Development of a highly responsive leaf-based regeneration system for Peperomia species

Several species in the genus Peperomia Ruiz & Pav. have giant chloroplasts in the palisade parenchyma of their leaves. Because of this unique feature, Peperomia metallica L. Linden & Rodigas has become a valuable model plant for studying plastid biology. However, the use of Peperomia for molecular studies has been limited by the lack of efficient regeneration and transformation protocols. In this work, we aimed to develop a reliable regeneration system for Peperomia from leaf explants. Using MS medium complemented with different combinations of phytohormones, a highly efficient in vitro regeneration protocol was developed for P. metallica and P. peduncularis, another species with giant chloroplasts. Regeneration occurs through direct organogenesis from leaf discs at a very high frequency. Our protocol can provide a reliable tissue culture system for future Peperomia genetic transformation experiments.

Anahtar Kelimeler:

Key words: Peperomia, tissue culture, giant chloroplast, regeneration, gibberellic acid

Development of a highly responsive leaf-based regeneration system for Peperomia species

Keywords:

Key words: Peperomia, tissue culture, giant chloroplast, regeneration, gibberellic acid,

PDF

___

Bartels F (1965). Die Plastiden von Peperomia metallica (Plastidenzählungen). Z Bot 52: 572-599.
Bulychev AA, Andrianov VK, Kurella GA & Litvin FF (1972). Micro-electrode measurements of the transmembrane potential of chloroplasts and its photoinduced changes. Nature 236: 175-177.
Gazzarrini S & McCourt P (2003). Cross-talk in plant hormone signalling: what Arabidopsis mutants are telling us. Ann Bot 91: 605-612.
Hartig K & Beck E (2006). Crosstalk between auxin, cytokinins, and sugars in the plant cell cycle. Plant Biol 8: 389-396.
Klimaszewska K (1979). The regenerative potential of two species of Peperomia in tissue culture and biological activity of endogenous growth regulators. Acta Hort 91: 281-286.
Kukulczanka K, Klimaszewska K & Pluta H (1977). Regeneration of entire plants of Peperomia scandens Ruiz. from different parts of leaves in vitro. Acta Hort 78: 365-371.
Lakshmanan P, Geijskes RJ, Wang L, Elliott A, Grof CP, Berding N & Smith GR (2006). Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture. Plant Cell Rep 25: 1007-1015.
Lincy A & Sasikumar B (2010). Enhanced adventitious shoot regeneration from aerial stem explants of ginger using TDZ and its histological studies. Turk J Bot 34: 21-29.
Murashige T & Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497.
Neumann D (1973). Zur Ultrastruktur der Riesenplastiden aus dem Palisadenparenchym von Peperomia metallica Lind et Rodig. Protoplasma 77: 467-471.
Riou-Khamlichi C, Huntley R, Jacqmard A & Murray JA (1999). Cytokinin activation of Arabidopsis cell division through a D-type cyclin. Science 283: 1541-1544.
Sahai A, Shahzad A & Anis M (2010). High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turk J Bot 34: 11-20.
Schürhoff P (1908). Ozellen und Lichtkondensatoren bei einigen Peperomien. Beih Bot Zbl (Abt I) 23: 14-26.
Sheridan WF (1975). Tissue culture of maize. I. Callus induction and growth. Physiol Plant 33: 151-156.
Singh DP, Jermakow AM & Swain SM (2002). Gibberellins are required for seed development and pollen tube growth in Arabidopsis. Plant Cell 14: 3133-3147.
Srejović V & Nešković M (1985). Effect of gibberellic acid on organogenesis in buckwheat tissue culture. Biol Plant 27: 432-437.
Thorpe T & Meier D (1973). Effects of gibberellic acid and abscisic acid on shoot formation in tobacco callus cultures. Physiol Plant 29: 121-124.
Wanke S, Samain MS, Vanderschaeve L, Mathieu G, Goetghebeur P & Neinhuis C (2006). Phylogeny of the genus Peperomia (Piperaceae) inferred from the trnK/matK region (cpDNA). Plant Biol 8: 93-102.