Mehmet Cengiz BALOĞLU, Musa KAVAS, Gülsüm AYDIN

Antioxidative and physiological responses of two sunflower (Helianthus annuus) cultivars under PEG-mediated drought stress

Drought stress is one of the most important yield-reducing factors in crop production. Sunflower, an oilseed crop, is severely affected by abiotic stress. In this study, 2 sunflower cultivars (Musala and Aydın) were evaluated in terms of various biochemical and physiological responses under 2 different polyethylene glycol-mediated drought stress conditions. Stress-determining parameters such as malondialdehyde (MDA), hydrogen peroxide (H2O2), and proline contents were determined. Both cultivars showed similar responses at osmotic potentials of both -0.4 and -0.8 MPa. Aydın was less affected than Musala under these stress conditions. MDA, H2O2, and proline levels were similar at both -0.4 and -0.8 MPa osmotic potentials in the 2 different cultivars. The 2 cultivars differed significantly in ascorbate peroxidase and catalase enzyme activities, which were more prominent in Aydın for both stress levels. However, glutathione reductase activity did not appear to be an essential part of the antioxidative system in either of the cultivars. Engineering antioxidative enzyme levels might provide a potential mechanism to cope with drought stress in sunflower.

Anahtar Kelimeler:

Key words: Helianthus annuus, drought stress, antioxidant enzymes, proline

Antioxidative and physiological responses of two sunflower (Helianthus annuus) cultivars under PEG-mediated drought stress

Keywords:

Key words: Helianthus annuus, drought stress, antioxidant enzymes, proline,

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Albert A & Schneiter ED (1997). Sunflower Technology and Production. Madison, Wisconsin: The American Society of Agronomy.
Alscher RG (1989). Biosynthesis and antioxidant properties of glutathione in plants. Physiologia Plantarum 77: 457-464.
Arora A, Sairam RK & Srivastava GC (2002). Oxidative stress and antioxidative systems in plants. Current Science 82: 1227-1238.
Ashraf M & Foolad MR (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59: 206-216.
Bates LS, Waldren RP & Teare ID (1973). Rapid determination of free proline for water-stress studies. Plant Soil 39: 205-207.
Bernt E & Bergmeyer HU (1974). Methods of Enzymatic Analysis. New York: Academic Press.
Bowler C, Montagu MV & Inze D (1992). Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology 43: 83-116.
Bradford MM (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248-254.
Bray EA, Bailey-Serres J & Weretilnyk E (2000). Responses to abiotic stresses. In: Gruissem W, Buchannan B & Jones R (eds.) Biochemistry and Molecular Biology of Plants, pp. 1158-1249. Rockville, Maryland: American Society of Plant Physiologists.
Camacho Barron M & Gonzalez de Mejia EG (1998). Comparative study of enzymes related to proline metabolism in tepary bean (Phaseolus acutifolius) and common bean (Phaseolus vulgaris) under drought and irrigated conditions, and various urea concentrations. Plant Foods for Human Nutrition 52: 119-132.
Celikkol Akcay U, Ercan O, Kavas M, Yildiz L, Yilmaz C, Oktem HA & Yucel M (2010). Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings. Plant Growth Regulation 61: 21-28.
Chance B & Maehly AC (1995). Assay of catalases and peroxidases. Methods in Enzymology 2: 764-817.
Chugh V, Kaur N & Gupta AK (2011). Evaluation of oxidative stress tolerance in maize (Zea mays L.) seedlings in response to drought. Indian Journal of Biochemistry & Biophysics 48: 47-53.
Connor DJ & Hall AJ (1997). Sunflower physiology. In: Schneiter AA (ed.) Sunflower Science and Technology, pp. 113-182. Madison, Wisconsin: The American Society of Agronomy.
Cruz de Carvalho MH (2008). Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signal Behavior 3: 156-165.
Fadzilla NM, Finch RP & Burdon RH (1997). Salinity, oxidative stress and antioxidant responses in shoot cultures of rice. Journal of Experimental Botany 48: 325-331.
Farooq M, Wahid A, Kobayashi N, Fujita D & Basra SMA (2009). Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29: 185-212.
Fridovich I (1986). Biological effects of superoxide radical. Archives of Biochemistry and Biophysics 247: 1-11.
Ghane SG, Lokhande VH & Nikam TD (2012). Differential growth, physiological and biochemical responses of niger (Guizotia abyssinica Cass.) cultivars to water-deficit (drought) stress. Acta Physiologiae Plantarum 34: 215-225.
Halliwell B (1987). Oxidative damage, lipid peroxidation, and antioxidant protection in chloroplasts. Chemistry and Physics of Lipids 44: 327-340.
Hoagland DR & Arnon DI (1950). The water-culture method for growing plants without soil. California Agricultural Experiment Station Circular 347: 1-32.
Kang SZ & Zhang JH (1997). Hydraulic conductivities in soil-root system and relative importance at different soil water potential and temperature. Transactions of Chinese Society of Agricultural Engineers 13: 76-81.
Knowles PF (1988). Recent advances in oil crops breeding. In: Applewhite TH (ed.) Proceedings of the World Conference on Biotechnology for the Fats and Oil Industry, pp. 35-38. Urbana, Illinois: American Oil Chemists Society.
Leon AJ, Andrade FH & Lee M (2003). Genetic analysis of seed- oil concentrations across generations and environments in sunflower (Helianthus annuus L.). Crop Science 43: 135-140.
Liu X & Baird WV (2003). Differential expression of genes regulated in response to drought or salinity stress in sunflower. Crop Science 43: 678-687.
Lopez-Pereira M, Trapani N & Sadras V (2000). Genetic improvement of sunflower in Argentina between 1930 and 1995. III. Dry matter partitioning and achene composition. Field Crops Research 67: 215-221.
Makbul S, Güler NS, Durmuş N & Güven S (2011). Changes in anatomical and physiological parameters of soybean under drought stress. Turkish Journal of Botany 35: 369-377.
Manivannan P, Abdul Jaleel C, Sankar B, Kishorekumar A, Somasundaram R, Lakshmanan GMA & Panneerselvam R (2007). Growth, biochemical modifications and proline metabolism in Helianthus annuus L. as induced by drought stress. Colloids and Surfaces B: Biointerfaces 59: 141-149.
Mazahery-Laghab H, Nouri F & Abianeh HZ (2003). Effects of the reduction of drought stress using supplementary irrigation for sunflower (Helianthus annuus L.) in dry farming conditions. Pajouhesh va Sazandegi in Agronomy and Horticulture 59: 81- 86.
Miller JF (1995). Inheritance of salt tolerance in sunflower. Helia 18: 9-16.
Mittler R (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410.
Monotti M (2004). Growing non-food sunflower in dry land conditions. The Italian Journal of Agronomy 8: 3-8.
Ohkawa H, Ohishi N & Yagi Y (1979). Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Analytical Biochemistry 95: 351-358.
Perez-Lopez U, Robredo A, Lacuesta M, Sgherri C, Munoz-Rueda A, Navari-Izzoc F & Mena-Petite A (2009). The oxidative stress caused by salinity in two barley cultivars is mitigated by elevated CO2. Physiologia Plantarum 135: 29-42.
Rabinowitch HD & Fridovic I (1983). Superoxide radical, superoxide dismutase and oxygen toxicity in plants. Photochemistry and Photobiology Sciences 188: 206-213.
Rauf S (2008). Breeding sunflower (Helianthus annuus L.) for drought tolerance. Communications in Biometry and Crop Science 3: 29-44.
Satoh K & Murata N (1998). Stress Responses of Photosynthetic Organisms: Molecular Mechanisms and Molecular Regulations. Amsterdam: Elsevier.
Sgherri CLM, Ligini B, Puliga S & Navari-Izzo F (1994). Antioxidant system in Sporobolus stapŞanus: changes in response to desiccation and rehydration. Phytochemistry 35: 561-565.
Sgherri CLM, Pinzino C & Navari-Izzo F (1996). Sunflower seedlings subjected to increasing stress by water deficit: changes in O2 production related to the composition of thylakoid membranes. Plant Physiology 196: 446-452.
Smirnoff N (1993). The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist 125: 27-58.
Terbea M, Vranceanu AV, Petcu E, Craiciu DS & Micut G (1995). Physiological response of sunflower plants to drought. Romanian Agricultural Research 3: 61-67.
Terzi R, Sağlam A, Kutlu N, Nar H & Kadıoğlu A (2010). Impact of soil drought stress on photochemical efficiency of photosystem II and antioxidant enzyme activities of Phaseolus vulgaris cultivars. Turkish Journal of Botany 34: 1-10.
Türkan İ, Bor M, Özdemir F & Koca H (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought- tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science 168: 223-231.
Wang SY, Jiao H & Faust M (1991). Changes in ascorbate, glutathione and related enzyme activities during thiodiazuron-induced bud break of apple. Physiologia Plantarum 82: 231-236.
Wang W, Vinocur B & Altman A (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1-14.
Zhang J & Kirkham MB (1996). Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytologist 132: 361- 373.