Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-L- histidine/alginate microbeads by platelet-rich plasma
Type 1 diabetes is clinically characterized as the loss of control of glucose homeostasis due to the reduced number of insulinproducing
cells. Long-term glycemic control after implantation could be maintained by preserving the cell viability and tissue-specific
functions during the process of microencapsulation. In this study, alginate solution was supplemented with platelet-rich plasma (PRP)
to improve the viability and preserve the cell functions during the encapsulation of a beta cell line (BRIN-BD11). Cell viability was
assessed and insulin secretion and insulin stimulation index were evaluated. The polymerization of alginate with PRP enhanced the
viability up to 61% in the alginate microbeads. PRP supplementation to the alginate composition not only increased the number of
viable cells by 1.95-fold, but the insulin secretion also improved by about 66%. The stimulation index, however, was not affected by the
PRP supplementation.
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- Ausländer D, Ausländer S, Charpin-El Hamri G, Sedlmayer F, Müller
M, Frey O, Hierlemann A, Stelling J, Fussenegger M (2014).
A synthetic multifunctional mammalian pH sensor and CO
2
transgene-control device. Mol Cell 55: 397-408.
- Bhujbal SV, de Haan B, Niclou SP, de Vos P (2014). A novel multilayer
immunoisolating encapsulation system overcoming protrusion
of cells. Sci Rep 4: 6856.
- Bilalis P, Tziveleka LA, Varlas S, Iatrou H (2016). pH-Sensitive
nanogates based on poly(L-histidine) for controlled drug
release from mesoporous silica nanoparticles. Polymer Chem
7: 1475-1485.
- Calafiore R, Basta G, Luca G, Lemmi A, Racanicchi L, Mancuso
F, Montanucci MP, Brunetti P (2006). Standard technical
procedures for microencapsulation of human islets for graft
into nonimmunosuppressed patients with type 1 diabetes
mellitus. Transplant Proc 38: 1156-1157.
- Coue G, Engbersen JFJ (2015). Cationic polymers for intracellular
delivery of proteins. In: Samal SK, Dubruel P, editors. Cationic
Polymers in Regenerative Medicine. 1st ed. Cambridge, UK:
Royal Society of Chemistry, pp. 370-376.
- Desai T, Shea LD (2017). Advances in islet encapsulation technologies.
Nat Rev Drug Discov 16: 338-350.
- de Vos P, Bucko M, Gemeiner P, Navrátil M, Svitel J, Faas M, Strand
BL, Skjak-Braek G, Morch YA, Vikartovská A et al. (2009).
Multiscale requirements for bioencapsulation in medicine and
biotechnology. Biomaterials 30: 2559-2570.
- Dohan Ehrenfest DM, Andia I, Zumstein MA, Zhang CQ, Pinto
NR, Bielecki T (2014). Classification of platelet concentrates
(Platelet-Rich Plasma-PRP, Platelet-Rich Fibrin-PRF) for
topical and infiltrative use in orthopedic and sports medicine:
current consensus, clinical implications and perspectives.
Muscles Ligaments Tendons J 4: 3-9.
- Duruksu G, Aciksari A (2018). Guiding the differentiation direction
of pancreatic islet derived stem cells by glycated collagen. Stem
Cells Int 2018: 6143081.
- Gao ZG, Lee DH, Kim DI, Bae YH (2005). Doxorubicin loaded pH-
sensitive micelle targeting acidic extracellular pH of human
ovarian A2780 tumor in mice. J Drug Target 13: 391-397.
- Horimizu M, Kawase T, Nakajima Y, Okuda K, Nagata M, Wolff
LF, Yoshie H (2013). An improved freeze-dried PRP-
coated biodegradable material suitable for connective tissue
regenerative therapy. Cryobiology 66: 223-232.
- Jacobs-Tulleneers-Thevissen D, Chintinne M, Ling Z, Gillard P,
Schoonjans L, Delvaux G, Strand BL, Gorus F, Keymeulen B,
Pipeleers D (2013). Beta Cell Therapy Consortium EU-FP7.
Sustained function of alginate-encapsulated human islet cell
implants in the peritoneal cavity of mice leading to a pilot
study in a type 1 diabetic patient. Diabetologia 56: 1605-1614.
- Kakudo N, Morimoto N, Kushida S, Ogawa T, Kusumoto K (2014).
Platelet-rich plasma releasate promotes angiogenesis in vitro
and in vivo. Med Mol Morphol 47: 83-89.
- Kushida S, Kakudo N, Morimoto N, Hara T, Ogawa T, Mitsui T,
Kusumoto K (2014). Platelet and growth factor concentrations
in activated platelet-rich plasma: a comparison of seven
commercial separation systems. J Artif Organs 17: 186-192.
- Lee ES, Na K, Bae YH (2003a). Polymeric micelle for tumor pH and
folate-mediated targeting. J Control Release 91: 103-113.
- Lee ES, Oh KT, Kim D, Youn YS, Bae YH (2007). Tumor pH-
responsive flower-like micelles of poly(L-lactic acid)-b-
poly(ethylene glycol)-b-poly(L-histidine). J Control Release
123: 19-26.
- Lee ES, Shin HJ, Na K, Bae YH (2003b). Poly(L-histidine)-PEG block
copolymer micelles and pH-induced destabilization. J Control
Release 90: 363-374.
- Man Y, Wang P, Guo Y, Xiang L, Yang Y, Qu Y, Gong P, Deng L (2012).
Angiogenic and osteogenic potential of platelet-rich plasma
and adipose-derived stem cell laden alginate microspheres.
Biomaterials 33: 8802-8811.
- Nagata MJ, Messora MR, Furlaneto FA, Fucini SE, Bosco AF, Garcia
VG, Deliberador TM, de Melo LG (2010). Effectiveness of two
methods for preparation of autologous platelet-rich plasma: an
experimental study in rabbits. Eur J Dent 4: 395-402.
- Okuda K, Tai H, Tanabe K, Suzuki H, Sato T, Kawase T, Saito Y, Wolff
LF, Yoshiex H (2005). Platelet-rich plasma combined with
a porous hydroxyapatite graft for the treatment of intrabony
periodontal defects in humans: a comparative controlled
clinical study. J Periodontol 76: 890-898.
- Orive G, Santos E, Pedraz JL, Hernández RM (2014). Application
of cell encapsulation for controlled delivery of biological
therapeutics. Adv Drug Deliv Rev 67: 3-14.
- Prokop A, Hunkeler D, DiMari S, Haralson MA, Wang TG (1998).
Water soluble polymers for immunoisolation I: Complex
coacervation and cytotoxicity. Adv Polym Sci 136: 1-51.
- Prüsse U, Bilancetti L, Bucko M, Bugarski B, Bukowski J, Gemeiner
P, Lewinska D, Manojlovic V, Massart B, Nastruzzi C et al.
(2008). Comparison of different technologies for alginate
beads production. Chemical Papers 62: 364-374.
- Robles L, Storrs R, Lamb M, Alexander M, Lakey JR (2014). Current
status of islet encapsulation. Cell Transplant 23: 1321-1348.
- Sarker B, Papageorgiou DG, Silva R, Zehnder T, Gul-E-Noor F,
Bertmer M, Kaschta J, Chrissafis K, Detsch R, Boccaccini AR
(2014). Fabrication of alginate-gelatin crosslinked hydrogel
microcapsules and evaluation of the microstructure and
physio-chemical properties. J Mater Chem B 2: 1470-1482.
- Strand BL, Ryan TL, In’t Veld P, Kulseng B, Rokstad AM, Skjak-Brek
G, Espevik T (2001). Poly-L-lysine induces fibrosis on alginate
microcapsules via the induction of cytokines. Cell Transplant
10: 263-275.
- van Schilfgaarde R, de Vos P (1999). Factors influencing the properties
and performance of microcapsules for immunoprotection of
pancreatic islets. J Mol Med (Berl) 77: 199-205.
- Wu H, Zhu L, Torchilin VP (2013). pH-sensitive poly(histidine)-
PEG/DSPE-PEG co-polymer micelles for cytosolic drug
delivery. Biomaterials 34: 1213-1222.
- Zimmermann U, Cramer H, Jork A, Thurmer F, Zimmermann H,
Fuhr G, Hasse C, Rothmund M (2001). Microencapsulation-
based cell therapy. In: Rehm HJ, Reed G, editors. Biotechnology:
Special Processes. 1st ed. Weinheim, Germany: Wiley Press,
pp. 547-571.