Alleviation of Salinity-Induced Dormancy by Growth Regulators in Wheat Seeds

The effect of 2 growth regulators, betaine and thiourea, was examined to learn the extent to which they can alleviate salinity-induced dormancy of wheat seeds. Germination velocity (as measured by Timson index) progressively decreased with increasing salinity, while thiourea and betaine, alone or in combination, elevated the germination velocity of wheat seeds. Timson index showed significant improvement in germination velocity when thiourea was used at 225 mM NaCl. Likewise, the adverse effect of salinity on growth parameters, like root and shoot lengths and fresh and dry weights, was also alleviated considerably when growth regulators were added. The application of growth regulators in combination with NaCl resulted in increased level of reducing sugars in the embryo and a decrease in the endosperm coupled with enhanced amylase activity, suggesting significant recovery in the mobilization rate of soluble sugars from endosperm to embryo. Thiourea was more effective in the mobilization process compared to betaine. Significance of growth regulators in alleviating the salt stress in crop plants is discussed.

Alleviation of Salinity-Induced Dormancy by Growth Regulators in Wheat Seeds

The effect of 2 growth regulators, betaine and thiourea, was examined to learn the extent to which they can alleviate salinity-induced dormancy of wheat seeds. Germination velocity (as measured by Timson index) progressively decreased with increasing salinity, while thiourea and betaine, alone or in combination, elevated the germination velocity of wheat seeds. Timson index showed significant improvement in germination velocity when thiourea was used at 225 mM NaCl. Likewise, the adverse effect of salinity on growth parameters, like root and shoot lengths and fresh and dry weights, was also alleviated considerably when growth regulators were added. The application of growth regulators in combination with NaCl resulted in increased level of reducing sugars in the embryo and a decrease in the endosperm coupled with enhanced amylase activity, suggesting significant recovery in the mobilization rate of soluble sugars from endosperm to embryo. Thiourea was more effective in the mobilization process compared to betaine. Significance of growth regulators in alleviating the salt stress in crop plants is discussed.

___

  • Aldosaro J, Mantila A & Nicholas G (1981). Effect of ABA, fusicoccin and thiourea on germination, K+and glucose uptake in chick pea seeds of different temperatures. Physiol Plant, 52: 353-362.
  • Ashraf M, Afaf YR, Qureshi MS, Sarwar G & Naqvi MH (2002). Salinity induced changes in α-amylase and protease activities and associated metabolism in cotton varieties during germination and early seedling growth stags. Acta Physiol Plant 24: 37-44.
  • Bano A & Aziz N (2003). Salt and drought stress in wheat and the role of abscisic acid. Pak J Bot 35: 871-883.
  • Bewley JD & Black M (1994). Cellular event during germination and seedling growth. In: Seeds. Physiology of Development and Germination. J. D. Bewley and M. Black (eds). Vol II, pp.147-191. New York. Plenum Press.
  • Bolarin MC, Santa Cruz A, Cayuela E & Perez-Alfocea F (1995). Short- term solute changes in leaves and roots of cultivated and wild tomato seedlings under salinity. J Plant Physiol 147: 463-468.
  • Bray EA (1997). Plant responses to water deficit. In: Trends in Plant Science. Vol 2, pp. 48–54.
  • Dale JE (1988). The control of leaf expansion. Ann Rev Plant Physiol 39: 267-295.
  • Dash M & Panda SK (2001). Salt Stress Induced Changes in Growth and Enzyme Activities in Germinating Phaseolus muingo seeds. Biol Plant 44: 587-589.
  • Decker LA (1977). “Worthington enzymes manual” pp.173-176. Worthington Biochemical Corp. Freehold, NJ.
  • Dubois M, Guillies KA, Hamilton JK, Rebers PA & Smith F (1956). Colorimetric method for determination of sugars and related substances. Ann Chem 28: 350-356.
  • Esashi Y, Ohara Y, Okzaki M & Hishinuma K (1979). Control of cocklebur seed germination by nitrogenous compound like nitrite, hydroxylamine, thiourea, azide and cyanide. Plant and Cell Physiol 20: 349-361.
  • Gill PK & Singh P (1985). Effect of salinity on carbohydrate metabolism during paddy (Oryza sativa) seed germination under salt stress condition. J Exp Biol 23: 384-386.
  • Gill PK, Sharma AD, Singh P & Bhullar SS (2002). Osmotic stress- Induced changes in germination, growth and soluble sugar content of Sorghum bicolor (L.) Moench seeds. Bulg J Plant Physiol 28: 12-25.
  • Gorham J, Hughes LY & Wyn Jones RG (1981). Low-molecular-weight carbohydrates in some salt stressed plants. Physiol Plant 53: 27- 33.
  • Gorham J (1995). Mechanisms of salt tolerance of halophytes. In: Halophytes and biosaline agriculture. R. Chouk–Allah, C.V. Malcolm, A. Hmdy (eds). , Vol. I pp.207-223. New York. Marcel Dekker Inc.
  • Hanson AD & Hitz WD (1982). Metabolic responses of mesophytes to plant water deficits. Ann Rev Plant Physiol 33: 163–203.
  • Ingram J & Bartlets D (1996). The molecular basis of dehydration tolerance in plants. Ann Rev Plant Physiol & Plant Mol Biol 47: 77–403.
  • Ismail AMA (1990). Germination eco-physiology in population of Zygophyllum qatarenses Hadidi from contrasting habitats. J Arid Environ 18: 5-194.
  • Jacobsen JV, Hanson AD & Chandler PC (1986). Water stress enhances expression of an -amylase gene in barley leaves. Plant Physiol 80: 350–359.
  • Kabar KS & Baltepe S (1989). Effect of kinetin and gibberellic acid in overcoming high temperature and salinity (NaCl) stresses on the germination of barley and lettuce seeds. Phyton 30: 30-65.
  • Kameli A & Lösel DM (1995). Contribution of carbohydrates and solutes to osmotic adjustment in wheat leaves under water stress. J Plant Physiol 145: 363-366.
  • Khan MA & Ungar IA (1997). Alleviation of seed dormancy in the desert for Zygophyllum simplex L. from Pakistan. Ann Bot 80: 395-400.
  • Khan MA & Ungar IA (2001). Alleviation of salinity stress and the response to temperature in two seed morphs of Halopyrum mucronatim (Poaceae). Aust J Bot 49: 777-783.
  • Khan MA, Ungar IA & Gul B (1998). Action of osmotica and growth regulators in alleviating the effect of salinity on the germination of dimorphic seeds of Arthrocnemum indicum L. Int J Plant Sci 159: 313-317.
  • Khan MA, Ungar IA & Showalter AM (1999). The effect of salinity on growth ion content and osmotic relation ion Halopyrum mucronatum (L.) Stapf. J plant nutrition 22: 191-204.
  • Leopold AC (1990). Coping with desiccation. In: Stress response in plants: adaptation and acclimation mechanisms. R.G. Alscher and J.R.Cumming (eds). pp. 37–56 New York. Wiley-Liss
  • Macleod AM & Orquodale MC (1958). Water soluble carbohydrates of seeds of the Gramineae. New Phytol 57: 168-182.
  • Mayer AM & Poljakoff-Mayber A (1975). The germination of seeds. 2nd Edition, New York Pergamon Press.
  • Nakamura K, Ohta M, Yoshida N & Nakamura K (1991). Sucrose- induced accumulation of ß-amylase occur concomitant with the accumulation of starch and sporamin in leaf-petiole cuttings of sweet potato. Plant Physiol 96: 902–909.
  • Okusanya OT & Ungar IA (1983). The effects of time of seed production on the germination response of Spergularia marina. Physiol Plant 59: 335-342.
  • Prado FE, Boero C, Gallardo M & Gonzalez JA (2000). Effect of NaCl on germination, growth, and soluble sugar content in Chenopodium quinoa Willd. seeds. Bot Bull Acad Sinica 41: 27- 34.
  • Poljakoff-Mayber A, Somers GF, Werker E & Gallagher JL (1994). Seeds of Kosteletzkya virginica (Malvaveae): Their structure, germination, and salt tolerance. II. Germination and salt tolerance. Am J Bot 81: 54-59.
  • Plyler DB & Proseus TE (1996). A comparison of seed dormancy characteristics of Spartina alterniflora (Poaceae). Am J Bot 83: 11-14.
  • Rozema J, Buizer DAG & Fabritius HE (1978). Population dynamics of Glaux maritima and eco-physiological adaptations to salinity and in inundation. Oikos 30: 539-548.
  • Schobert B (1977). Is there an osmotic regulatory mechanism in algae and higher plants? J Theo Biol 68: 17-26.
  • Sharma A, Sharma S & Gupta MN (2000). Purification of wheat germ amylase by precipitation. Prot Exp &Purification 18: 111-114.
  • SPSS. 1994 SPSS: SPSS 11.0 for windows update. SPSS, Chicago. USA
  • Sultana N, Ikeda T & Kashem MA (2000). Amelioration of NaCl. Stress by gibberellic acid in wheat seedling. Bull Faculty of Agric 52: 71- 76.
  • Tirmizi SAS (1988). Factors affecting germination and dormancy of Honkenys peploides (L.) Ehrh. I. Improvement of germination. Pak J Sci & Ind Res 31: 842-847.
  • Ungar IA (1996). Effect of salinity on seed germination, growth and ion accumulation of Atriplex patula (Chenopodiaceae). Am J Bo 83: 604-607.
  • Ungar IA (1991). Ecophysiology of Vascular Halophytes. CRC press, Boca Raton.
  • Ungar IA (1984). Alleviation of seed dormancy in Spergularia marina. Botanical Gazette 145: 33-36.
  • Wang Z, Quebedeaux B & Stutte GW (1996). Partitioning of (14C) glucose into sorbitol and other carbohydrates in apple under water stress. Aust J Plant Physiol 23: 245-251.
  • Wang Z & Stutte GW (1992). The role of carbohydrates in active osmotic adjustment in apple under water stress. J Am Soc Hort Sci 117: 816-823.
  • Yoshiba Y, Kiyosue T, Nkashima K, Yamaguchi-Shinozaki Y & Shinozaki K (1997). Regulation of levels of proline as an osmolyte in plants under water stress. Plant and Cell Physiol 38: 1095-1102.
  • Yoshiyama M, Mruyama A, Atsumi T & Esashi Y (1996). Mechanism of action of C2H2 in promoting the germination of cocklebur seeds. III. A further enhancement of priming effect with nitrogenous compounds and C2H2 responsiveness of seeds. Aust J Plant Physiol 23: 519-525.