Design and in vitro, in vivo evaluation of antioxidant bioadhesive gels for burn treatment

Design and in vitro, in vivo evaluation of antioxidant bioadhesive gels for burn treatment

Burn wounds are frequently encountered health problems, which need a new treatment approach especially in terms of good patient compliance. Availability of use of antioxidant agents and bio-adhesive gels in tissue healing can be an alternative as a new approach for wound healing. Antioxidant taurine containing bio-adhesive gels were prepared by using carbopol (CP) 940 and 934. Rheological and texture analyses were carried out on bio-adhesive gels for in vitro characterization. Wound model on Wistar rats was used to evaluate the in vivo evaluation of gels. Rheological and texture analyses showed that a carbopol bioadhesive gel has acceptable topically use dosage characteristics and in combination with Taurine it presented a successful wound healing effect via antioxidant parameters. In conclusion, bio-adhesive CP 940 (2%) gel containing 50 mM taurine could be promising in the treatment of burns by balancing oxidative stress.

___

  • Abdullahi A, Amini-Nik S, Jeschke MG (2014). Animal models in burn research. Cellular and Molecular Life Sciences 71 (17): 3241-55.
  • Ashkani-Esfahani S, Zarifi F, Asgari Q, Samadnejad AZ, Rafiee S et al. (2014). Taurine improves the wound healing process in cutaneous leishmaniasis in mice model, based on stereological parameters. Advanced Biomedical Research 3: 204.
  • Bonacucina G, Cespi M, Misici-Falzi M, Palmieri GF (2006). Rheological, adhesive and release characterization of semisolid Carbopol/tetraglycol systems. International Journal of Pharmaceutics 307: 129-140.
  • Bradford MM (1976). A Rapid and Sensitive Method for the Quantition of Microgram Quantities of Protein Utilizing the Princible of Protein-Dye Binding. Analytical Biochemistry 72: 248-251.
  • Çetinkalp Ş, Gökçe EH, Şimşir I, Tuncay Tanrıverdi S, Doğan F et al. (2021). Comparative Evaluation of Clinical Efficacy and Safety of Collagen Laminin-Based Dermal Matrix Combined with Resveratrol Microparticles (Dermalix) and Standard Wound Care for Diabetic Foot Ulcers. The International Journal of Lower Extremity Wounds 20 (3): 217-226.
  • D’Avignon LC, Hogan BK, Murray CK, Loo FL, Hospenthal DR et al. (2010). Contribution of bacterial and viral infections to attributable mortality in patients with severe burns: an autopsy series. Burns 36 (6): 773–779.
  • Deasy PB, Quigley KJ (1991). Rheological evaluation of deacetylated gellan gum (Gelrite) for pharmaceutical use. International Journal of Pharmaceutics 73: 117-123.
  • Değim Z, Celebi N, Sayan H, Babül A, Erdoğan D et al. (2002). An investigation on skin wound healing in mice with a taurinechitosan gel formulation. Amino Acids 22 (2): 187-198.
  • Dunnill C, Patton T, Brennan J, Barrett J, Dryden M et al. (2017). Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. International Wound Journal 14 (1): 89-96.
  • Fitzmaurice SD, Sivamani RK, Isseroff RR (2011). Antioxidant therapies for wound healing: a clinical guide to currently commercially available products. Skin Pharmacology and Physiology 24 (3): 113–126.
  • Gaikwad VL, Yadav VD, Dhavale RP, Choudhari PB, Jadhav SD (2012). Effect of Carbopol 934 and 940 on Fluconazole Release from Topical Gel Formulation: A Factorial Approach. Current Pharma Research 2 (2): 487-493.
  • Hoyos M, Guerrero JM, Perez-Cano R, Olivan J, Fabiani F et al. (2000). Serum cholesterol and lipid peroxidation are decreased by melatonin in diet-induced hyper-cholesterolemic rats. Journal of Pineal Research 28: 150-55.
  • Hosseini SV, Niknahad H, Fakhar N, Rezaianzadeh A, Mehrabani D (2011). The Healing Effect of Mixture of Honey, Putty, Vitriol and Olive oil in Pseudomonas aeroginosa Infected Burns in Experimental Rat Model. Asian Journal of Animal and Veterinary Advances 6: 572-579.
  • Jones DS, Lawlor MS, Woolfson AD (2003). Rheological and mucoadhesive characterization of polymeric systems composed of poly (methyvinylether-co-maleic anhydride) and poly(vinylpyrrolidone), designed as platforms for topical drug delivery. Journal of Pharmaceutical Sciences 92: 995-1007.
  • Jones DS, Woolfson AD, Brown AF (1997). Textural, viscoelastic and mocoadhesive properties of pharmaceutical gels composed of cellulose polymers. International Journal of Pharmaceutics 151: 223-233.
  • Karavana SY, Güneri P, Ertan G (2009). Benzydamine hydrochloride buccal bioadhesive gels designed for oral ulcers: Preparation, rheological,textural, mucoadhesive and release properties, Pharmaceutical Development and Technology 14 (6): 623–631.
  • Korkmaz E, Gokce EH, Ozer O (2013). Development and evaluation of coenzyme Q10 loaded solid lipid nanoparticle hydrogel for enhanced dermal delivery. Acta Pharmaceutica 63: 517–529.
  • Martin, P (1997). Wound Healing-Aiming for Perfect Skin Regeneration. Science 276: 75-84.
  • Mattson JP, Sun J, Murray DM, Poole DC (2002). Lipid peroxidation in the skeletal muscle of hamsters with emphysema. Pathophysiology 8: 215-21.
  • Niu X, Zheng S, Liu H, Li S (2018). Protective effects of taurine against inflammation, apoptosis, and oxidative stress in brain injury. Molecular Medicine Reports 18: 4516–4522.
  • Okatani Y, Wakatsuki A, Kaneda C (2000). Melatonin increases activities of glutathione peroxidase and superoxide dimutase in fetal rat brain. Journal of Pineal Research 28: 89-96.
  • Perioli L, Ambrogi V, Venezia Pagano C, Ricci M, Rossi C (2008). Chitosan and a modified chitosan as agents to improve performances of mucoadhesive vaginal gels. Colloids and Surfaces B: Biointerfaces 66: 141–145.
  • Poljsak B, Šuput D, Milisav I (2013). Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxidative Medicine and Cellular Longevity 956792.
  • Portugal M, Barak V, Ginsburg I, Kohen R (2007). Interplay among oxidants, antioxidants, and cytokines in skin disorders: Present status and future considerations. Biomedicine and Pharmacotherapy 61: 412-422.
  • Rowan MP, Cancio LC, Elster EA, Burmeister DM, Rose LF et al. (2015). Burn wound healing and treatment: review and advancements. Critical Care (London, England) 19: 243.
  • Salinas J, Drew G, Gallagher J, Cancio LC, Wolf SE et al. (2008). Closedloop and decision-assist resuscitation of burn patients. Journal of Trauma 64: 321-332. doi:10.1097/TA.0b013e31816bf4f7
  • Schuller-Levis GB, Park E (2003). Taurine: new implications for an old amino acid. FEMS Microbiology Letters 226 (2): 195-202.
  • Stamm A, Reimers K, Strauß S, Vogt P, Scheper T et al. (2016). In vitro wound healing assays – state of the art. BioNanoMaterials 17: 79-87.
  • Tiwari VK (2012). Burn wound: How it differs from other wounds? Indian Journal of Plastic Surgery 45 (2): 364–373.
  • Tan YT, Peh KK, Al-Hanbali O (2000). Effect of carbopol and Polyvinylpyrrolidone on the Mechanical, Rheological, and Release Properties of Bioadhesive Polyethylene Glycol Gels, American Association of Pharmaceutical Scientists 17 (3): E24. doi: 10.1208/pt010324
  • Wang JH, Redmond HP, Watson RW, Condron C, Bouchier–Hayes D (1996). The beneficial effect of taurine on the prevention of human endothelial cell death. Shock 6: 331–338.
  • White CE, Renz EM (2008). Advances in surgical care: management of severe burn injury. Critical Care Medicine 36 (7): S318– S324. doi:10.1097/CCM.0b013e31817e2d64
  • Wound Care for Diabetic Foot Ulcers. International Journal of Lower Extremity Wounds 20 (3): 217-226.
  • Zhang X, Kang X, Jin L, Bai J, Liu W et al. (2018). Stimulation of wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF). International Journal of Nanomedicine 13: 3897–3906.
  • Zatz JL, Kushla GP (1996). “Gels.”, In; Lieberaman HA, Riger MM, Banker GS, Pharmaceutical dosage forms: Disperse systems, Marcel Dekker, Volume 2: 399-421.