Vakum ile paketleniş sosis örneklerinden pediosin üreten Pediococcus pentosaceus Pep 1 suşunun izolasyonu ve karekterizasyonu

Pediococcus pentosaceus Pep1 was isolated from vacuum-packed sausages, and it was shown to produce a potentially novel antimicrobial agent active against several species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus and Listeria, many of which are associated with food spoilage and health hazards of food origin. Gram-negative organisms such as Yersinia enterocolitica and Escherichia coli were not inhibited by this antimicrobial agent. Since the antimicrobial agent was found to be sensitive to proteolytic enzymes, resistant to heat and organic solvents and active over a wide range of pH values, between 3 and 8, apparently most stable in the lower part of that range, it was identified as a bacteriocin and was designated as pediocin P.

Isolation and characterization of pediocin producing Pediococcus pentosaceus Pep 1 from vacuum-packed sausages

Pediococcus pentosaceus Pep1 was isolated from vacuum-packed sausages, and it was shown to produce a potentially novel antimicrobial agent active against several species of Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Staphylococcus, Enterococcus, Bacillus and Listeria, many of which are associated with food spoilage and health hazards of food origin. Gram-negative organisms such as Yersinia enterocolitica and Escherichia coli were not inhibited by this antimicrobial agent. Since the antimicrobial agent was found to be sensitive to proteolytic enzymes, resistant to heat and organic solvents and active over a wide range of pH values, between 3 and 8, apparently most stable in the lower part of that range, it was identified as a bacteriocin and was designated as pediocin P.

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  • 1. Diebel, R.H., Wilson, G.D., Niven, C.F., Microbiology of meat curing. IV. A lyophilized Pediococcus cerevisiae starter culture for fermented sausage. Appl Microbiol 9: 239-243, 1961.
  • 2. Smith, J.L., Palumbo, S. A., Use of starter cultures in meats. J Food Prot 46: 997-1006, 1983.
  • 3. Tagg, J.R., Dajani, A.S., Wannamaker, L.W., Bacteriocins of gram-positive bacteria. Bacteriol Rev 40: 722-756, 1976.
  • 4. Pederson, C.S., The genus Pediococcus. Bacteriol Rev 13: 225-232, 1949.
  • 5. Franklin, J.G., Sharpe, M.E., The incidence of bacteria in cheese milk and Cheddar cheese and their association with flavor. J Dairy Research 30: 87-99, 1963.
  • 6. Bhunia, A.K, Johnson, M.C., Ray, B., Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol 65: 261-268, 1988.
  • 7. Gonzalez, C.F., Kunka, B.S., Plasmid associated bacteriocin production and sucrose fermentation in Pediococcus acidilactici. Appl Environ Microbiol 53: 2534-2538, 1987.
  • 8. Hoover, D.G., Walsh, KP.M., Kolaetis, M., Daly, M.M., A bacteriocin produced by Pediococcus species associated with a 5.5-megadalton plasmid. J Food Protect 51: 29-31, 1988.
  • 9. Osmanağaoğlu, Ö., Gündüz, U., Beyatlı, Y., Çökmüş, C., Purification and characterization of pediocin F, a bacteriocin produced by Pediococcus acidilactici F. Tr J of Biology, 22: 217-228, 1998.
  • 10. Daeschel, M.A., Klaenhammer, T.R., Association of 13.6-megadalton plasmid in Pediococcus pentosaceus with bacteriocin activity. Appl Environ Microbiol 50: 1538-1541, 1985.
  • 11. Graham, D.C., McKay, L.L., Plasmid DNA in strains of Pediococcus cerevisiae and Pediococcus pentosaceus. Appl Environ Microbiol 50: 532-534, 1985.
  • 12. Henderson, J.T., Chopko, A.L., van Wassenaar, P.D., Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC1.0. Arch Biochem Biophys 295: 5-12, 1992.
  • 13. Motlagh, A.M., Bhunia, A.K., Szostek, F., Hansen, T.R., Johnson, M.C., Ray, B.,. Nucleotide and amino acid sequence of pap-gene, pediocin AcH production in Pediococcus acidilactici H. Lett Appl Microbiol 15: 45-48, 1992.
  • 14. Pucci, M.J., Vedamuthu, E.R., Kunka, B.S., Vanderbergh, P.A., Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC1.0. Appl Environ Microbiol 54: 2348-2353, 1988.
  • 15. Costilow, R.N., Coughlin, F.M., Robach, D.L., Ragheb, H.S., A study of the acid-forming bacteria from cucumber fermentations in Michigan. Food Res 21: 27-33, 1956.
  • 16. Etchells, J.L., Costilow, R.N., Anderson, T.E., Bell, T.A., Pure culture fermentation of brined cucumbers. Appl Microbiol 12: 523-535, 1964.
  • 17. Fleming, H.P., Etchells, J.L., Costilow, R.N., Microbial inhibition by an isolate of Pediococcus from cucumber brines. Appl Microbiol 30: 1040-1042, 1975.
  • 18. Rueckert, P.W., Studies on a bacteriocin-like activity produced by Pediococcus pentosaceus effective against grampositive organism. M.S. thesis. Michigan State University, East Lansing, 1979.
  • 19. Spelhaug, S.R., Harlender, S.K., Inhibition of foodborne bacterial pathogens by bacteriocins from Lactococcus lactis and Pediococcus pentosaceus. J Food Prot 52: 856-862, 1989.
  • 20. Piva, A., Headon, D.R., Pediocin A, a bacteriocin produced by Pediococcus pentosaceus FBB61. Microbiology 140: 697-702, 1994.
  • 21. Biswas, S.R., Ray, P., Johnson, M.C., Ray, B., Influence of growth conditions on the production of a bacteriocin, pediocin AcH, by Pediococcus acidilactici H. Appl Environ Microbiol 57: 1265-1267, 1991.
  • 22. Yang, R., Ray, B., Prevalence and biological control of bacteriocin-producing psychrotrophic Leuconostocs associated with spoilage of vacuum-packaged processed meats. J Food Protect. 57: 209-217, 1994.
  • 23. Garvie, E.I., Genus Pediococcus Claussen 1903. p. 1075-1079. In Bergey's Manual of Systematic Bacteriology. Vol. 2, P.H.A. Sneath (ed.), Williams and Wilkins, Baltimore, 1986.
  • 24. Yang, R., Johnson, M.C., Ray, B., Novel method to extract large amounts of bacteriocins from lactic acid bacteria. Appl Environ Microbiol 58: 3355-3359, 1992.
  • 25. Barefoot, S.F., Klaenhammer, T.R., Detection and activity of lactocin B, a bacteriocin produced by Lactobacillus acidophilus. App Environ Microbiol 45: 1808-1815, 1983.
  • 26. Upreti, G.C., Hinsdill, R.D., Isolation and characterization of a bacteriocin from a homofermentative Lactobacillus. Antimicrob Agents Chemother 4: 487-494, 1973.
  • 27. Hurst, A., Nisin. Adv Appl Microbiol 27: 85-123, 1981.
  • 28. Ray, B.: Characteristics and applications of pediocin(s) of Pediococcus acidilactici: Pediocin AcH/PA-1: an overview. Pp 155-197. In Lactic Acid Bacteria: current advances in metabolism, genetics and applications. T. Faruk Bozoğlu and Bibek Ray (ed), NATO ASI Series, Springer Press, Germany, 1996.
Turkish Journal of Biology-Cover
  • ISSN: 1300-0152
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK