Karim BAZIZ, Meriem BENAMARA-BELLAGHA, Fatima PUSTAHIJA, Spencer C. BROWN, Sonja SILJAK-YAKOVLEV, Nadra KHALFALLAH

First karyotype analysis, physical rDNA mapping, and genome size assessment in 4 North African Astragalus taxa (Fabaceae)

Four taxa of Algerian Astragalus L. were studied for their chromosome number, karyotype features, and genome size. Fluorochrome banding was done for detection of GC-rich DNA regions, fluorescence in situ hybridization (FISH) for physical mapping of 35S and 5S rRNA genes, and flow cytometry for nuclear DNA content. All the taxa present the same chromosome number (2n = 2x = 16) and single 35S and 5S rDNA loci, but their distributions on chromosomes are different. The GC heterochromatin pattern was different among studied taxa and an unusually high number of chromomycin-positive bands were observed in A. pseudotrigonus Batt. & Trab. The genome size differed between species, ranging from 2C = 1.39 pg in A. cruciatus Link. to 2C = 2.71 pg in A. armatus subsp. tragacanthoides (Desf.) Maire. No difference in nuclear DNA amount was detected between the 2 subspecies of A. armatus. Although Astragalus is a large genus comprising some 3000 species, such morphometric and molecular cytogenetic karyotype analyses, with genome sizes, are particularly scarce therein. Therefore, published genome sizes have also been compiled into one table.

Anahtar Kelimeler:

Astragalus, DNA content, fluorescence in situ hybridization, fluorochrome banding, rDNA mapping

First karyotype analysis, physical rDNA mapping, and genome size assessment in 4 North African Astragalus taxa (Fabaceae)

Keywords:

Astragalus, DNA content, fluorescence in situ hybridization, fluorochrome banding, rDNA mapping,

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Abdel Samad F, Baumel A, Juin M, Pavon D, Siljak-Yakovlev S, Médail F, Bou Dagher Kharrat M (2014). Phylogenetic diversity and genome sizes of Astragalus (Fabaceae) in the Lebanon biogeographical crossroad. Plant Syst Evol 300: 819–830.
Adams KL, Palmer JD (2003). Evolution of mitochondrial gene content: gene loss and transfer to the nucleus. Mol Phylogenet Evol 29: 380–395.
Agerer-Kirchhoff C (1976). Revision von Astragalus L. sect. Astragalus (Leguminosae). Boissiera 25: 1–197 (in German).
Arano H, Saito H (1980). Cytological studies in family Umbelliferae 5. Karyotypes of seven species in subtribe Seselinae. La Kromosomo 2: 471–480.
Badr A, Sharawy SM (2007). Karyotype analysis and systematic relationships in the Egyptian Astragalus L. (Fabaceae). Int J Bot 3: 147–159.
Bogunic F, Muratovic E, Ballian D, Siljak-Yakovlev S, Brown S (2007). Genome size stability of five subspecies of Pinus nigra Arnold s.l. Env Exp Bot 59: 354–360.
Bou Dagher-Kharrat M, Grenier G, Bariteau M, Brown SC, Siljak- Yakovlev S, Savouré A (2001). Karyotype analysis reveals interspeciŞc differentiation in the genus Cedrus despite genome size and base composition constancy. Theor Appl Genet 103: 846–854.
Bou Dagher-Kharrat M, Siljak-Yakovlev S, Abdel-Samad N, Douaihy BC, Abdel-Samad F, Bourge M, Brown SC (2013). Nuclear DNA C-values for biodiversity screening: case of the Lebanese flora. Plant Biosyst 147: 1228–1237.
Deutsch M, Long M (1999). Intron-exon structure of eukaryotic model organisms. Nucleic Acids Res 27: 3219–3228.
Doležel J, Bartoš J, Voglmayr H, Greilhuber J (2003). Nuclear DNA content and genome size of trout and human. Cytometry 51: 127–128.
Geber G, Schweizer D (1987). Cytochemical heterochromatin differentiation in Sinapis alba (Cruciferae) using a simple air drying technique for producing chromosome spreads. Plant Syst Evol 158: 97–106.
Greilhuber J, Speta F (1976). C-banded karyotypes in the Scilla hohenackeri group, S. persica, and Puschkinia (Liliaceae). Plant Syst Evol 126: 149–188.
Hamon P, Siljak-Yakovlev S, Srisuwan S, Robin O, Poncet V, Hamon S, De Kochko A (2009). Physical mapping of rDNA and heterochromatin in chromosomes of 16 Coffea species: a revised view of species differentiation. Chrom Res 17: 291–304.
Heslop-Harrison JS, Schwarzacher T, Anamthawat-Jónsson K, Leitch AR, Shi M, Leitch IJ (1991). In situ hybridization with automated chromosome denaturation. Technique 3: 109–116.
Kazem Y, Houshmand S, Zamani Dadane G (2010). Karyotype analysis of Astragalus effusus Bunge (Fabaceae). Caryologia 63: 257–261.
Kazempour Osaloo S, Maassoumi AA, Murakami N (2005). Molecular systematics of the Old World Astragalus (Fabaceae) as inferred from nrDNA ITS sequence data. Brittonia 57: 367– 381.
Kim SY, Choi HW, Kim CS, Sung JS, Lee J, Bang JW (2006). Cytogenetics analyses of Astragalus species. Korean Journal of Medicinal Crop Science 14: 250–254.
Leitch IJ, Chase MW, Bennett MD (1998). Phylogenetic analysis of DNA C-values provides evidence for a small ancestral genome size in ﬂowering plants. Ann Bot 82: 85–94.
Levan A, Fredga K, Sandberg AA (1964). Nomenclature for centromere position in chromosomes. Hereditas 52: 201–220.
Lysák MA, Cíhalíková J, Kubaláková M, Simková H, Künzel G, Doležel J (1999). Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.). Chromosome Res 7: 431–444.
Ma XH, Qin RL, Xing WB (1984). Chromosome observations of some medical plants in Xinjiang. Acta Phytotaxon Sin 22: 243– 249 (in Chinese with English abstract).
Maluszynska J, Heslop-Harrison JS (1991). Localization of tandemly repeated DNA sequences in Arabidopsis thaliana. Plant J 1: 159–166.
Manandhar L, Sakya SR (2004). Cytotaxonomic studies in two species of Astragalus. J Cytol Genet 5: 13–20.
Marie M, Brown SC (1993). A cytometric exercise in plant DNA histograms, with 2C values for 70 species. Biol Cell 78: 41–51.
Martin E, Duran A, Dinç M, Erişen S (2008). Karyotype analyses of four Astragalus L. (Fabaceae) species from Turkey. Phytologia 90: 147–159.
Morgante M, Hanafey M, Powell W (2002). Microsatellites are preferentially associated with non-repetitive DNA in plant genomes. Nat Genet 30: 194–200.
Mortreau E, Siljak-Yakovlev S, Cerbah M, Brown SC, Bertrand H, Lambert C (2010). Cytogenetic characterization of Hydrangea involucrata Sieb. and H. aspera D. Don complex (Hydrangeaceae): genetic, evolutional, and taxonomic implications. Tree Genet Genomes 6: 137–148.
Muratović E, Robin O, Bogunić F, Šoljan D, Siljak-Yakovlev S (2010). Karyotype evolution and speciation of European lilies from Lilium sect. Liriotypus. Taxon 59: 165–175.
Piegu B, Guyot R, Picault N, Roulin A, Sanyal A, Kim H, Collura K, Brar DS, Jackson S, Wing RA et al. (2006). Doubling genome size without polyploidization: Dynamics of retrotransposition- driven genomic expansions in Oryza australiensis, a wild relative of rice. Genome Res 16: 1262–1269.
Podlech D (1986). Taxonomic and phytogeographical problems in Astragalus of the Old World and South West Asia. P Roy Soc Edinb B 89: 37–43.
Podlech D (1994). Revision der altweltlichen anuellen Arten der Gattung Astragalus L. (Leguminosae). Sendtnera 2: 39–170 (in German).
Pustahija F, Brown SC, Bogunić F, Bašić N, Muratović E, Ollier S, Hidalgo O, Bourge M, Stevanović V, Siljak-Yakovlev S (2013). Small genomes dominate in plants growing on serpentine soils in West Balkans, an exhaustive study of 8 habitats covering 308 taxa. Plant Soil 373: 427–453.
Quézel P, Santa S (1962). Nouvelle flore de l’Algérie et des régions désertiques méridionales (Tome 1). Paris, France: Editions du CNRS (in French).
Scherson RA, Vidal R, Sanderson MJ (2008). Phylogeny, biogeography and rates of diversification of New World Astragalus (Leguminosae) with an emphasis on South American radiations. Am J Bot 95: 1030–1039.
Schubert I, Wobus U (1985). In situ hybridization confirms jumping nucleolus organizing regions in Allium. Chromosoma 92: 143– 148.
Schweizer D (1976). Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma 58: 307–324.
Senn HA (1938). Chromosome number relationships in the Leguminosae. Bibliographia Genetica 12: 175–336.
Siljak-Yakovlev S, Cerbah M, Coulaud J, Stoian V, Brown SC, Jelenic S, Papes D (2002). Nuclear DNA content, base composition, heterochromatin and rDNA in Picea omorika and Picea abies. Theor Appl Gene 104: 505–512.
Siljak-Yakovlev S, Pustahija F, Šolić EM, Bogunić F, Muratović E, Bašić N, Catrice O, Brown SC (2010). Towards a genome size and chromosome number database of Balkan Flora: C-values in 343 taxa with novel values for 242. Adv Sci Lett 3: 190–213.
Srebniak M, Rasmussen O, Maluszynska J (2002). Cytogenetic analysis of an asymmetric potato hybrid. J Appl Genet 43:19– 31.
Stebbins GL (1971). Chromosomal Evolution in Higher Plants. London, UK: Edward Arnold.
Temsch EM, Temsch W, Ehrendorfer-Schratt L, Greilhuber J (2010). Heavy metal pollution, selection, and genome size: the species of the Žerjav Study revisited with flow cytometry. Journal of Botany 2010: 596542.
Tietz S (1988). Revision von Astragalus L. section Campylanthus