Chromosomal differentiation between populations of Clarias gariepinus (Burchell, 1822) from the Göksu Delta and Orontes River (Turkey)

The karyotype of Clarias gariepinus was described by means of G-banding, C-banding, Q-banding, and Ag-NORs (NORs) staining in this study. Samples were collected from 2 regions of Turkey: the Göksu Delta and Orontes River. Although samples from both regions had the same diploid chromosome number and fundamental arm number, (2n = 56, FN = 100), they had remarkable differences in karyotype formula and distribution of rDNA sites, according to silver nitrate staining and G-banding. While the karyotype formula for Orontes River samples was 24m + 10sm + 10st + 12a, the Göksu Delta samples' was 28m + 6sm + 10st + 12a. Comparative analysis showed that there was intraspecific variability in NORs and different NOR numbers in the 2 populations. The study's findings show that the Göksu Delta and Orontes River samples differed from each other.

Anahtar Kelimeler:

Key words: Clarias gariepinus, Cytogenetic analyses, Göksu Delta, Orontes River, Turkey

Chromosomal differentiation between populations of Clarias gariepinus (Burchell, 1822) from the Göksu Delta and Orontes River (Turkey)

Keywords:

Key words: Clarias gariepinus, Cytogenetic analyses, Göksu Delta, Orontes River, Turkey,

PDF

___

John B, Milkos GLG. Functional aspects of satellite DNA and heterochromatin. Int Rev Cytol 58: 1-114, 1979.
Howell WM, Black DA. Controlled silver staining of nucleolus organizer regions with a protective colloidal developer. 1 Step Method Experiential 36: 1014-1015, 1980.
Sumner AT. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75: 304-306, 1972.
Schmid M. Chromosomal banding an Amphibia IV. Differentiation of GC and AT rich regions in Anura. Chromosoma 77: 83-103, 1980.
Seabright MR. A rapid banding technique for human chromosomes. Lancet 2: 971-972, 1971.
Levan A, Fredga K, Sandberg AA. Nomenclature for centromer position on chromosomes. Hereditas 52: 201-220, 1964.
Ueda T, Naoi H. BrdU-4Na-EDTA-Giemsa band karyotypes of 3 small freshwater fish, Danio rerio, Oryzias latipes, and Rhodeus ocellatus. Genome 42: 531-535, 1999.
Hudson AP, Cuny G, Cortadas J et al. An analysis of fish genomes by density gradient centrifugation. Eur J Biochem 112: 203-210, 1980.
Medrano L, Bernardi G, Couturier J et al. Chromosome banding and genome compartmentalization in fishes. Chromosoma 96: 178-183, 1988.
Galettı PM, Cesar ACG, Venere PC. Heterochromatin and NORs variability in Leporinus Characiformes). Caryologia 44: 287-292, 1991. fish (Anostomidae,
Mantovani M, Abel LDS, Mestriner CA et al. Accentuated polymorphism of heterochromatin and nuclear organizer regions in Astyanax scabripinnis (Pisces, Characidae): Tools for understanding karyotypic evolution. Genetica 109: 161-168, 2000.
Ueda T, Naoi H, Arai R. Flexibility on the karyotype evolution in bitterlings (Pisces, Cyprinidae). Genetica 111: 423-432, 2001.
Ozouf-Costaz C, Foresti F. Fish cytogenetic research advances applications and perspectives. Netherlands Journal of Zoology 42: 277-290, 1992.
Sola LS, Bressanello A, Rossi R et al. A karyotype analysis of the genus Dicentrarchus by different staining techniques. J Fish Biol 43: 329-337, 1993.
Takai A, Ojima Y. Chromosomal Distribution o f C-banded Heterochromatin in Cyprinid Fishes Proc. Japan Acad 64, Ser. B. 49, 1988.
Thode G, Alvarez MC, Giles V et al. Chromosome complement C-banding and Ag-NOR location in Ophysurus serpens (Ophichthidae, Anguilliformes). Cytobios 43: 73-77, 1985.
Ueda T, Ojima Y. Differential chromosomal characteristics in the funa subspecies (Carassius) Proc Japan Acad 54B, 283-288, 1978.
Ojima Y, Takai A. Further cytogenetical studies on the origin of the gold-fish. Proc Jpn Acad 55B, 346-350, 1979.
Ueda T, Ojima Y. Karyotypes with C-banding patterns of two species in the genus Salvelinus of the family Salmonidae. Proc Japan Acad 59, 343-346, 1983.
Thorgard GH, Dısney JE. Chromosome preparation and analysis methods for fish biology. American Fisheries Society, Bethesda, Maryland, USA, 1990; 171-187.
Ergene S, Yalcın S, Karahan A. A Preliminary Study on Metric and Meristic Variation of Clarias lazera (Valeciennes, 1840) which are Living Two Distinct Localities, III. National Aquaculture Symposium, 10-12. June, Erzurum 1998; 569-575.
Belknap DF, Mart Y. Sea-level low stand in the eastern Mediterranean: Late Pleistocene coastal terraces offshore northern Israel. J Coast Res 15: 399-412, 1999.
Por FD. The Legacy of Tethys: An Aquatic Biogeography of the Levant, Kluwer Academic, Dordrecht/Boston; 1989.
Arndt A, Neer WV, Hellemans B et al. Roman trade relationships at Sagalassos (Turkey) elucidated by ancient DNA of fish remains. J Arch Sci 30: 1095-1105, 2003.
Geldiay R, Balık S. Freshwater Fishes of Turkey. Ege University Press, Bornova, İzmir, Turkey, 1996. pp: 410-411.