Isolation and characterization of culturable endophytic plant growth-promoting Bacillus species from Mentha longifolia L.

Isolation and characterization of culturable endophytic plant growth-promoting Bacillus species from Mentha longifolia L.

Plant growth-promoting bacteria is one of the most popular promising alternative fertilizers for sustainable agriculture. Hence, it is preferred by agriculturalists today due to its nontoxic, eco-friendly, and affordable costs as well as its benefits on agricultural products. Among these microorganisms, Bacillus is a widely known and investigated genus in rhizosphere of plenty of agricultural products. In the current study, endophytic Bacillus strains were isolated from the root, stem, and leaves of healthy Mentha longifolia L. in Palandöken Mountain, Erzurum-Turkey and evaluated their plant growth promoting parameters such as nitrogen fixation, phosphate solubilization, IAA, siderophore, ACC deaminase, HCN production, and ammonia potential. According to the results, 12 Bacillus strains showed multiple plant growth promoting properties (PGPs). Moreover, molecular identification of the potential PGP Bacillus strains was carried out by using PCR with the universal primers for 16S rRNA gene region. These strains were containing 3 Bacillus sp. (ML7, ML17 and ML46), 3 Bacillus simplex (ML12, ML25 and ML43), 2 Bacillus megaterium (ML36, ML61), 1 Bacillus muralis (ML49), 1 Bacillus aryabhattai (ML55), 1 Bacillus pumilus (ML59), and 1 Bacillus toyonensis (ML63). In conclusion, these results demonstrated that endophytic Bacillus species from native medicinal plants have huge potential for being utilized as promising natural plant growthpromoter as biofertilizers, biostimulants, and bioprotectants in sustainable crop production due to their beneficial PGPs.

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  • Abbas SR, Nisar M (2020). Herbal significance of horse mint (Mentha longifolia) a review. Journal of biotechnological sciences 8: 138-143.
  • Aeron A, Khare E, Jha CK, Meena VS, Aziz SMA et al. (2020). Revisiting the plant growth-promoting rhizobacteria: lessons from the past and objectives for the future. Archive of Microbiology 202: 665-676. doi: 10.1007/s00203-019-01779-w
  • Ahmed EA, Hassan EA, El Togby KMK, Ramadan EM (2014). Evaluation of rhizobacteria of some medicinal plants for plant growth promotion and biological control. Ann Agric Sci 59: 273–280. doi: 10.1016/j.aoas.2014.11.016
  • Akinsanya MA, Goh JK, Lim SP, Ting ASY (2015). Metagenomics study of endophytic bacteria in Aloe vera using next-generation technology. Genom Data 6: 159–163. doi: 10.1016/j.gdata.2015.09.004
  • Alaylar B, Güllüce M, Karadayi G, Karadayi M (2018). Isolation of PGPR strains with phosphate solubilizing activity from Erzurum and their molecular evaluation by using newly designed specific primer for pqqB gene. IJSER 9: 103–106. doi: 10.1007/ s00203-019-01779-w
  • Alaylar B, Güllüce M, Karadayi M, Isaoglu M (2019). Rapid detection of phosphate-solubilizing bacteria from agricultural areas in Erzurum. Current Microbiology 76: 804–809. doi: 10.1007/ s00284-019-01688-7
  • Alaylar B, Egamberdieva D, Gulluce M, Karadayi M, Arora NK (2020a). Integration of molecular tools in microbial phosphate solubilization research in agriculture perspective. World Journal of Microbiolgy and Biotechnology 36: 1-12. doi: 10.1007/ s11274-020-02870-x
  • Alaylar B, Gulluce M, Karadayi M (2020b). Detection of the nifh gene in nitrogen fixing bacteria from agricultural areas in Erzurum. Fresenius Environmental Bulletin 29: 809–814.
  • Al-Fredan M, Al-Fadal S (2019). Effects of nitrogen fertilizer rate on the essential oil content of Mentha longifolia, Mentha microphyta and Ocimum basilicum plants Journal of Scientific Research and Studies 6: 5-10.
  • Alkahtani MDF, Fouda A, Attia KA, Al-Qtaibi F, Eid AM et al. (2020). Isolation and characterization of plant growth promoting endophytic bacteria from desert plants and their application as bioinoculants for sustainable agriculture. Agronomy 10: 1-18. doi: 10.3390/agronomy10091325
  • Alsafar MS, Al-Hassan YM (2009). Effect of nitrogen and phosphorus fertilizers on growth and oil yield of indigenous mint (Mentha longifolia L.). Biotechnology 8: 380-384. doi: 10.3923/ biotech.2009.380.384
  • Ansary WR, Prince FRK, Haque E, Sultana F, West HM et al. (2018). Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth. Zeitschrift für Naturforschung C 1:1-10. doi: 10.1515/ znc-2018-0002
  • Anwar A, Abbas A, Mehmood T, Gilani AH, Rehman N (2019). Mentha: A genus rich in vital nutra-pharmaceuticals–A review. Phytotherapy Research 33: 2548–2570. doi: 10.1002/ptr.6423
  • Bakker A, Schippers B (1987). Microbial cyanides production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biology and Biochemistry 19: 451–457. doi: 10.1016/0038- 0717(87)90037-X
  • Borriss R (2011). Use of plant-associated Bacillus strains as biofertilizers and biocontrol agents in agriculture. In: Maheshwari D (editor). Bacteria in Agrobiology: Plant growth responses. Berlin, Germany: Springer, Heidelberg. pp. 41-46.
  • Dong Z, McCully ME, Canny MJ (1997). Does Acetobacter diazotrophicus live and move in the xylem of sugarcane stems? Anatomical and physiological data. Annals of Botany 80: 147–158.
  • Dubey A, Malla MA, Kumar A, Dayananda S, Khan ML (2020). Plants endophytes: unveiling hidden agenda for bioprospecting toward sustainable agriculture. Critical Reviews in Biotechnology 40: 1210-1231. doi: 10.1080/07388551.2020.1808584
  • Dung JKS, Schroeder BK, Johnson DA (2010). Evaluation of Verticillium wilt resistance in Mentha arvensis and M. longifolia genotypes. Plant Disease 94: 1255-1260. doi: 10.1094/PDIS-01- 10-0062
  • Ebrahimia A, Asghariana S, Habibianb S (2010). Antimicrobial activities of isolated endophytes from some Iranian native medicinal plants. Iranian Journal of Pharmaceutical Science 6: 217–222.
  • Egamberdieva D, Jaima JA, Teixeira DS (2015). Medicinal plants and PGPR: A new frontier for phytochemicals. In: Egamberdieva D, Teixeira DS (editors). Plant-growth-promoting rhizobacteria (PGPR) and medicinal plants. Berlin, Germany: Springer: Berlin, pp. 287–303.
  • Egamberdieava D, Shurigin V, Alaylar B, Wirth S, Bellingrath-Kimura SD (2020). Bacterial endophytes from horseradish (Armoracia rusticana G. Gaertn.,B. Mey.&Scherb.) with antimicrobial efficacy against pathogens. Plant Soil and Environment 66: 309-316. doi: 10.17221/137/2020-PSE
  • El-Deeb B, Fayez K, Gherbawy Y (2013). Isolation and characterization of endophytic bacteria from Plectranthus tenuiflorus medicinal plant in Saudi Arabia desert and their antimicrobial activities. Journal of Plant Interaction 8: 56–64. doi: 10.1080/17429145.2012.680077
  • Farid K, Zafari D, Soleisman MJ, Bagherabadi S (2020). First report of Nigrospora oryzae causing brown leaf spot on Mentha spicata. Journal of Plant Pathology 102: 1281. doi: 10.1007/s42161- 020-00562-x
  • Farzaei MH, Bahramsoltani R, Ghobadi A, Farzaei F, Najafi F (2017). Pharmacological activity of Mentha longifolia and its phytoconstituents. Journal of Traditional Chinese Medicine 37: 710-720. doi: 10.1016/S0254-6272(17)30327-8
  • Golinska P, Wypij M, Agarkar G, Rathod D, Dahm H et al. (2015). Endophytic actinobacteria of medicinal plants: diversity and bioactivity. Antonie van Leeuwenhoek 108: 267-289. doi: 10.1007/s10482-015-0502-7
  • Gupta RS, Patel S, Saini N, Chen S (2020). Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the subtilis and cereus clades of species. International Journal of Systematic and Evolutionary Microbiology 70: 5753–5798. doi: 10.1099/ijsem.0.004475
  • Hajlaoui H, Snoussi M, Ben Jannet H, Mighri Z, Bakhrouf A (2008). Comparison of chemical composition and antimicrobial activities of Mentha longifolia L. ssp. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid). Annals of Microbiology 58: 513-520. doi: 10.1007/BF03175551
  • Hanafy DM, Burrows GE, Prenzler PD, Hill AH (2020). Potential role of phenolic extracts of Mentha in managing oxidative stress and Alzheimer’s disease. Antioxidants 9: 1-24. doi: 10.3390/antiox9070631
  • Harrison JG, Griffin EA (2020). The diversity and distribution of endophytes across biomes, plant phylogeny and host tissues: how far have we come and where do we go from here? Environtal Microbiology 22: 2107-2123. doi: 10.1111/1462-2920.14968
  • Huang CM, Chen WC, Lin SH, Wang YN, Shen FT (2019). Exploration of root-associated bacteria from the medicinal plant Platycodon grandiflorum. Microbes Environment 4: 413-420. doi: 10.1264/jsme2.ME19030
  • Jensen HL (1942). Pro Line Soc. NSW 57: 205-212.
  • Kaki AA, Chaouche NK, Dehimat L, Milet A, Youcef-Ali M et al. (2013). Biocontrol and plant growth promotion characterization of Bacillus species isolated from Calendula officinalis rhizosphere. Indian Journal of Microbiology 53: 447-452. doi: 10.1007/s12088-013-0395-y
  • Karla A, Singh HB, Pandey R, Samad A, Patra NK et al. (2005). Diseases in mint: Causal organisms, distribution, and control measures. Journal of Herbs, Spices and Medicinal Plants 11: 71-91. doi: 10.1300/J044v11n01_03
  • Karthiekeyan B, Joe MN, Jaleel CA, Deiveekasundaram M (2010). Effect of root inoculation with plant growth promoting rhizobacteria (PGPR) on plant growth, alkaloid content and nutrient control of Catharanthus roseus (L.) G. Don. Nature and Croatica 19: 205-2012.
  • Kesdek M, Kordali S, Bozhuyuk AU, Gudek M (2020). Larvicidal effect of Achillea biebersteinii Afan. (Asteraceae) essential oil against larvae of pine processionary moth, Thaumetopoea pityocampa (Denis & Schiffermüller, 1775) (Lepidoptera: Notodontidae). Turkish Journal of Agriculture and Forestry 44: 451-460.
  • Khan AL, Gilani SA, Waqas M, Al-Hosni K, Al-Khiziri S et al. (2017). Endophytes from medicinal plants and their potential for producing indole acetic acid, improving seed germination and mitigating oxidative stress. Journal of Zhejiang UniversityScience B 18: 125-137. doi: 10.1631/jzus.B1500271
  • Li L, Mohamad OAA, Ma J, Friel AD, Su Y (2008). Synergistic plant– microbe interactions between endophytic bacterial communities and the medicinal plant Glycyrrhiza uralensis F. Antonie van Leeuwenhoek 111: 1735–1748. doi: 10.1007/s10482-018- 1062-4
  • Lolloo R, Maharaih D, Görgens J, Gardiner NA (2010). Downstream process for production of a viable and stable Bacillus cereus aquaculture biological agent. Applied Microbiology and Biotechnology 86: 499–508. doi: 10.1007/s00253-009-2294-z
  • Louden BC, Haarmann D, Lynn AM (2011). Use of blue agar CAS assay for siderophore detection. Journal of Microbiology and Biology Education 12: 51-53. doi: 10.1128/jmbe.v12i1.249
  • McCully ME (2001). Niches for bacterial endophytes in crop plants: a plant biologist’s view. Australian Journal of Plant Physiology 28: 983–990. doi: 10.1071/PP01101
  • Mikaili P, Mojaverrostami S, Moloudizargari M, Aghajanshakeri S (2013). Pharmacological and therapeutic effects of Mentha longifolia L. and its main constituent, menthol. Ancient Science of Life 33: 131-138. doi:10.4103/0257-7941.139059.
  • Mollova S, Fidan H, Antonova D, Bozhilov D, Stanev S et al. (2020). Chemical composition and antimicrobial and antioxidant activity of Helichrysum italicum (Roth) G. Don subspecies essential oils. Turkish Journal of Agriculture and Forestry 44: 371-378.
  • Murugappan RM, Begum SB, Roobia RR (2013). Symbiotic influence of endophytic Bacillus pumilus on growth promotion and probiotic potential of the medicinal plant Ocimum sanctum. Symbiosis 60: 91–99. doi: 10.1007/s13199-013-0244-0
  • Nema R, Khare S, Jain P, Pradha A, Gupta A et al. (2013). Natural products potential and scope for modern cancer research. American Journal of Plant Sciences 4: 1270–1277. doi: 10.4236/ ajps.2013.46157
  • Nnzeru LR, Ntushelo K, Mudau FN (2017). Prevalence of Bacillus in the interior tissues of Monsonia burkeana and other medicinal plants in South Africa. South African Journal of Botany 113: 19–22. doi: 10.1016/j.sajb.2017.07.012
  • Orhan F (2016). Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat (Triticum aestivum). Brazilian Journal of Microbiology 47: 621-627. doi: 10.1016/j.bjm.2016.04.001
  • Panchami PS, Thanuja KG, Karthikeyan S (2020). Isolation and characterization of indigenous plant growth-promoting rhizobacteria (PGPR) from Cardamom rhizosphere. Current Microbiology 77: 2693-2981. doi: 10.1007/s00284-020-02116-x
  • Patel S, Gupta RS (2020). A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov. International Journal of Systematic and Evalutionary Microbiology 70: 406–438. doi: 10.1099/ijsem.0.003775
  • Patti F, Palmioli A, Vitalini S, Bertazza L, Redaelli M et al. (2020). Anticancer effects of wild mountain Mentha longifolia extract in adrenocortical tumor cell models. Frontiers in Pharmacology 10: 1-11. doi: 10.3389/fphar.2019.01647
  • Penrose DM, Glick BR (2003). Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiologia Plantarum 118: 10–15. doi: 10.1034/j.1399-3054.2003.00086.x
  • Pikovskaya RI (1948). Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Mikrobiologiya 17: 362–370.
  • Preveena J, Bhore SJ (2013). Identification of bacterial endophytes associated with traditional medicinal plant Tridax procumbens Linn. Ancient Science of Life 32: 173–177. doi: 10.4103/0257- 7941.123002
  • Rana KL, Kour D, Kaur T, Devi R, Yadav AN et al. (2020). Endophytic microbes: biodiversity, plant growth-promoting mechanisms and potential applications for agricultural sustainability. Antonie van Leeuwenhoek. 113: 1075-1107. doi: 10.1007/ s10482-020-01429-y
  • Rezaei-Chiyaneh E, Amirnia R, Machiani MA, Javanmard A, Maggi F et al. (2020). Intercropping fennel (Foeniculum vulgare L.) with common bean (Phaseolus vulgaris L.) as affected by PGPR inoculation: A strategy for improving yield, essential oil and fatty acid composition. Scientia Horticulturae 261: 1-11. doi: 10.1016/j.scienta.2019.108951
  • Sahu PK, Singh S, Gupta AR, Gupta A, Singh UB (2020). Endophytic bacilli from medicinal-aromatic perennial holy basil (Ocimum tenuiflorum L.) modulate plant growth promotion and induced systemic resistance against Rhizoctonia solani in rice (Oryza sativa L.). Biological Control 150: 104353. doi: 10.1016/j.biocontrol.2020.104353
  • Salam N, Khieu TN, Liu MJ, Vu TT, Chu-Ky S et al. (2017). Endophytic actinobacteria associated with Dracaena cochinchinensis Lour.: Isolation, diversity, and their cytotoxic activities. BioMed Research International 1308563: 1-17. doi: 10.1155/2017/1308563.
  • Sahin U, Anapali O, Ercisli S (2002). Physico-chemical and physical properties of some substrates used in horticulture. Gartenbauwissenschaft 67:55-60.
  • Salehi B, Stojanovic-Radic Z, Matejic H, Sharopov F, Antolak H et al. (2018). Plants of genus Mentha: From farm to food factory. Plants 7: 1-36. doi: 10.3390/plants7030070
  • Santana P, Busato de Feiria SN, Höfling JS (2016). Medicinal plants as a source of new therapeutic products: Genus Mentha and the potential antimicrobial activity of extracts and essential oils. Current Traditional Medicine 2: 94-114. doi: 10.2174/22 15083802666161104154842
  • Singh A, Gupta R, Saikia SK, Pant A, Pandey R (2016). Diseases of medicinal and aromatic plants, their biological impact and management. Plant Genetic Resources 14: 370-383. doi: 10.1017/S1479262116000307
  • Singh M, Kumar A, Singh R, Pandey KD (2017). Endophytic bacteria: a new source of bioactive compounds. 3 Biotech 7: 1-14. doi: 10.1007/s13205-017-0942-z
  • Singh P, Pandey AK (2018). Prospective of essential oils of the genus Mentha as biopesticides:A review. Frontiers in Plant Science 9: 1-14. doi: 10.3389/fpls.2018.01295
  • Snoussi M, Noumi E, Trabelsi N, Flamini G, Papetti A et al. (2015). Mentha spicata essential oil: Chemical composition, antioxidant and antibacterial activities against planktonic and biofilm cultures of Vibrio spp. strains. Molecules 20: 14402-14424. doi: 10.3390/molecules200814402.
  • Subasi I (2020). Seed fatty acid compositions and chemotaxonomy of wild Crambe (Brassicaceae) taxa in Turkey. Turkish Journal of Agriculture and Forestry 44:662-670.
  • Tamilarasi S, Nanthakumar K, Karthikeyan K, Lakshmanaperumalsamy P (2008). Diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of Coriandrum sativum. Journal of Environmental Biology 29: 127–134.
  • Toussaint JP, Kraml M, Nell M, Smith SE, Smith FA (2008). Effect of Glomus mosseae on concentrations of rosmarinic and caffeic acids and essential oil compounds in basil inoculated with Fusarium oxysporum f. sp. basilici. Plant Pathology 57: 1109– 1116. doi: 10.1111/j.1365-3059.2008.01895.x
  • Vaikuntapu PR, Dutta S, Samudrala RB, Rao VRVN, Kalam S (2014) Preferential promotion of Lycopersicon esculentum (tomato) growth by plant growth promoting bacteria associated with tomato. Indian Journal of Microbiology 54: 403–412. doi: 10.1007/s12088-014-0470-z
  • Vendan RT, Yu YJ, Lee SH, Rhee YH (2010). Diversity of endophytic bacteria in ginseng and their potential for plant growth promotion. The Journal of Microbiology 48: 559–565. doi: 10.1007/ s12275-010-0082-1
  • Vining KJ, Zhang AO, Tucker CS, Davis TM (2005). Mentha longifolia (L.) L.: A model species for mint genetic research. American Society for Horticular Science 40: 1225-1229. doi: 10.21273/ hortsci.40.5.1225
  • Vyawahare MA, Jagta PR, Gangurde AB, Kukreja GP, Mane RS (2019). Isolation of endophytes from roots of Aloe Vera. IJSART 5: 273-276.
  • Wilson K (1997). Preparation of genomic DNA from bacteria. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smit JA, Struhl K (editors). Current protocols in molecular biology. Newyork, USA: Wiley, pp. 241-245.
  • Zhao LX, Xu LH, Jiang CH (2012). Methods for the study of endophytic microorganisms from traditional Chinese medicine plants. In: Zhao LX, Xu LH (editors). Methods in Enzymology. Boston, USA: Academic Press, pp. 3-21.
  • Zia-Ul-Haq M, Ahmad S, Qayum M, Ercisli S (2013). Compositional studies and antioxidant potential of Albizia lebbeck (L.) Benth. Pods and seeds. Turkish Journal of Biology 37 (1): 25-32.
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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