Serological properties of pseudomonas syringae pv. phseolcola isolates collected from Eskişehir

Eskisehir’deki fasulye ekim alanlarında toplanan 36 fasulye tohumunda Pseudomonas syringae pv. phaseolicola bakterisi aranmıs ve izole edilmeye çalısılmıstır. P. s. pv. phaseolicola NCPPB 52 izolatı için spesifik poliklonal antiserum tavsanda üretilmis ve identifikasyon için hem biyokimyasal hemde serolojik testler kullanılmıstır. Pseudomonas syringae pv. phaseolicola olarak teshis edilen 92 izolat ile bu bakterinin standart suslarının ısıya dayanıklı O antijenleri Ouchterlony immunodiffüzyon testi ile karsılastırılmıs ve bütün izolatların ortak antijenik yapıya sahip oldukları görülmüstür.

Eskişehir'den toplanan Pseudomonas syringae pv.phseolicola izolatlarının serolojik özellikleri

Thirty six bean seed samples collected from bean fields in Eskisehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure.

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