Zohreh JALALPOUR, Leila SHABANI, Ladan AFGHANI, Majid SHARIFI-TEHRANI, Sayed-Asadollah AMINI

Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that activities of PAL and PPO enzymes and total antioxidants significantly increased in Taxus cells in response to MeJA and S. Maximal activities of lipoxygenase were obtained 48 h after treatment with MeJA (100 µM), S (0.1 µM), and the combination of the 2 elicitors. Results showed that MeJA and S are effective elicitors for increasing phenolic production in Taxus cell suspension cultures, likely through increasing LOX activity followed by an increase in endogenous jasmonate.

Anahtar Kelimeler:

Key words: Antioxidant, lipoxygenase, phenylalanine ammonia-lyase, squalestatin, Taxus

Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Keywords:

Key words: Antioxidant, lipoxygenase, phenylalanine ammonia-lyase, squalestatin, Taxus,

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Axelrod B, Cheesbrough TM, Laakso S (1981). Lipoxygenase from soybeans: EC 1.13. 11.12 Linoleate: oxygen oxidoreductase. Method Enzymol 71: 441–451.
Blechert S, Brodschelm W, Holder S, Kammerer L, Kutchan TM, Mueller MJ, Xia ZQ, Zenk MH (1995). The octadecanoic pathway: signal molecules for the regulation of secondary pathways. P Natl Acad Sci USA 92: 4099–4105.
Bonfill M, Expósito O, Moyano E, Cusidó RM, Palazón J, Piñol MT (2006). Manipulation by culture mixing and elicitation of paclitaxel and baccatin III production in Taxus baccata suspension cultures. In Vitro Cell Dev-Pl 42: 422–426.
Bulgakov VP, Tchernoded G, Veselova M, Fedoreyev S, Muzarok T, Zhuravlev Y (2011). Catechin production in cultured cells of Taxus cuspidata and Taxus baccata. Biotechnol Lett 33: 1879– 1883.
Conceição LFR, Ferreres F, Tavares RM, Dias ACP (2006). Induction of phenolic compounds in Hypericum perforatum L. cells by Colletotrichum gloeosporioides elicitation. Phytochemistry 67: 149–155.
Creelman RA, Mullet JE (1997). Biosynthesis and action of jasmonates in plants. Annu Rev Plant Phys 48: 355–381.
Creelman RA, Tierney ML, Mullet JE (1992). Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. P Natl Acad Sci USA 89: 4938–4941.
Doares SH, Syrovets T, Weiler EW, Ryan CA (1995). Oligogalacturonides and chitosan activate plant defensive genes through the octadecanoid pathway. P Natl Acad Sci USA 92: 4095–4098.
Esen A (1993). β-Glucosidases. In: Esen A, editor. β-Glucosidases: Biochemistry and Molecular Biology. Washington, DC: American Chemical Society, pp. 1–14.
Ganesan P, Kumar CS, Bhaskar N (2008). Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technol 99: 2717– 2723.
Grimes HD, Koetje DS, Franceschi VR (1992). Expression, activity, and cellular accumulation of methyl jasmonate-responsive lipoxygenase in soybean seedlings. Plant Physiol 100: 433–443.
Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992). Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. P Natl Acad Sci USA 89: 2389–2393.
Kajani AA, Mofid MR, Abolfazli K, Tafreshi SAH (2010). Encapsulated activated charcoal as a potent agent for improving taxane synthesis and recovery from cultures. Biotechnol Appl Biotech 56: 71–76.
Keinänen M, Oldham NJ, Baldwin IT (2001). Rapid HPLC screening of jasmonate-induced increases in tobacco alkaloids, phenolics, and diterpene glycosides in Nicotiana attenuata. J Agr Food Chem 49: 3553–3558.
Khalili M, Hasanloo T, Kazemi Tabar SK, Rahnama H (2009). Influence of exogenous salicylic acid on flavonolignans and lipoxygenase activity in the hairy root cultures of Silybum marianum. Cell Biol Int 33: 988–994.
Kim HJF, Fonseca JM, Choi JH, Kubota C (2007). Effect of methyl jasmonate on phenolic compounds and carotenoids of romaine lettuce (Lactuca sativa L.). Plant Biochem 55: 10366–10372.
Koç E, Üstün AS (2012). Influence of Phytophthora capsici L. inoculation on disease severity, necrosis length, peroxidase and catalase activity, and phenolic content of resistant and susceptible pepper (Capsicum annuum L.) plants. Turk J Biol 36: 357–371.
Li HB, Wong CC, Cheng KW, Chen F (2008). Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Sci Technol 41: 385–390.
Li M, Feng S (2009). Study on the effect of methyl jasmonate on total phenolic acids and salvianolic acid B contents in the leaves and stems of Salvia miltiorrhiza. Lishizhen Medicine and Materia Medica Research 2008–2009: 1–10.
Melan MA, Dong X, Endara ME, Davis KR, Ausubel FM, Peterman TK (1993). An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant Physiol 101: 441–450.
Menke FLH, Parchmann S, Mueller MJ, Kijne JW, Memelink J (1999). Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor-induced expression of terpenoid indole alkaloid biosynthetic genes in Catharanthus roseus. Plant Physiol 119: 1289.
Mittler R (2002). Oxidative stress, antioxidants, and stress tolerance. Trends Plant Sci 7: 405–410.
Moon WJ, Yoo BS, Kim DI, Byun SY (1998). Elicitation kinetics of taxane production in suspension cultures of Taxus baccata pendula. Biotechnol Tech 12: 79–81.
Nojiri H, Sugimori M, Yamane H, Nishimura Y, Yamada A, Shibuya N, Kodama O, Murofushi N, Omori T (1996). Involvement of jasmonic acid in elicitor-induced phytoalexin production in suspension-cultured rice cells. Plant Physiol 110: 387–392.
Procopiou PA, Bailey EJ, Bamford MJ, Craven AP, Dymock BW, Houston JG, Hutson JL, Kirk BE, Mccarthy AD (1994). The squalestatins: novel inhibitors of squalene synthase. Enzyme inhibitory activities and in vivo evaluation of C1-modified analogs. J Med Chem 37: 3274–3281.
Rusterucci C, Montillet JL, Agnel JP, Battesti C, Alonso B, Knoll A, Bessoule JJ, Etienne P, Suty L, Blein JP et al. (1999).
Involvement of lipoxygenase dependent production of fatty acid hydroperoxides in the development of the hypersensitive cell death induced by cryptogein on tobacco leaves. J Biol Chem 274: 36446–36455.
Shouwei L, Fengzhi W, Yanling M (2009). Effects of Fusarium wilt pathogen on the enzyme activity of cucumber cultivars of different resistance. Plant Protection 35: 82–85.
Singh G, Gavrieli J, Oakey JS, Curtis WR (1998). Interaction of methyl jasmonate, wounding and fungal elicitation during sesquiterpene induction in Hyoscyamus muticus in root cultures. Plant Cell Rep 17: 391–395.
Singleton VL, Rossi JA (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16: 144–158.
Soliva RC, Elez P, Sebastian M, Martín O (2001). Evaluation of browning effect on avocado purée preserved by combined methods. Innov Food Sci Emerg 1: 261–268.
Vick BA, Zimmerman DC (1984). Biosynthesis of jasmonic acid by several plant species. Plant Physiol 75: 458–461.
Wahid A, Ghazanfar A (2006). Possible involvement of some secondary metabolites in salt tolerance of sugarcane. J Plant Physiol 163: 723–730.
Walters D, Cowley T, Mitchell, A (2002). Methyl jasmonate alters polyamine metabolism and induces systemic protection against powdery mildew infection in barley seedlings. J Exp Bot 53: 747–756.
Wang YF, Yu SH, Dong M, Zhang ML, Huo CH, Shi QW (2010). Chemical studies on Taxus cuspidata. Chem Biodivers 7: 1698– 1716.
Yuan YJ, Li C, Hu ZD, Wu JC, Zeng AP (2002). Fungal elicitorinduced cell apoptosis in suspension cultures of Taxus chinensis var. mairei for taxol production. Process Biochem 38: 193–198.
Yukimune Y, Tabata H, Higashi Y, Hara Y (1996). Methyl jasmonateinduced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nat Biotechnol 14: 1129–1132.
Zheng W, Wang SY (2001). Antioxidant activity and phenolic compounds in selected herbs. J Agr Food Chem 49: 5165–5170.
Zhishen J, Mengcheng T, Jianming W (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64: 555–559.
Zucker M (1965). Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiol 40: 779.