Monday Isaiah AKPANABIATU, Friday Effiong UBOH, Theresa Bassey EKANEM, Imeh Ben UMOH, Eyong Ubana EYONG, Sylvanus O. UKAFIA

The effect of interaction of Rauwolfia vomitoria root bark extract with vitamin E on rats' liver enzymes

Daha önceki çalışmamızda, normal Wistar sıçanlarının kalp enzimleri üzerine Rauwolfia vomitoria’nın kök kabuğu özütünün etkisini ve bu kök kabuğu etkinliğini, marker enzimler tarafından belirlendiği gibi, kan basıncı düşürücü ajan olarak, klinik uygulamada bu bitkinin toksisitesi için faydalı bir dizin olabileceğini gösterdik. Bu çalışmada, Rauwolfia vomitoria kök kabuk özütünün etkisi ve E vitamini ile olan etkileşiminin alkalen fosfataz (ALP), alanin amino transferaz (ALT), aspartat amino transferaz (AST), gama glutamat transferaz (γ-GT) gibi karaciğer fonksiyon enzimleri üzerine etkisinin sonuçları ve normal hayvanların karaciğer dokularının histolojik yapısı sunulmuştur. Sonuçlar özütün AST aktivitesinde önemli bir artışa (P ≤ 0,01) sebep olduğunu göstermiştir. Bulgularda etkileşimin γ-GT, ALT ve ALP’nin durumundaki gibi normal veya normalden daha az enzimlerin aktivitesini azalttığını ve Rauwolfia vomitoria’ nın kök kabuk özütünün toksikliği üzerine E vitamininin iyileştirici etkileri olduğunu gösterdi. Histolojik sonuçlarda bu bitkinin muhtemel toksikliği üzerine E vitamininin iyileştirici etkisini doğruladı.

Sıçanların karaciğer enzimleri üzerine E vitamini ile Rauwolfia vomitoria kök kabuk özütünün etkileşiminin etkisi

In our previous paper we demonstrated the influence of Rauwolfia vomitoria root bark extract on cardiac enzymes of normal Wistar rats and the effectiveness of this root bark as a source of antihypertensive agent, as determined by the marker enzymes, would be a useful index for the toxicity of this plant in clinical application. In this paper we present the results of the effect of Rauwolfia vomitoria root bark extract and its interaction with vitamin E on the liver function enzymes, such as alkaline phosphatase (ALP), alanine amino transferase (ALT), aspartate amino transferase (AST), gamma glutamyl transferase (γ-GT), and the histological architectures of the liver tissues of normal animals. The results showed significant (P ≤ 0.01) increase in the activity of AST by the extract. The findings also showed that interaction reduced the activity of enzymes to about normal or less than normal as in the case of γ-GT, ALT, and ALP, indicating the ameliorating effects of vitamin E on the toxicity of Rauwolfia vomitoria root bark extract. Histological results also confirmed the ameliorating effect of vitamin E on possible toxicity of this herb. As vitamin E and Rauwolfia vomitoria root bark extract already have known advantages in the management of cardiovascular disease, concomitant administration of Rauwolfia vomitoria root bark extract with vitamin E may be a better option.

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1. Marcus R, Coulstom AM. Fat soluble vitamin A, K, and E. In: Hardman G, Limbird, LE. eds. Therapeutic (9th ed.). Perganon press, INC, Elmsford, New York; 1996; pp: 1573-1590.
2. Traber MG. Does vitamin E decrease heart attack risk? Summary and implications with respect to dietary recommendations. J Nutr 131(2): 395S-397S, 2001.
3. Mohsun M. Vitamin E. Lancet 345: 170-175, 1995.
4. Traber MG. Utilization of vitamin E. Biofactors 10(2-3): 115- 120, 1999
5. Akpanabiatu MI, Umoh IB, Eyong EU et al. Influence of Rauwolfia vomitoria root bark extract on cardiac enzymes of normal Wistar albino rats. Biopharmaceut 14(13): 273-278, 2006a.
6. Akpanabiatu MI. Effects of the biochemical interactions of vitamins A and E on the toxicity of root bark extract of Rauwolfia vomitoria (Apocynaceae) in Wistar albino rats, PhD, University of Calabar, Calabar, Nigeria, 2006.
7. Gubar SI, Konstantinova EP, Kunakh VA. Medicinal and aromatic plants conference proceeding part (4 of 4)1: 330-337. Maastricht, Netherlands, 1993.
8. Udosen EO, Ojong AS. Hepatotoxic activity of Sacoglottis gabonensis. Pharmaceut Biol 36(5): 368-371, 1998.
9. Akpanabiatu MI, Umoh, IB, Eyong EU et al. Influence of Nauclea latifolia leaf extracts on some hepatic enzymes of rats fed on coconut oil and non-coconut oil meals. Pharmaceut Biol 43(2): 153-157, 2005.
10. Akpanabiatu MI, Umoh, IB, Uboh FE et al. Effect of Nauclea latifolia (Sm. Rubisceae) leaf extracts on some rats serum enzymes of clinical significance. Biopharmaceut 14(13): 297- 304, 2006b.
11. Ugochukwu NH, Babady NE, Cobourne M et al. Effect of Gongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J Biosciences 28(1): 1-5, 2003.
12. Akpanabiatu MI, Igiri AO, Eyong EO et al. Biochemical and histological effects of Eleophorbia drupifera leaf extract in Wistar albino rats. Pharmaceut Biol 41(2): 96- 99, 2003.
13. Nada SA, Bashandy SAE, Negm SA. Evaluation of the hypoglycemic activity of traditional herbal preparation in male diabetic rats. Fitoterapy 68: 240-244, 1997.
14. Abdel-Baset Halim, Omar El-Ahmady, Samar Hassab Allah et al. Biochemical effect of antioxidants on lipids and liver function in experimentally induced liver damage. Ann Clin Biochem 34: 656-663, 1997.
15. Owu DU, Osim EE, Ebong PE. Serum liver enzymes profile of Wistar rats following chronic consumption of fresh or oxidized palm oil diets. Acta Tropica 69: 65-73, 1998
16. Cabrera-Abreu JC, Green A. γ-glutamyltransferase: value of its measurement in paediatrics. Ann Clin Biochem 39: 22-25, 2002.
17. Moss D, Henderson R. Clinical enzymology. In: Burtis CA, Ashwood ER. eds. Tietz Testbook of Clinical Chemistry. WE Saunders, Philadelphia; 1999: pp. 686-689.
18. Vivekananthan DP, Penn MS, Shapp SK et al. Use of antioxidant vitamin E for the prevention of the cardiac disease: Meta analysis of randomized trials. Lancet 36: 2017-2023, 2003.