Rabiye TERZİ, Neslihan SARUHAN GÜLER, Nihal KUTLU ÇALIŞKAN, Asım KADIOĞLU

Lignification response for rolled leaves of Ctenanthe setosa under long-term drought stress

Leaf rolling is a dehydration avoidance mechanism for plants under drought stress. To understand how it affects the lignification process in response to long-term drought stress in Ctenanthe setosa plants that have the leaf-rolling mechanism, the enzymes in lignification were studied in unrolled leaves as a control and at 2 different leaf rolling indices at days 35 and 47 of the drought period. The results indicated that the activities of phenylalanine ammonia lyase, indole-3-acetic acid oxidase, soluble peroxidase, ionically wall-bound peroxidase, covalently wall-bound peroxidase, and polyphenol oxidase were increased during long-term drought stress. However, nitrate reductase activity was decreased while lignin content was increasing during the period of drought. Lignin content was positively correlated to activities of phenylalanine ammonia lyase, indole-3-acetic acid oxidase, soluble peroxidase, ionically wall-bound peroxidase, and polyphenol oxidase, but it was negatively correlated to activity of nitrate reductase. Furthermore, there was a positive correlation between leaf rolling and lignin content. The results indicate that the leaf-rolling process may be related to the lignification mechanism in alleviating damage from stress in Ctenanthe setosa under long-term drought stress.

Anahtar Kelimeler:

Key words: Ctenanthe setosa, enzyme activity, leaf rolling index, leaf water potential, lignification, long-term drought stress, relative water content

Lignification response for rolled leaves of Ctenanthe setosa under long-term drought stress

Keywords:

Key words: Ctenanthe setosa, enzyme activity, leaf rolling index, leaf water potential, lignification, long-term drought stress, relative water content,

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Beffa R, Martiz HV, Pilet PE (1990). In vitro oxidation of indole acetic acid by soluble auxin oxidases and peroxidases from maize roots. Plant Physiol 94: 485–491.
Bolwell GP, Davis DR, Gerrish C, Auh CK, Murphy TM (1998). Comparative biochemistry of the oxidative burst produced by rose and French bean cells reveals two distinct mechanisms. Plant Physiol 116: 1379–1385.
Bradford MM (1976). Rapid and sensitive method for the quantitation of microgram quantities protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
Cervilla LM, Rosales MA, Rubio-Wilhelmi MM, Sánchez-Rodríguez E, Blasco B, Ríos JJ (2009). Involvement of lignification and membrane permeability in the tomato root response to boron toxicity. Plant Sci 176: 545–552.
Chen LM, Lin CC, Kao CH (2000). Copper toxicity in rice seedlings: changes in antioxidative enzyme activities, H2O2 level and cell wall peroxidase activity in roots. Bot Bull Acad Sinica 41: 99–103.
Espin JC, Trujano MF, Tudelo J, García-Cánovas F (1995). Monophenolase activity of polyphenol oxidase from Haas avocado. J Agr Food Chem 45: 1091–1096.
Ferrer MA, Pedreño MA, Muñoz R, Ros Barceló A (1990). Oxidation of coniferyl alcohol by cell wall peroxidases at the expense of indole-3-acetic acid and O2. FEBS Lett 276: 127–130.
Fry SC (1986). Cross-linking of matrix polymers in the growing cell wall of angiosperms. Ann Rev Plant Physio 37: 165–186.
Fry SC (2004). Primary cell wall metabolism: tracking the careers of wall polymers in living plant cells. New Phytol 161: 641–675.
Havir EA, Reid PD, Marsh HV (1971). L-phenylalanine ammonia- lyase (maize): evidence for a common catalytic site for L-phenylalanine and L-tyrosine. Plant Physiol 48: 130–136.
Iiyama K, Wallis AFA (1990). Determination of lignin in herbaceous plants by an improved acetyl bromide procedure. J Sci Food Agr 51: 145–161.
Jouili H, El Ferjani E (2003). Changes in antioxidative and lignifying enzyme activities in sunflower roots (Helianthus annuus L.) stressed with copper excess. C R Biol 326: 639–644.
Kadioglu A, Terzi R (2007). A dehydration avoidance mechanism: leaf rolling. Bot Rev 73: 290–302.
Kalarani MK, Jeyakumar D (1998). Effect of nutrient and NAA spray on physiological changes in soybean. Indian J Plant Physi 38: 197–202.
Lee BR, Kim KY, Jung WJ, Avice JC, Ourry A, Kim TH (2007). Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.). J Exp Bot 58: 1271–1279.
Moura JCMS, Bonine CAV, Viana JOF, Dornelas MC, Mazzafera P (2010). Abiotic and biotic stresses and changes in the lignin content and composition in plants. J Integr Plant Biol 52: 360– 376.
Nakashima J, Awano T, Takebe K, Fujita M, Saiki H (1997). Immunocytochemical localization of phenylalanine ammonia- lyase and cinnamylalcohol dehydrogenase in differentiating tracheary elements derived from Zinnia mesophyll cells. Plant Cell Physiol 38: 113–123.
Nakayama T, Yonekura-Sakakibara K, Sato T, Kikuchi S, Fukui Y, Fukuchi-Mizutani M, Ueda T, Nakao M, Tanaka Y, Kusumi T et al. (2000). Aureusidin synthase: a polyphenol oxidase homolog responsible for flower coloration. Science 290: 1163–1166.
Pandolfini T, Gabbrielli R, Comparini C (1992). Nickel toxicity and peroxidase activity in seedlings of Triticum aestivum L. Plant Cell Environ 15: 719–725.
Pujari DS, Chandra SV (2002). Effect of salinity stress on growth, peroxidase and IAA oxidase activities in Vigna seedlings. Acta Physiol Plant 24: 435–439.
Rivero RM, Ruiz JM, García LR, López-Lefebre E, Sánchez E, Romero L (2001). Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Sci 160: 315–321.
Saruhan N, Terzi T, Sağlam A, Kadıoğlu A (2009). The relationship between leaf rolling and ascorbate-glutathione cycle enzymes in apoplastic and symplastic areas of Ctenanthe setosa subjected to drought stress. Biol Res 42: 315–326.
Saruhan Güler N, Sağlam A, Demiralay M, Kadıoğlu A (2012). Apoplastic and symplastic solutes concentrations contribute to osmotic adjustment in bean genotypes during drought stress. Turk J Biol 36: 151–160.
Schrader LE, Hageman RH (1967). Regulation of nitrate reductase activity in corn (Zea mays L.) seedlings by endogenous metabolites. Plant Physiol 42: 1750–1756.
Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld- Heyser R, Godbold OL, Polle A (2001). Cadmium-induced changes in antioxidative systems, H2O2 content, and differentiation in pine (Pinus sylvestris) roots. Plant Physiol 127: 887–892.
Shi ZY, Wang J, Wan XS, Shen GZ, Wang XQ, Zhang JL (2007). Over- expression of rice OsAGO7 gene induces upward curling of the leaf blade that enhanced erect-leaf habit. Planta 226: 99–108.
Stewart GR, Gracia CA, Hegarty EE, Specht RL (1990). Nitrate reductase activity and chlorophyll content in sun leaves of subtropical Australian closed-forest (rainforest) and open- forest communities. Oecologia 82: 544–551.
Valentines MC, Vilaplana R, Torres R, Usall J, Larrigaudiere C (2005). Specific roles of enzymatic browning and lignification in apple disease resistance. Postharvest Biol Tec 36: 227–234.
Yandow TS, Klein M (1986). Nitrate reductase of primary roots of red spruce seedling. Plant Physiol 81: 723–725.
Zarra I, Sánchez E, Queijeiro M, Peña J, Revilla G (1999). The cell wall stiffening mechanism in Pinus pinaster Aiton: regulation by apoplastic level of ascorbate and hydrogen peroxide. J Sci Food Agr 79: 416–420.