Rooting behaviour, polyphenol oxidase activity, and biochemical changes in grape rootstocks at different growth stages

Four grape rootstocks belonging to different Vitis species were planted in September. Rooting behaviour, polyphenol oxidase (PPO; EC 1.14.18.1) activity and biochemical parameters were studied at different growth stages after planting the cuttings in polythene bags. Significant differences were recorded for rooting success among the different rootstocks, with the maximum sprouting percentage determined in the Freedom rootstock. The highest variation in PPO activity was also recorded in Freedom. High PPO activity was recorded in rootstock 140 Ru, while the highest root length was recorded in Dog Ridge. In the rootstock 110 R, the PPO activity was the lowest during the initial stage, though it increased up to 60 days after planting (DAP) and was then reduced up to 90 DAP. Increases in the number of rooting primordials were recorded in all the different rootstocks at different stages of growth. A higher number of rooting primordials was recorded in Freedom, and it was followed by 110 R. The present study suggests the differences in the rooting behaviour of the different rootstocks are based on PPO activity at regular time intervals up to 90 DAP.

Rooting behaviour, polyphenol oxidase activity, and biochemical changes in grape rootstocks at diff erent growth stages

Four grape rootstocks belonging to different Vitis species were planted in September. Rooting behaviour, polyphenol oxidase (PPO; EC 1.14.18.1) activity and biochemical parameters were studied at different growth stages after planting the cuttings in polythene bags. Significant differences were recorded for rooting success among the different rootstocks, with the maximum sprouting percentage determined in the Freedom rootstock. The highest variation in PPO activity was also recorded in Freedom. High PPO activity was recorded in rootstock 140 Ru, while the highest root length was recorded in Dog Ridge. In the rootstock 110 R, the PPO activity was the lowest during the initial stage, though it increased up to 60 days after planting (DAP) and was then reduced up to 90 DAP. Increases in the number of rooting primordials were recorded in all the different rootstocks at different stages of growth. A higher number of rooting primordials was recorded in Freedom, and it was followed by 110 R. The present study suggests the differences in the rooting behaviour of the different rootstocks are based on PPO activity at regular time intervals up to 90 DAP.

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  • Altman A Wareing PF (1975) Th e eff ect of IAA on sugar accumulation and basipetal transport of 14C-labelled assimilates in relation to root formation in Phaseolus vulgaris cutting. Physiol Plant 33: 32-38.
  • Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiol 24: 1-15.
  • Coban H (2007) Determination of polyphenol oxidase activity during rooting in cuttings of some grape varieties (Vitis vinifera L). Asian J Chem 19: 4020-4024.
  • Dalet F, Cornu D (1998) Lignifi cation levels and peroxidase activity during in vitro rooting of Prunus avium. Can J Bot 67: 2192- 2186.
  • De Klerk GJ, Ter Brugge J (1992) Factors aff ecting adventitious root formation in microcuttings of Malus. Agron 12: 747-755
  • Esterbaner H, Schwarzl E, Hayn M (1977) A rapid assay for catechol oxidase and laccase using 2-nitro-5-thio benzoic acid. Analy Biochem 77: 486-494.
  • Gonazales R, Sanchez R, Rodriguez R, (1991) Ethylene in relation to proteins, peroxidase and polyphenol oxidase activities during rooting in hazelnut cotyledons. Physiol Plant 83: 611-620.
  • Hahlbrock K, Griesbach H (1979) Enzymatic controls in the biosynthesis of lignin and fl avonoids. Annu. Rev. Plant Physiol 30: 105-136.
  • Haissig BE, Davis TD (1994) A historical evaluation of adventitious rooting research to 1993. In: Biology of Adventitious Root Formation (Eds. TD Davis, BE Haissig). Plenum Press, NY, pp. 275-331.
  • Hartmann HT, Kester DE, Davies FT (1993) Plant propagation - principles and practices. Prentice Hall, New Delhi, India.
  • Hedge JE, Hofreiter BT (1962) In: Carbohydrate Chemistry 17 (Eds. Whistler RL and Be Miller JN) Academic Press, New York.
  • Levitt J (1980) Responses of Plants to Environmental Stresses, 2nd ed. Academic Press, NY, USA.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-Phenol reagents. J Biol Chem 193: 265-275.
  • Miller GL (1972) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31: 426-428.
  • Moe R, Anderson AS (1988) Stock plant environmental and subsequent adventitious rooting. In: Adventitious root formation cuttings (Eds. Davis TD, Haissig BE, Sankhla AN). Dioscorides Press, Portland, pp. 214-234.
  • Okoro OO, Grace J (1976) Th e physiology of rooting Populus cuttings: I. Carbohydrates and photosynthesis. Physiol Plant 36: 133-138.
  • Ozyigit II, Kahramanz MV, Ecranz O (2007) Relationship between explants age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.). Afr J Biotechnol 6: 3-8.
  • Prasad R (1989) Eff ect of nonyl phenol adjuvant on macrophytes. In: Adjuvant and agrochemicals: mode of action and physiology activity (Ed. PNP Chow). CRC Press, Boca Raton FL. Chap 6. Rios DG, Sanchez O, Gea ME, Revilla MA, Rodrigues R (1997) Rooting response in relation to PO, PPO, IIA O activities in walnut explants. In: Proceedings of III International Walnut Congress, Alcobaça, Portugal, pp. 235-442.
  • Sadhu MK, Bose S (1982) Propagation of Tropical and Subtropical Horticultural Crops. In: Proceedings of 21st Horticultural Congress, Hamburg, Germany p. 1379.
  • Sakai A, Larcher W (1987) Frost survival of plants: responses and adaptation to freezing stress. Springer-Verlag, Germany, p. 321.
  • Satisha J, Ramteke SD, Karibasappa GS (2007) Physiological and biochemical characterisation of grape rootstocks. South Afr J Enol Vitic 28: 163-168.
  • Satisha J, Raveendran P, Rokade ND (2008) Changes in polyphenol oxidase activity during rooting of hardwood cuttings in three grape rootstocks under Indian conditions. South Afr J Enol Vitic 29: 98-101.
  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. Am J Enol Vitic 16: 144-158.
  • Sturve DK (1981) Th e relation between carbohydrates, nitrogen and rooting of stem cuttings. Plant Propagat 27: 6-7.
  • Th omas P, Ravindra MB (1999) Shoot tip culture in mango: infl uence of medium, genotype, explants factors, season and decontamination treatments on phenolic exudation, explants survival and axenic culture establishment. J Hort Sci 72: 713- 722.
  • Tung W, Newton RJ (2004) Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginia pine (Pinus virginiana Mill). Plant Sci 167: 621-628.
  • Veierskow B (1988) Relations between carbohydrates and adventitious root formation. In: Adventitious root formation in cuttings (Eds. TD Davis, BE Haissig, AN Sankhla). Dioscorides Press, Portland, pp. 70-78.
  • Veierskov B, Anderson AS, Stumann BM, Henningsen KW (1982) Dynamics of extractable carbohydrates in Pisum cuttings. II. Carbohydrate content and photosynthesis of pea cuttings in relation to irradiance and stock plant temperature and genotype. Physiol Plant 55: 174-178.
  • White R (1994) Environmentally persistent alkylphenolic compounds are estrogenic. Endocrinol 135: 175-182.
  • Zachariakis M, Tzorakakis E, Kritsotakis I, Siminis CI, Manios V (2001) Humic substances stimulate plant growth and nutrient accumulation in grapevine rootstocks. International Symposium on Composting of Organic Matter. pp. 193-202.
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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