cpDNA supports the identification of the major lineages of American Blechnum (Blechnaceae, Polypodiopsida) established by morphology
Blechnaceae is an important leptosporangiate family (9-10 genera, about 250 species). It is monophyletic and distributed mainly in tropical America and Australasia. Among the species 80% belong to Blechnum, a genus with a very complex taxonomy and uncertain internal relationships. In terms of American diversity, the results of morphological studies have arrived at 8 informal groups. Molecular works on this genus are scarce, and there is no information for the majority of American species. The main objective of this work was to evaluate whether the groups proposed to organise the diversity of American of Blechnum are consistent with a molecular analysis. We sequenced 2 chloroplastic regions from species representing all of the groups. In our molecular analysis most of the informal groups were maintained as well supported clades. Only 2 species, B. brasiliense and B. spicant, appear to be isolated from their alleged relatives. Combining our molecular results with previous morphological knowledge, we propose the recognition of 4 lineages: 1) B. serrulatum , 2) B. spicant, and 3) core Blechnum, which represents a large clade that can be divided into core Blechnum I (arborescent species, cordatoids, and B. brasiliense) and core Blechnum II (epiphytic species and herbaceous terrestrials, both monomorphic and dimorphic groups).
Anahtar Kelimeler:
Key words: America, Blechnum, chloroplast DNA, morphology
cpDNA supports the identification of the major lineages of American Blechnum (Blechnaceae, Polypodiopsida) established by morphology
Blechnaceae is an important leptosporangiate family (9-10 genera, about 250 species). It is monophyletic and distributed mainly in tropical America and Australasia. Among the species 80% belong to Blechnum, a genus with a very complex taxonomy and uncertain internal relationships. In terms of American diversity, the results of morphological studies have arrived at 8 informal groups. Molecular works on this genus are scarce, and there is no information for the majority of American species. The main objective of this work was to evaluate whether the groups proposed to organise the diversity of American of Blechnum are consistent with a molecular analysis. We sequenced 2 chloroplastic regions from species representing all of the groups. In our molecular analysis most of the informal groups were maintained as well supported clades. Only 2 species, B. brasiliense and B. spicant, appear to be isolated from their alleged relatives. Combining our molecular results with previous morphological knowledge, we propose the recognition of 4 lineages: 1) B. serrulatum , 2) B. spicant, and 3) core Blechnum, which represents a large clade that can be divided into core Blechnum I (arborescent species, cordatoids, and B. brasiliense) and core Blechnum II (epiphytic species and herbaceous terrestrials, both monomorphic and dimorphic groups).
Keywords:
Key words: America, Blechnum, chloroplast DNA, morphology,
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- Aydın O, Coşkunçelebi K, Gültepe M & Güzel ME (2013). A contribution to taxonomy of Centaurea including Psephellus (Asteraceae) based on anatomical and molecular data. Turkish Journal of Botany 37: 419–427.
- Coşkunçelebi K, Terzioğlu S, Karaköşe M & Güzel ME (2012). Contributions to the description and molecular properties of Erodium hendrikii Alpinar (Geraniaceae), endemic to Turkey. Turkish Journal of Botany 36: 455–461.
- Cranfill R (2001). Phylogenetic Studies in the Polypodiales (Pteridophyta) with an Emphasis on the Family Blechnaceae. Berkeley, CA: University of California.
- Cranfill R, Nakahira Y & Kato M (2001). A molecular phylogeny of Blechnaceae inferred from three plastid gene sequences. Fifth International Flora Malesiana Symposium Abstract.
- Cranfill R & Kato M (2003). Phylogenetics, biogeography, and classification of the woodwardioid ferns (Blechnaceae). In: Chandra S & Srivastava M (eds.) Pteridology in the New Millennium. Netherlands: Kluwer Academic Publishers.
- Darriba D, Taboada G, Doallo R & Posada D (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772–772.
- Dittrich V (2005). Estudos taxonômicos no gênero Blechnum L. (Pteridophyta-Blechnaceae) para as regiões Sudeste e Sul do Brasil. Universidad Estadual Paulista Julio de Mesquita Filho, Brasil.
- Drummond A, Ashton B, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, StonesHavas S, Sturrock S, Thierer T & Wilson A (2011). Geneious Website http://www.geneious.com [accessed 30 November 2010].
- Durán M (1997). Estudios morfológicos, taxonómicos y biosistemáticos en el género Blechnum (Blechnaceae– Pteridophyta). Universidad Nacional de Córdoba, Córdoba, Argentina.
- Gabriel y Galán JM, Passarelli LM, Prada C & Rolleri CH (2008). Sporophyte morphology and gametophyte development of the fern Blechnum sprucei (Pteridophyta: Blechnaceae). Revista de Biologia Tropical 56: 2027–2040.
- Gabriel y Galán JM, Prada C, Rolleri CH, Lahoz-Beltrá R & MartínezCalvo C (2011). A biometrical study of stomata in Blechnum species (Blechnaceae) with some taxonomic and ecological implications for the ferns. Revista de Biología Tropical 59: 403–415.
- Giudice GE, Luna ML, Carrion C & de La Sota ER (2008). Revision of the genus Salpichlaena J. Sm. American Fern Journal 98: 49–60.
- Kramer K, Chambers T & Hennipman E (1990). Blechnaceae. In: Kramer K & Green P (eds.) The Families and Genera of Vascular Plants: I. Pteridophytes and Gymnosperms. Berlin: Springer.
- Kuo L-Y, Li F-W, Chiou W-L & Wang C-N (2011). First insights into fern matK phylogeny. Molecular Phylogenetics and Evolution 59: 556–566.
- Murillo M (1968). Blechnum subgénero Blechnum en Sur América, con especial referencia a las especies de Colombia. Nova Hedwigia 16: 329–366.
- Nagalingum N, Schneider H & Pryer K (2007). Molecular phylogenetic relationships and morphological evolution in the heterosporous fern genus Marsilea. Systematic Botany 32: 16–25.
- Passarelli L (2007). Estudios esporales en especies del grupo Blechnum penna-marina (Blechnaceae-Pteridophyta). Acta Botanica Malacitana 32: 1–19.
- Passarelli L, Gabriel y Galán JM, Prada C & Rolleri CH (2010). Spore morphology and ornamentation in the genus Blechnum (Blechnaceae, Pteridophyta). Grana 49: 243–262.
- Prada C, Rolleri C & Passarelli L (2007). Morfología, caracterización y distribución geográfica de Blechnum cordatum (BlechnaceaePteridophyta). Acta Botanica Malacitana 33: 29–46.
- Ramos Giacosa JP (2008) Revisión sistemática, análisis cladístico y biogeográfico de la sección Lomariocycas (J. Sm.) C. V. Morton del género Blechnum L. (Blechnaceae, Pteridophyta) en
- América. Universidad Nacional de La Plata, La Plata, Argentina. Rolleri C & Prada C (2006a). Catálogo comentado de las especies de Blechnum (Blechnaceae-Pteridophyta) de Mesoamérica y Sudamérica. Anales del Jardin Botanico de Madrid 63: 67–106.
- Rolleri C & Prada C (2006b). Revisión de los grupos de especies del género Blechnum (Blechnaceae- Pteridophyta): el grupo B. penna-marina. Acta Botanica Malacitana 31: 7–50.
- Rolleri C, Prada C & Passarelli L (2008). Estudios morfológicos y taxonómicos en Blechnum (Blechnaceae-Pteridophyta): Blechnum tabulare y B. magellanicum. Anales del Jardin Botanico de Madrid 65: 179–195.
- Rolleri C, Prada C, Gabriel y Galán JM, Passarelli LM & Ciciarelli MM (2010). Morphology of the sporophyte and gametophyte of the swamp fern, Blechnum serrulatum (Blechnaceae, Pteridophyta).
- Australian Journal of Botany 58: 508–518. Rolleri C, Prada C, Passarelli L & Gabriel y Galán JM (2012a). Revisión de dos especies de Blechnum de las regiones montañosas tropicales e intertropicales de Centroamérica y Sudamérica. Actes du Colloque Les Fougères d’Alsace, d’Europe et du Monde 1: 149–161.
- Rolleri C, Prada C, Passarelli L, Gabriel y Galán JM & Ciciarelli MM (2012b). Revisión de especies monomórficas y subdimórficas del género Blechnum (Blechnaceae-Polypodiophyta). Botanica Complutensis 36: 51–77.
- Rolleri C, Prada C, Gabriel y Galán JM & Passarelli L (2013). Caracterización y revisión de ocho especies arborescentes del género Blechnum (Blechnaceae-Polypodiophyta). Revista de Biologia Tropical 61: 377–408.
- Rothfels CJ, Larsson A, Kuo L-Y, Korall P, Chiou W-L & Pryer KM (2012a). Overcoming deep roots, fast rates, and short internodes to resolve the ancient rapid radiation of eupolypod II ferns. Systematic Biology 61: 490–509.
- Rothfels CJ, Sundue MA, Kuo L-Y, Larsson A, Kato M, Schuettpelz E & Pryer KM (2012b). A revised family-level classification for eupolypod II ferns (Polypodiidae: Polypodiales). Taxon 61: 515–533.
- Schuettpelz E & Pryer KM (2007). Fern phylogeny inferred from 400 leptosporangiate species and three plastid genes. Taxon 56: 1037–1050.
- Shepherd LD, Perrie LR, Parris BS & Brownsey PJ (2007). A molecular phylogeny for the New Zealand Blechnaceae ferns from analyses of chloroplast trnL-trnF DNA sequences. New Zealand Journal of Botany 45: 67–80.
- Simmons MP & Ochoterena H (2000). Gaps as characters in sequence-based phylogenetic analyses. Systematic Biology 49: 369–81.
- Smith AR, Pryer KM, Schuettpelz E, Korall P, Schneider H & Wolf PG (2006). A classification for extant ferns. Taxon 55: 705–731.
- Taberlet P, Gielly L, Patou G & Bouvet J (1991). Universal primers for amplification of three noncoding regions of chloroplast DNA. Plant Molecular Biology 17: 1105–1109.
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M & Kumar S (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution 28: 2731–2739.
- Tryon AF & Lugardon B (1991). Spores of the Pteridophyta. New York: Springer.
- Tryon RM & Tryon AF (1982). Ferns and Allied Plants with Special Reference to Tropical America. New York: Springer.
- Tryon RM & Stolze RG (1993). Pteridophyta of Peru. Part. V: Aspleniaceae-Polypodiaceae. Fieldiana Botany 32: 54–70.
- ISSN: 1300-008X
- Yayın Aralığı: Yılda 6 Sayı
- Yayıncı: TÜBİTAK
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