Sigara içen radyoloji teknisyenlerinde trimethoprimin kromozomal düzensizlikler üzerine etkileri

Ayrı ayrı uygulandıklarında kromozomlar üzerinde klaskojenik etki gösterdikleri bilinen trimethoprim, sigara kullanımı ve radyasyon etkenleri birlikte kullanılmıstır. Çalısmamızda sigara tiryakisi 5 saglıklı erkek radyoloji teknisyeninden alınan kan örneklerine invitro olarak 6, 24 ve 48 saatlik süreler için 8, 35 ve 60 mg/ml dozunda trimethoprim uygulanmıstır. Kontrol olarak ayrılan kültürlere ise aynı süreler için kültür çözeltisinden eklenmistir. Her bir bireyden, her bir doz-süre kombinasyonu için 50 adet metafaz “Kromozomal Düzensizlik Içeren Hücre” ve “Yapısal Kromozom Düzensizlik” oranları açısından degerlendirmeye alınmıstır. Düzensizlik Içeren Hücre oranı; kontrol grubunda % 4.8 iken, 8 mg/ml’de % 11.60, 35 mg/ml’de % 14 ve 60 mg/ml’de ise % 17.86'ya yükseldigi belirlenmistir. Yapısal Kromozom Düzensizlikleri oranı; kontrol grubunda % 4.13 iken, 8 mg/ml’de % 11.73 mg/ml’de % 15.33 ve 60 mg/ml’de ise % 19.06'ya yükselmistir. Bulgular üzerinde yapılan istatiksel degerlendirmelerde artan trimethoprim dozuna paralel olarak kromozomal düzensizlik oranının arttıgı (p0.05) belirlenmistir.

The effects of trimethoprim ion the chromosomal aberrations on the radiological techniciances who smoke

Trimethoprim, cigerette smoking and radition is known to have a clastogenic effects on chromosomes when applied to oneby one. In this study all of this aggents have been applied together. 8, 35 and 60 mg/ml doses of trimethoprim applied to the bloods which were taken from five healthy radiology technicians who are cigarette smokers, for the periods of 6, 24 and 48 hours. The same quantity of culture medium was added to the cultures which were separated for control in the same period. For each dose-aplication ;eriod combination, from each individual 50 metaphases were evaluated in relation to the ratio of “The Cells Including Chromosomal Aberrations” and “Structural Chromosome Abberrations”. The ratio of cells including chromosomal aberration was 4.8 % for the control group, it was 11.6 % at 8 mg/ml, 14 % at the 35 mg/ml and 17.86 % at the 60 mg/ml. The ratio structural chromosome aberrations was 4.13 % for the control group, it was 11.73 at the 8 mg/ml, 15.33 % at the 35 mg/ml and 19.06 % at the 60 mg/ml. Statistical evaluat on of findigs showed that the ratio of choromosomal aberrations incereased parellel to the increased dose of tiremthoprim (p0.05).

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