Use of chemical mutagens for production of inactive pollen grains, embryo rescue, and morphological changes in cucumber
Application of chemical mutagens as important factors is very practical for successful haploidization techniques in Cucurbitaceae through pollen grain deactivation and then immature embryo rescue. The present research investigated the effects of genotype, male flower age, receptor plant, and chemical mutagenesis (NaN3 and colchicine) on pollen grain deactivation as well as seed production (number of total, full, half-full, and empty seeds) and morphological traits. Moreover, the effects of different plant growth regulators were tested on embryo cultures. The effects of different factors (mutagen, genotype, flower age) were investigated on plant regeneration from immature embryos that were inspected from half-full and empty seeds. Based on this study, some mutagenic treatments (0.005 colchicine and 0.0012 NaN3) led to the highest values of morphological and fruit yield traits in control plants, while 0.005 and 0.025 NaN3 led to reduced values of these traits. Among the genotypes, NBDC3 showed the highest number of different types of seeds. Among different chemical mutagens, NaN3 (0.0012 mg/L) and colchicine (0.005 mg/L) produced the greatest effects on seed production traits. Moreover, the highest values for all seed-related traits were recorded for crossing with fresh male flowers, whereas crossing with old male flowers contributed to the decline of seed-related traits. Mutagen-treated plants showed the highest empty seed numbers. Furthermore, the highest total and half-full seed numbers were obtained using 0.0012 and 0.005 mg/L NaN3 and colchicine. Results revealed that the highest and lowest regeneration percentages (66.67% and 26.67%) belonged to the media containing BAP+Kin+IBA (2+1+0.5) mg/L and BAP+Kin+IBA+NAA (2+1+0.5+0.1) mg/L, respectively. These media were used for immature embryo culture. Finally, genotypes NBDC1 and NBDC3 treated with 0.005 colchicine and 0.012 NaN3 showed higher frequencies of plant regeneration from immature embryos extracted from half-full and empty seeds.
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- Abubakar A, Falusi AO, Daudu OAY, Oluwajobi AO, Dangana MC,
Abejide DR (2015). Mutagenic effects of sodium azide and fast
neutron irradiation on the cytological parameters of M2 Lagos
spinach (
Celosia argentea
var
cristata
L.). World J Agri Res 3:
107-112.
- Adebola MO (2013). Mutagenic effects of sodium azide (NaN
3
)
on morphological characteristics of tomato (
Lycopersicum
esculentum
). Research Journal of Science and IT Management
20: 1-5.
- Al-Qurainy F, Khan S (2009). Mutagenic effects of sodium azide and
its application in crop improvement. World Appl Sci J 6: 1589-
1601.
Baktemur G, Taskin H, Buyukalaca S (2013). Comparison of different
methods for separation of haploid embryo induced through
irradiated pollen and their economic analysis in melon
(
Cucumis melo
var.
inodorus
). Scientific World J 2013: 1-7.
- Benjak A, Ercisli S, Vokurka A, Maletic E, Pejic I (2005). Genetic
relationships among grapevine cultivars native to Croatia,
Greece, and Turkey. Vitis 44: 73-77.
Cain DW, Emershad RL, Tarailo RE (1983). In-ovulo embryo culture
and seedling development of seeded and seedless grapes (
Vitis
vinifera
L.). Vitis 22: 9-14.
- Canan I, Gundogdu M, Seday U, Oluk CA, Karasahin Z, Eroglu EC,
Yazici E, Unlu M (2016). Determination of antioxidant, total
phenolic, total carotenoid, lycopene, ascorbic acid, and sugar
contents of
Citrus
species and mandarin hybrids. Turk J Agric
For 40: 894-899.
- Claveria E, Garcia-Mas J, Dolcet-Sanjuan R (2005). Optimization
of cucumber doubled haploid line production using in vitro
rescue of in-vivo induced parthenogenic embryos. J Amer Soc
Hort Sci 130: 555-560.
- Divanli-Türkan A, Khawar KM, Çiftçi CY, Özcan S (2006). Effects
of mutagenic sodium azide (NaN
3
) on in vitro development of
four peas (
Pisum sativum
L.) cultivars. Int J Agri Biol 8: 349-
351.
- Ercisli S, Tosun M, Duralija B, Voca S, Sengul M, Turan M (2010).
Phytochemical content of some black (
Morus nigra
L.) and
purple (
Morus rubra
L.) mulberry genotypes. Food Technol
Biotechnol 48: 102-106.
- Gemes-Juhasz A, Balogh P, Ferenczy A, Kristof Z (2002) Effect of
optimum stage of female gametophyte in cucumber (
Cucumis
sativus L
.). Plant Cell Rep 21: 105-111.
- Grozeva S, Velkov N (2014). In vitro plant regeneration of two
cucumbers (
Cucumis sativum
L.) genotypes: effects of explant
types and culture medium. Genetika 46: 485-493.
- Hazem F, Golabadi M (2016). Assessment of the viability of pollen
grain in different treated flowers of cucumbers (
Cucumis
sativus
L.). In: Proceedings of 2nd International and 14th
Iranian Genetics Congress;, 21–23 May 2007; Tehran, Iran.
Hricova A, Fejer J, Libiakova G, Szabova M, Gazo J, Gajdosova
A (2016). Characterization of phenotypic and nutritional
properties of valuable
Amaranthus cruentus
L. mutants. Turk
J Agric For 40: 761-771.
- Kurtar ES (2009). Influence of gamma irradiation on pollen viability,
germination ability, and fruit and seed-set of pumpkin and
winter squash. Afr J Biotechnol 8: 6918-6926.
- Li GR, Ji W, Wang G, Zhang JX (2014). An improved embryo-rescue
protocol for hybrid progeny from seedless
Vitis vinifera
grapes
× wild Chinese
Vitis
species. In Vitro Cell Dev Biol Plant 50:
110-120.
- Li J, Wang X, Wang X, Wang Y (2015). Embryo rescue technique and
its applications for seedless breeding in grape. Plant Cell Tiss
Organ Cult 120: 861-880.
- Lotfi M, Alan AR, Henning MJ, Jahn M, Earle ED (2003). Production
of haploid and doubled haploid plants of melon (
Cucumis melo
L.) for use in breeding for multiple virus resistance. Plant Cell
Rep 21: 1121-1128.
- Murashige T, Skoog F (1962). A revised medium for rapid growth
and bioassays with tobacco tissue culture. Physiol Plant 15:
473-497.
- Navratilova B, Skalova D, Ondrej V, Kitner M, Lebeda A (2011).
Biotechnological methods utilized in Cucumis research. Hort
Sci 38: 150-158.
- Nunez-Palenius HG, Ramirez-Malagon R, Ochoa-Alejo N (2011).
Muskmelon embryo rescue techniques using in vitro embryo
culture. Methods Mol Biol 710: 107-115.
- Reed MS (2005). Embryo rescue. In: Trigiano RN, Gray DJ, editors.
Plant Development and Biotechnology. Boca Raton, FL, USA:
CRC Press, pp. 235-239.
- Rosell R, Sauco VG, Herrero M (2006). Pollen germination as
affected by pollen age in cherimoya. Scientia Hort 109: 97-100.
- Satpute RA, Fultambkar RV (2012). Effect of mutagenesis on
germination, survival and pollen sterility in M1 generation
of soybean [
Glycine max
(L.) Merill]. International Journal of
Recent Trends in Science and Technology 2: 30-32.
- Sauton A, Dumas-Vaulx R (1987). Obtention de plantes haploides
chez le melon (
cucumis melo
L.) par gynogenese induite par du
pollen irradie. Agronomy 7: 141-148 (in French).
- Zorenc Z, Veberic R, Stampar F, Koron D, Mikulic-Petkovsek
M (2016). Changes in berry quality of northern highbush
blueberry (
Vaccinium corymbosum
L.) during the harvest
season. Turk J Agric For 40: 855-867.