Thiamin-induced variations in oxidative defense processes in white clover (Trifolium repens L.) under water deficit stress
Vitamins are regarded as vital growth substances. They act as cofactors of various key enzymes involved in metabolism and
also have vital influence on metabolic activities of plants. They are believed to play a significant role in plants against abiotic stresses,
particularly during water stress. The current experiment was conducted to evaluate the role of exogenously applied thiamin on two white clover cultivars, Super Late Fsd and Layalpur Late, at varying [100% (control), 80%, and 60% field capacities] water regimes. A
significant suppression was noted in plant biomass, plant height, chlorophyll a and b contents, total soluble proteins, and shoot and root P, Ca2+, and K+ contents particularly at 60% field capacity in both cultivars. Drought stress had no significant effect on phenolic contents,
but MDA concentration was increased in white clover cultivar Layalpur Late at both 80% and 60% drought levels. However, in Super
Late Fsd, it was increased at 80% and decreased at 60% field capacity. Drought stress significantly enhanced levels/activities of ascorbic
acid and antioxidant enzymes (CAT, SOD, and POD). Foliar spray of 50 mM thiamin improved the shoot and root fresh weights
and shoot dry weight. Varying levels of thiamin were found significantly effective for shoot length, root length, and photosynthetic pigments (chlorophyll a and b
). However, no considerable change was observed in ascorbic acid, total soluble proteins, and activities of
antioxidants (SOD, CAT, and POD) due to exogenous spray of thiamin on two white clover cultivars. Of the two white clover cultivars,
Super Late Fsd was better in shoot and root P and Layalpur Late in root dry weight and root length. Overall, 100 mM of thiamin
was found effective in improving growth of both white clover cultivars, which can be attributed to thiamin-induced improvement in
photosynthetic pigments and phenolic contents.
Keywords:
Water deficit stress, Trifolium repens, thiamin, exogenous application, oxidative defense system,
___
- Ahmad P, Ashraf M, Hakeem KR (2014). Potassium starvation- induced oxidative stress and antioxidant defense responses in Brassica juncea . J Plant Interact 9: 1-9.
- Ajjawi I, Tsegaye Y, Shintani D (2007). Determination of the genetic, molecular and biochemical basis of the Arabidopsis thaliana thiamin auxotroph th1. Arch Biochem Biophys 459: 107-114.
- Akram MS, Ashraf M (2012). Alleviation of adverse effects of salt stress on sunflower ( Helianthus annuus L.) by exogenous application of potassium nitrate. J Appl Bot Food Qual 83: 19-27.
- Al-Hakimi ABM, Hamada AM (2011). Ascorbic acid, thiamine or salicylic acid induced changes in some physiological parameters in wheat grown under copper stress. Plant Protect Sci 47: 92- 108.
- Alipoor M, Mohsenzadeh S (2012). Effect of vitamin B complex on some biochemical parameters of Aloe vera L. under nickel and cadmium stress. J Med Plants By-Products 2: 107-115.
- Arnon DI (1949). Copper enzymes in isolated chloroplast polyphenol oxidase in Beta vulgaris . Plant Physiol 24: 1-15.
- Ashraf M, Athar HR, Harris PJC (2008). Some prospective strategies for improving crop salt tolerance. Adv Agron 97: 45-110.
- Ashraf MA, Ashraf M, Ali Q (2010). Response of two genetically diverse wheat cultivars to salt stress at different growth stages: leaf lipid peroxidation and phenolic contents. Pak J Bot 42: 559- 566.
- Atkinson NJ, Urwin PE (2012). Interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63: 3523-3543.
- Cakmak I, Horst WJ (1991). Effects of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean ( Glycine max ). Plant Physiol 83: 463-468.
- Chance M, Maehly AC (1955). Assay of catalases and peroxidases. Meth Enzymol 2: 764-817.
- El-Aziz A, Nahed G, El-Quesni, Fatma EM, Farahat MM (2007) Response of vegetative growth and some chemical constituents of Syngonium podophyllum L. to foliar application of thiamine, ascorbic acid and kinetin at Nubaria. World J Agric Sci 3: 301- 305.
- Emam MM, El-Sweify AH, Helal NM (2011). Efficiencies of some vitamins in improving yield and quality of flax plant. Afr J Agric Res 6: 4362-4369.
- Fiala K, Blanka V, Ladanyi Z (2014). Drought severity and its effect on agricultural production in the Hungarian-Serbian cross- border area. J Environ Geogr 7: 43-51.
- Giannopolitis CN, Ries SK (1977). Superoxide dismutases I. Occurrence in higher plants. Plant Physiol 59: 309-314.
- Goyer A (2010). Thiamine in plants: aspects of its metabolism and functions. Phytochemistry 71: 1615-1624.
- Hajheidari M, Eivazi A, Buchanan BB (2007). Proteomics uncovers a role for redox in drought tolerance in wheat. J Proteome Res 6: 1451-1460.
- Julkenen-Titto R (1985). Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem 33: 213-217.
- Kaur K, Gupta AK, Kaur N (2007). Effect of water deficit on carbohydrate status and enzymes of carbohydrate metabolism in seedlings of wheat cultivars. J Biochem Biophys 44: 223-230.
- Kaya C, Ashraf M, Sonmez O, Tuna AL, Polat T, Aydemir S (2015) Exogenous application of thiamin promotes growth and antioxidative defense system at initial phases of development in salt-stressed plants of two maize cultivars differing in salinity tolerance. Acta Physiol Plant 37: 1741.
- Kruger NJ, von Schaewen A (2003) The oxidative pentose phosphate pathway: structure and organization. Curr Opin Plant Biol 6: 236-246.
- Li Z, Peng Y, Ma X (2013). Different response on drought tolerance and post-drought recovery between the small-leafed and the large-leafed white clover ( Trifolium repens L.) associated with anti-oxidative enzyme protection and lignin metabolism. Acta Physiol Plant 35: 214-222.
- Losak T, Hlusek J, Filipcik R (2010). Effect of nitrogen fertilization on metabolism of essential and non-essential amino acids yield- grown grain maize ( Zea mays L.). Plant Soil Environ 56: 574- 579.
- Mateikene I, Bandzhyulene R, Ozheraitene M, Bluzmanas P (1988). Uptake and distribution of 14 C-thiamin in barley caryopses and plants. Soviet Plant Physiol 35: 881-889.
- Mitter H, Schmid E, Schneider UA (2015). Modelling impacts of drought and adaptation scenarios on crop production in Austria. Erschienen im Jahrbuch der Österreichischen Gesellschaft für Agrarökonomie 24: 223-232.
- Mozafar A, Oertli JJ (1992) Uptake and transport of thiamin (vitamin-B1) by barley and soybean. J Plant Physiol 139: 436- 442.
- Mukherjee SP, Choudhuri MA (1983). Implication of water stress- induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Plant Physiol 58: 166- 70.
- Nahed GAA, Lobna ST, Soad MMI (2009). Some studies on the effect of putrescine, ascorbic acid and thiamine on growth, flowering and some chemical constituents of gladiolus plants at Nubaria. Ozean J Appl Sci 2: 22-29.
- Pryor HN, Lowther WL (2002). Symbiotic relationship between Rhizobium leguminosarum biovar trifolii and Trifolium nigrescens . New Zeal J Agric Res 45: 145-149.
- Ranjbar B, Sharafzadeh S, Alizadeh O (2014). Growth and essential oil responses of German chamomile to thiamine and ascorbic acid. Bull Environ Pharmacol Life Sci 3: 51-53.
- Rapala-Kozik M, Wolak N, Kujda M (2012). The up-regulation of thiamin (vitamin B-1) biosynthesis in Arabidopsis thaliana seedling under salt and osmotic stress condition is mediated by abscisic acid at the early stage of this stress response. BMC Plant Biol 12: 2.
- Sarvajeet SG, Tuteja N (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48: 909-930.
- Sayed SA, Gadallah MAA (2002). Effects of shoot and root application of thiamin on salt-stressed sunflower plants. Plant Growth Regul 36: 70-81.
- Shafiq S, Akram NA, Ashraf M (2015). Does exogenously applied trehalose alter oxidative defense system in the edible part of radish ( Raphnus sativus L.) under water deficit conditions? Sci Hort 185: 68-75.
- Strauss F, Moltchanova E, Schmid E (2013). Spatially explicit modeling of long-term drought impacts on crop production in Austria. Am J Climate Change 2: 1-11.
- Sun J, Gu J, Zeng J (2013). Changes in leaf morphology, antioxidant activity and photosynthesis capacity in two different drought- tolerant cultivars of chrysanthemum during and after water stress. Sci Hort 161: 249-258.
- Tunc-Ozdemir M (2009). Thiamin confers enhanced tolerance to oxidative stress in Arabidopsis. Plant Physiol 151: 421-432.
- Turkan I (2011). Plant Responses to Drought and Salinity Stress: Developments in a Post-Genomic Era (Vol. 57). San Diego, CA, USA: Academic Press.
- Wang S, Liu P, Chen D (2015). Silicon enhanced salt tolerance by improving the root water uptake and decreasing the ion toxicity in cucumber. Front Plant Sci 6: 759-768.
- Wang X, Cai J, Jiang D (2011). Pre-anthesis high-temperature acclimation alleviates damage to the flag leaf caused by post- anthesis heat stress in wheat. J Plant Physiol 168: 585-593.
- Waseem M, Ajmal M, Lee JH (2016). Multivariate drought assessment considering the antecedent drought conditions. Water Res Manag 1: 1-11.
- Yousef AA, Talaat MI (2003). Physiological response of rosemary plants to some vitamins. Egypt Pharmaceut J 1: 81-93
- ISSN: 1300-008X
- Yayın Aralığı: Yılda 6 Sayı
- Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler
Katerina RUSEVSKA, Franciso De Diego CALONGE, Mitko KARADELEV, María Paz MARTÍN
Abuzer ÇELEKLİ, Assane Anabi TOUDJANI, Emine Yonca GÜMÜŞ, Seda KAYHAN, Hacı Ömer LEKESİZ, Tolga ÇETIN
Karoline Aparecida Felix RIBEIRO, José ángel SÁNCHEZ AGUDO, Silvia SÁNCHEZ DURÁN, Ester VEGA ELICES, José SÁNCHEZ SÁNCHEZ
Süleyman Mesut PINAR, Hüseyin EROĞLU
Mamdouh NEMAT ALLA, Enas BADRAN, Fozia MOHAMMED
Muhammad Adeel GHAFAR, Nudrat Aisha AKRAM, Muhammad ASHRAF, Muhammad Yasin ASHRAF, Muhammad SADIQ
Qudsia YOUSAFI, Hafsa Ahmad ALI, Hamid RASHID, Muhammad Saad KHAN