Paraquatın farelerin (Mus musculus-Swiss albino) farklı dokularında bazı enzimlerin aktiviteleri üzerine etkileri

The effects of the LD30 (20 mg.kg-1) dose of paraquat, a herbicide, were investigated on creatine kinase (CK), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT) activities in the liver, kidney and lung of mice after 2, 4, 8, 16, 32, 64 and 72 hours. After paraquat was given intraperitoneally to Mus musculus Swiss albino mice, the control groups were injected with physiological saline. The results were evaluated using the autoanalyzer as U/L. Even though significant increases in the liver tissue LDH and GGT, kidney tissue CK and lung tissue CK and LDH activities were noted when compared to those of the control group (P0.05). Although there were significant increases observed in GPT enzyme activities in the liver and kidney tissue at the beginning of the experiment, these activities were found to be at nearly the same level as those of the control group. Liver tissue GOT activity was lower in comparison with the control group at first but it was found to be statistically insignificant at 8 hours and during the subsequent experiment time periods. A decrease was observed in the CK activity in the liver but a significant increase and then a decrease was observed in the LDH activity in the liver. Consequently, it was found that paraquat caused some changes (increases then decreases) in CK, GOT, GPT, LDH and GGT enzyme activity in different tissues of mice. The causes of the increases and decreases in enzyme activities are discussed.

The effect of paraquat on the activity of some enzymes in different tissues of mice (Mus musculus- Swiss albino)

PDF

___

1. DeGray J.A., Ramakrishna Rao D.N., Mason R.P. Reduction of Paraquat and Related Bipyridylium Compounds to Free Radical Metabolites by Rat Hepatocytes. Archives of Biochem. and Biophysics. 289, No.1, August 15, p. 145- 152, 1991.
2. Asal S. Bazı Pestisitlerin Mutajenik etkileri Üzerine Arafltırmalar. Doğa Bilim Derg. D-2, 9 : 1,72 - 78, 1985.
3. Öztürk S. Tarım ilaçları. 1990 Hasad yayıncılık ve Renk Ofset Istanbul
4. Newsholme E.A. and Leech A.R. Biochemistry for the Medical Sciences. p.158, John Wiley and Sons Ltd. Great Britain, 1983.
5. Bagetta G., Corasanita M.T., Lannone M., Mistoco G., Stephenson J.D. Production of Limbic Motor Seizures and Brain Damage by Systemic and Intracerebral Injections of Paraquat in Rats. Pharm. - Toxic. 71, p. 443-448,1992.
6. Kojima S., Miyazaki Y., Honda T., Kiyozumi M., Shimada H. and Funakoshr T. Effect of L cystine on toxicity of paraquat in mice. Toxicology Letters. 6075-82 1992.
7. Zar Jerrold, H. Biostatistical Analysis 1984 Prentice-Hall International (UK) limited, London, p 206-234.
8. Amena K., Fuke C., Shirakawa Y., Ogura S., Ameno S., Kiriu T., Kinoshita H., Ijiri I. Different distribution of paraquat and diquat in human poisoning cases after ingestion of a combined herbicide. Arch. Toxicol 68: 134-137 1994.
9. Palmeira C.M., Moreno A.J., Madeira V.M.C., Metabolic alterations in hepatocytes promoted by the herbicides paraquat, dinoseb and 2,4-D. Arch Toxicol. 68: p. 24-31, 1994.
10. Saenz M.E., Acorinti J., Tortorelli M. Toxicity of paraquat to a green alga Scenedesmus acutus. J. Environ Sci. Health, B 2812, P. 193-204 1993.
11. Tamaru N., Watanabe K., Yoshida M., PGI2 Production and Angiotensin Converting enzyme Activity in Cultured Porcine Pulmonary Artery Endothelial Cells Treated with Paraquat. Prostaglandins Leukotrienes and Essential Fatty Acids. 48, p. 291-296, 1993.
12. Puapairoj P., Cui L., Ogawa K., Akagi K., Hasegawa R., Iro N. Effect of ethanol on paraquat toxicity in F344 rats. Fd Chem. Toxic. 32, No 4, p 379-386 1994.
13. WHO (World Health Organization), Environmental Health Criteria 39, Paraquat and Diquat. Geneva. 1984.
14. Noguchi N., Yamamoto H., Misawa S. Effect of Paraquat on Mg+2-ATPase activity in rat liver. Arch. Environ. Contam. Toxicol. 24, 483-486 1993.
15. Nagao M., Takatori T., Inoue K., Shimizu M., Terazawa K., Akabane H. Immuno- histochemical localization and dynamics of paraquat in small intestine, liver and kidney. Toxicology. 63, p. 167-182, 1990.
16. Nagao M., Zhang W., Itakura Y., Kobayashi M., Yamada V., Yagi K., Oono T., Takatari T., Immunohistochemical localization and dynamics of paraquat in the stomach and esophagus of rats. Int. J. Leg. Med. 106: p 142-144 1993.
17. Yamada K., Fukushima T., Mechanism of cytotoxicity of paraquat, II. organ specificity of paraquat-stimulated lipid peroxidation in the inner membrane of mitochondria. Exp. Toxic. Pathol. 45: p. 375-380, 1993.
18. Simon L. M., Nemcsok J. and Boross L., Studies on the effect of paraquat on glycogen mobilization in liver of common carp (Cyprinus carpio L.).Comp. Biochem. Physiol. Vol. 75C No 1 pp 167-169, Great Britain, 1983.
19. Polat F., Dere E. Paraquat'ın farelerin (Mus Musculus- swiss albino) farklı dokularında adenozin deaminaz aktiviteleri üzerine etkisi Türkish Journal of Biology 21, 3 317-328 1997