Işıl SEYİS BİLKAY, Şafak KARAKOÇ, Nilüfer AKSÖZ

Indole-3-acetic acid and gibberellic acid production in Aspergillus niger

The effects of incubation time, temperature, pH, and agitation on indole-3- acetic acid and gibberellic acid production in Aspergillus niger were studied. For indole-3-acetic acid production, 6 days of incubation at 25 °C and pH 6.0 was found to be optimum. Optimum conditions for gibberellic acid production were 12 days of incubation at 30 °C and pH 5.0. Agitation increased both indole-3-acetic acid and gibberellic acid production.

Anahtar Kelimeler:

Key words: Aspergillus niger, gibberellic acid, indole-3-acetic acid, phytohormone, plant growth regulator

Indole-3-acetic acid and gibberellic acid production in Aspergillus niger

Keywords:

Key words: Aspergillus niger, gibberellic acid, indole-3-acetic acid, phytohormone, plant growth regulator,

PDF

___

1. Bandelier S, Renaud R, Durand A. Production of gibberellic acid by fed batch solid-state fermentation in an aseptic pilot scale reactor. Process Biochem 32: 141-145, 1997.
2. Ma Z, Ge L, Lee ASY et al. Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. Anal Chim Acta 610: 274-281, 2008.
3. Gomi K, Matsuoka M. Gibberellin signalling pathway. Curr Opin Plant Biol 6: 489-493, 2003.
4. Boğa A, Binokay S, Sertdemir Y. The toxicity and teratogenicity of gibberellic acid (GA3) based on the frog embryo teratogenesis assay-Xenopus (FETAX). Turk J Biol 33: 181-188, 2009.
5. Nakamura A, Umemura I, Gomi K et al. Production and characterization of auxin-insensitive rice by overexpression of a mutagenized rice IAA protein. Plant J 46: 297-306, 2006.
6. Cihangir N, Aksöz N. Aspergillus niger'den gibberellik asit eldesi ve uygun fizyolojik koşulların saptanması. Doğa 17: 63-74, 1993.
7. Rademacher W. Gibberellin formation in microorganisms. Plant Growth Regul 15: 303-314, 1994.
8. Vardar Y. Bitki Fizyolojisine Giriş. Barış Yayınları. İzmir, Turkey; 1985.
9. Patten CL, Glick BR. Bacterial biosynthesis of indole-3-acetic acid. J Gen Microbiol 138: 377-381, 1992.
10. Bastian F, Cohen A, Piccoli P et al. Production of indole-3-acetic acid and gibberellins A1 and A3 by Acetobacter diazotrophicus and Herbaspirillum seropedicae in chemically defined culture. Plant Growth Regul 24: 7-11, 1998.
11. Bhattocharyya RN, Pati BR. Growth behaviour and indole acetic acid (IAA) production by a Rhizobium isolated from root nodules of Alysicarpus vaginalis DC. Acta Microbiol Immunol Hung 47: 41-51, 2000.
12. Ahmad F, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 163: 173-181, 2008.
13. Mahadevan A, Chandramohan D. Auxin production by the rice leaf blight fungus, Helminthosporium oryzae and other Helminthosporium. Ind Phylopath Soc Symp Bull 3: 91-96, 1966.
14. Cho KY, Sakurai A, Kamiya Y et al. Effects of the new plant growth retardants of quaternary ammonium iodides on gibberellin biosynthesis in Gibberella fujikuroi. Plant Cell Physiol 20: 25-81, 1979.
15. Bruckner B, Blechschmidt D. The gibberellin fermentation. Crit Rev Biotechnol 11: 163-192, 1991.
16. Kahlon SS, Mahlotra S. Production of gibberellic acid by fungal mycelium immobilized in sodium alginate. Enzyme Microb Tech 8: 613-616, 1986.
17. Yalçınkaya Y, Effects of some physiological conditions on indole-3-acetic acid production by Gibberella fujikuroi G5, MSc, Hacettepe University Institute of Science and Technology, 2007.
18. Gelmi C, Perez Correa R, Gonzalez M et al. Solid substrate cultivation of G. fujikuroi on an inert support. Process Biochem 35: 1227-1233, 2000.
19. Shukla R, Chand S, Srivastava AK. Batch kinetics and modeling of gibberellic acid production by Gibberella fujikuroi. Enzyme Microb Tech 36: 492-497, 2005.
20. Gopinathan S, Raman N. Indole-3-acetic acid production by ectomycorrhizal fungi. Indian J Exp Biol, 30: 142-143, 1992.
21. Gunasekaran M. Physiological studies on Phymatotrichum omnivorum IX, Synthesis of indole acetic acid in vitro. Microbios 22: 85-91, 1978.
22. Hasan HA. Gibberellin and auxin-indole production by plant root-fungi and their biosynthesis under salinity-calcium interaction. Acta Microbiol Immunol Hung 49: 105-118, 2002.
23. Gelmi C, Perez Correa R, Agosin E. Modelling Gibberella fujikuroi growth and GA3 production in solid-state fermentation. Process Biochem 37: 1033-1040, 2002.
24. Escamilla SE, Dendooven L, Magana PI et al. Optimization of GA3 production by immobilized G. fujikuroi mycelium in fluidized bioreactors. J Biotechnol 76: 147-155, 2000.
25. Prescott AG. A dilemma of dioxygenases. J Exp Biol 44: 849- 861, 1993.
26. Urrutia O, Hedden P, Rojas CM. Monooxygenases involved in GA12 and GA14 synthesis in Gibberella fujikuroi. Phytochemistry 56: 505-511, 2001.
27. Thuler DS, Floh EI, Handro W et al. Beijerinckia derxii releases plant growth regulators and amino acids in synthetic media independent of nitrogenase activity. J Appl Microbiol 95: 799- 806, 2003.
28. Cihangir N, Aksöz N, Bozcuk S. The interaction of microorganismal plant growth regulators. Tr J of Biology 20: 135-142, 1996.