentration in pea cultivars. Pakistan J Bot 45: 755–761.
Shahid MA, Balal RM, Pervez MA, Abbas T, Ashfaq M, Ghazanfar U, Afzal M, A. Rashid A, Garcia-Sanchez, Mattson NS (2012). Differential response of pea (Pisum sativum L.) genotypes to salt stress in relation to the growth, physiological attributes antioxidant activity and organic solutes. Aust J Crop Sci 6: 828–838.
Shen X, Zhou Y, Duan L, Li Z, Eneji AE, Li J (2010). Silicon effects on photosynthesis and antioxidant parameters of soybean seedlings under drought and ultraviolet-B radiation. J Plant Physiol 167: 1248–1252.
Shu S, Yuan L, Guo S, Sun J, Yuan Y (2013). Effects of exogenous spermine on chlorophyll fluorescence, antioxidant system and ultrastructure of chloroplasts in Cucumis sativus L. under salt stress. Plant Physiol Bioch 63: 209–216.
Smith IK, Vierheller TL, Thorne CA (1988). Assay of glutathione reductase in crude tissue homogenates using 5,5-dithiobis (2-nitrobenzoic acid). Ann Biochem 175: 408–413.
Sultana S, Akhtar N, Asif HM (2013). Phytochemical screening and antipyretic effects of hydro-methanol extract of Melia azedarach leaves in rabbits. Bangladesh J Pharmacol 8: 214– 217.
Suresh K, Deepa P, Harisaranraj R, Achudhan V (2008). Antimicrobial and phytochemical investigation of the leaves of Carica papaya L., Cynodon dactylon (L.) Pers., Euphorbia hirta L., Melia azedarach L. and Psidium guajava L. Ethnobot Leaflets 12: 1184–1191.
Sullivan CY, Ross W (1979). Selecting for drought and heat resistance in grain sorghum. Stress Physiol Crop Plant 1: 263–281.
Urbanek H, Kuzniak-Gebarowska E, Herka K (1991). Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase. Acta Physiol Plant 13: 43–50.
Zwart FJ, Slow S, Payne RJ, Lever M, George PM, Gerrard JA, Chambers ST (2003). Glycinebetaine and glycinebetaine analogues in common foods. Food Chem 83: 197–204.