A new subspecies of Saxifraga artvinensis V.A.Matthews (Saxifragaceae) from northeastern Anatolia

A new subspecies of Saxifraga artvinensis V.A.Matthews (Saxifragaceae) from northeastern Anatolia

Saxifraga artvinensis V.A.Matthews, which is endemic to Turkey, was discovered on Tiryal Mountain (A8 Artvin, northeasternTurkey). Approximately 175 km from its type locality, a new population of the species was discovered, in the Picea orientalis forest inthe Altındere Valley National Park, in A7 Trabzon (northeastern Turkey). A comparison with the known population of S. artvinensisconfirmed that this new population represented a new taxon and is described as S. artvinensis subsp. meryemii Terzioğlu & Coşkunç. Anidentification key and comparison with closely related taxa, based on both morphological and molecular properties, are also provided.The studied samples, belonging to subsp. artvinensis and subsp. meryemii, had identical sequences in the entire internal transcribedspacer region. On the other hand, subsp. meryemii was easily distinguishable from subsp. artvinensis by its hairy flowering stem, 3–8flowers, subsessile pedicels, and sepals hairy at base.

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  • Anşin R (1979). Trabzon-Meryemana Araştırma Ormanı Florası ve Saf Ladin Meşcerelerinde Floristik Araştırmalar. Trabzon, Turkey: Karadeniz Gazetecilik ve Matbaacılık A.Ş. (in Turkish).
  • Aslan S (2012). Saxifaraga. In: Güner A, Aslan S, Ekim T, Vural M, Babaç MT (editors). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul, Turkey: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Deneği Yayını, pp. 843-845 (in Turkish).
  • Doyle JJ, Dolye JL (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15.
  • Edgar RC (2004). MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792-1797.
  • Farris JS, Albert VA, Kallersjö M, Lipscomb D, Kluge AG (1996). Parsimony Jackknifing Outperforms Neighbor-Joining. Cladistics 12: 99-124.
  • Fırat M (2016). Saxifraga hakkariensis (Saxifragaceae), a new species from Hakkari province (Turkey) belonging to section Porphyrion. Phytotaxa 289 (2): 181-187.
  • Gornall RJ (1987). An outline of a revised classification of Saxifraga L. Botanical Journal of the Linnean Society 95: 273-292.
  • Gültepe M, Uzuner U, Coşkunçelebi K, Beldüz AO, Terzioğlu S (2010). Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany 34: 147-157.
  • IUCN (2014). Guidelines for Using the IUCN Red List Categories and Criteria, Version 11. Prepared by the Standards and Petitions Subcommittee. Available from: http://www.iucnredlist.org/ documents/RedListGuidelines.pdf. (accessed: 25 Jan 2018).
  • Matthews VA (1972). Saxifraga L. In: Davis PH (editor). Flora of Turkey and the East Aegean Islands. Vol. 4. Edinburgh: Edinburgh University Press, pp. 249-259.
  • Marchese C (2015). Biodiversity hotspots. A shortcut for a more complicated concept. Global Ecology and Conservation 3: 297-309.
  • Müller K (2004). PRAP-Computation of bremer support for large data sets. Molecular Phylogenetics and Evolution 31: 780-782.
  • Müller K (2005). SeqState: Primer design and sequence statistics for phylogenetic DNA datasets. Applied Bioinformatics 4 (1): 65- 69.
  • Müller K, Müller J, Quandt D (2010). PhyDE: Phylogenetic Data Editor, version 0.9971. Available from: http://www.phyde.de/ index.html. (accessed: 10 Jan 2018).
  • Nixon KC (1999). The parsimony watchet, a new method for rapid parsimony analysis. Cladistics, 15: 407-414.
  • Palabaş-Uzun S, Anşin R (2006). Subalpine and Alpine flora of Altındere Valley (Maçka, Trabzon). Turkish Journal of Botany 30: 381-398.
  • Simmons MP, Ochoterena H (2000). Gaps as characters in sequencebased phylogenetic analyses. Systematic Biology 49 (2): 369- 381.
  • Solomon J, Shulkina T, Schatz GE (2013). Red List of the Endemic Plants of the Caucasus: Armenia, Azerbaijan, Georgia, Iran, Russia, and Turkey. Monographs in Systematic Botany from the Missouri Botanical Garden (MSB) 125. Missouri Botanical Garden Press, Saint Louis.
  • Soltis DE (2007). Saxifragaceae. In: Kubitzki K (editor). The Families and Genera of Vascular Plants, Vol: 9. New York, USA: Springer, pp. 418-435.
  • Swofford DL (2002). PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods), Sinauer Associates, Sunderland MA. 144 p.
  • Terzioğlu S, Tüfekçioğlu A, Küçük M (2015). Vegetation and plant diversity of high-altitude mountains in eastern Karadeniz (Black Sea) region of Turkey and climate change interactions, Part 15. In: Öztürk M, Hakeem KR, Faridah-Hanum I, Efe R (editors). Climate Change Impacts on High-Altitude Ecosystems. Switzerland: Springer International Publishing, pp. 383-408.
  • Tkach N, Röser M, Miehe G, Muellner-Riehl AN, Ebersbach J et al. (2015). Molecular phylogenetics, morphology and a revised classification of the complex genus Saxifraga (Saxifragaceae). Taxon 64 (6): 1159-1187.
  • Uzun A, Terzioğlu S (2008). Vascular flora of forest vegetation in Altındere Valley (Maçka-Trabzon). Turkish Journal of Botany 32: 135-153.
  • White TJ, Bruns T, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White TJ (editors). PCR protocols: A Guide to Methods and Application, San Diego: Academic Press. pp. 315-322.