Neuroepithelial endocrine cells in the lung of Rana ridibunda an immunohistochemical study

Bu çalışma ile nöroepiteliyal endokrin hücreler Rana ridibunda akciğerinde ilk kez çalışıldı. Bu hücrelerin varlıkları endokrin işaretleyiciler kullanılarak immünohistokimyasal metodla ışık mikroskobu seviyesinde araştırıldı. Serotonin-, kalsitonin-, met-enkefalin- ve leu-enkefalin-immünoreaktif hücreler tek ya da yığınlar halinde solunum epiteli içinde gözlendi. Serotonin içeren yığınlar halindeki hücreler, çoğunlukla solunum havasının ilk karşılandığı yerler olan birincil kıvrımların genişlemiş apikal bölgelerindeki psödostratifiye epitelde, silli ve goblet hücrelerinin aralarında belirtendiler. Pnömositler arasındaki tek ve yığınlaşmış nöroepiteliyal endokrin hücreler, epitelin geri kalan kısmında kalsitonin, met-enkefalin ve leu-enkefalin immünoreaktivitesi gösterdiler. Tüm immünoreaktif hücrelerin akciğerdeki dağılımları, epiteldeki yerleşim yerleri ve morfolojik özellikleri dikkate alındığında, onların Rana ridibunda akciğerinin birkaç bölgesinde parakrin/endokrin yol ile farklı görevlere sahip olabilecekleri sonucuna varılmıştır.

Rana ridibunda' nın akciğerindeki nöroepiteliyal endokrin hücreler immünohistokimyasal bir çalışma

In this study, neuroepithelial endocrine cells were for the first time identified in the lung of Rana ridibunda. The presence of these cells was investigated using endocrine markers by the immunohistochemical method at the light microscopic level. Immunoreactive - cells containing serotonin, calcitonin, met-enkephalin and leu-enkephalin were observed alone or in clusters within the respiratory epithelium. In general, clustered - cells containing serotonin were detected among the ciliated cells and goblet cells of the pseudostratified epithelium on apical dilation of the primary septa where the inhaled - air is first met. Among pneumocytes, single and clustered neuroepithelial endocrine cells display calcitonin, met-enkephalin and leu-enkephalin immunoreactivity in the rest of the epithelium. All immunoreactive - cells have different functions in different regions of the lung in Rana ridibunda by the paracrine/endocrine pathway, taking into consideration their morphological properties, localization in the epithelium and distribution in the lung.

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