Genetic variation and potential genetic resources of several Romanian larch populations
Genetic variation and potential genetic resources of several Romanian larch populations
RAPD molecular markers and phenotypic growth traits were joined using different genotypes of Larix decidua (Mill.) toanalyze genetic variation. Individual samples of vegetative clones were analyzed from Baciu orchard (obtained through a breedingprogram from regional stands from Romania) and pooled samples from places of origin (regional stands). Based on coefficients ofvariation for tree height, diameter, and volume, the greatest genetic variation in the orchard was observed at the Gura Humorului andAnina provenances (24.4%, 27.5%, and 14.4% for Gura Humorului and 26.9%, 29.46%, and 15.5% for Anina). In addition, the treeswhose origins are Gura Humorului and Anina form the thickest branches. In stands from origin areas, the best performances of growthtrait in height were scored in the Valea Popii (33.5 m) and Anina (33.1 m) populations. Stands from Valea Cetatii and Sacele recordedthe smallest diameters. Both in orchards and in regional stands the trees with the greatest vigor were those from Gura Humorului andAnina and large variability was noted, which allows efficient selection in order to identify superior trees useful for larch breeding. RAPDanalysis illustrated the genetic differences among the larch proveniences and some provenances represented distinct genetic entities,recognizable at the molecular level. The dendrogram indicates the diversity of the genotypes divided into seven groups as were thecollection sites. Polled samples of DNA proved to be a valuable tool for determining the phylogenetic relationships in larch provenances.Thus, most populations from regional stands are grouped in the same branch (group) with clones from orchard. The correlation betweengenetic distance and geographical distance was not significant, with a correlation coefficient of 0.035. In addition, a slight decrease wasobserved in genetic distance with increase in geographical distance between genotypes.
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- Abdulla M, Gamal O (2010). Investigation on molecular phylogeny
of some date palm (Phoenix dactylifra L.) cultivars by protein,
RAPD and ISSR markers in Saudi Arabia. Aust J Crop Sci 4:
23-28.
- Arcade A, Faivre-Rampant P, Le Guerroué B, Pâques LE, Prat D
(1996). Heterozygosity and hybrid performance in larch. Theor
Appl Genet 93: 1274-1281.
- Biswas C, Mohri BM (1997). The Gymnosperms. Berlin, Germany:
Springer.
Bruchwald A, Zasada M (2010). Growth model for European larch
(Larix decidua Mill.). Sylwan 154: 615-624.
- Bucci G, Vendramin GG, Lelli L, Vicario F (1997). Assessing the
genetic divergence of Pinus leucodermis Ant. endangered
populations: use of molecular markers for conservation
purposes. Theor Appl Genet 95: 1138-1146.
- Carter K, Adams K, Greenwood GW, Nitschkep MS (1990). Early
family selection in jack pine. Can J For Res 20: 285-291.
- Chaw SM, Zharkikh A, Sung HM, Leu TC, Li WH (1997). Molecular
phylogeny of extant gymnosperms and seed plant evolution:
analysis of nuclear 18S rRNA sequences. Mol Biol Evol 14: 56-
68.
- Chuine I, Aitken S, Ying C (2001). Temperature thresholds of shoot
elongation in provenances of Pinus contorta. Can J For Res 31:
1444-1455.
- Cleland EE, Chuine I, Menzel A, Mooney HA, Schwartz MD (2007).
Shifting plant phenology in response to global change. Trends
in Ecology and Evolution 22: 357-365.
- Curtu AL, Sofletea N, Radu R, Bacea A, Abrudan IV, Butiuc-Keul A,
Farcas S (2009). Allozyme variation of coniferous tree species
from Maramures mountains, Romania. Not Bot Horti Agrobo
37: 245-251.
- Deng JF, Zhang HG, Zhang L, Guan CY, Zhang L (2010). Genetic
variation of 17-year-old hybrid larch and its superior family
selection. J North For Univ 38: 8-11.
- Farmer RE, O’Reilly G, Shaotang D (1993). Genetic variation in
juvenile growth of tamarack (Larix laricina) in Northwestern
Ontario. Can J For Res 23: 1852-1862.
- Gierlinger N, Jacques D, Grabner M, Wimmer R, Schwanninger M,
Rozenberg P, Pâques LE (2004). Colour of larch heartwood and
relationships to extractives and brown-rot decay resistance.
Trees 18: 102-108.
- Goto S, Thakur RC, Ishii K (1998). Determination of genetic stability
in long-term micropropagated shoots of Pinus thunbergii Parl.
using RAPD markers. Plant Cell Reports 18: 193-197.
- Gugerli F, Sperisen C, Büchler U, Magni F, Geburek T, Jeandroz S,
Senn J (2001). Haplotype variation in a mitochondrial tandem
repeat of Norway spruce (Picea abies) populations suggests a
serious founder effect during postglacial re-colonization of the
western Alps. Mol Ecol 10: 1255-1263.
- Hampl V, Pavlicek A, Flegr J (2001). Construction and bootstrap
analysis of DNA fingerprinting-based phylogenetic trees with
the freeware program FreeTree: application to Trichomonad
parasites. Int J Syst Evol Microbiol 51: 731-735.
- Hänninen H (1997). Effects of climate change on overwintering of
forest trees in temperate and boreal zones. A review of the
frost damage hypothesis. In: Mohren GMJ, Kramer K, Sabaté
S, editors. Impacts of Global Change on Tree Physiology and
Forest Ecosystems. Dordrecht, Netherlands: Kluwer Academic
Press, pp. 149-158.
- Huang S, Wang BJ, Lu J, Dai C, Lei Y, Sun X (2012). Characterizing
Changbai Larch through veneering. Part 1: effect of stand
density. BioResorces 7: 2444-2460.
- Kang H (1991). Recurrent selection, mating design, and effective
population size. In: Proceedings 21st Southern Forest Tree
Improvement Conference June 1991, Knoxville, TN, USA, pp.
129-143.
- Khatab IA, Ishiyama H, Inomata N, Wang XR, Szmidt AE (2008).
Phylogeography of Eurasian Larix species inferred from
nucleotide variation in two nuclear genes. Genes Genet Syst
83: 55-66.
- Kongkiatngam P, Waterway MJ, Coulman BE, Fortin MG (1996).
Genetic variation among cultivars of red clover (Trifolium
pratense L.) detected by RAPD markers amplified from bulk
genomic DNA. Euphytica 89: 355-361.
- Kozyrenko MM, Artyukova EV, Reunova GD, Levina EA, Zhuravlev
Yu N (2004). Genetic diversity and relationships among
Siberian and far Eastern larches inferred from RAPD analysis.
Russ J Genet 40: 401-409.
- Kraft T, Säll T (1999). An evaluation of the use of pooled samples in
studies of genetic variation. Heredity 82: 488-494.
- Kramer K (1994). Selecting a model to predict the onset of growth of
Fagus sylvatica. J Appl Ecol 31: 172-181.
- Kramer K (1995a). Modelling comparison to evaluate the importance
of phenology for the effects of climate change on growth of
temperate-zone deciduous trees. Clim Res 5: 119-130.
- Kramer K (1995b). Phenotypic plasticity of the phenology of seven
European tree species in relation to climatic warming. Plant,
Cell Environ 18: 93-104.
- Kramer K, Leinonen I, Loustau D (2000). The importance of
phenology for the evaluation of impact of climate change
on growth of boreal, temperate and Mediterranean forests
ecosystems: an overview. Int J Biometeorol 44: 67-75.
- Larionova AI, Iakhneva NV, Abaimov AP (2004) Genetic diversity
and differentiation of Gmelin larch Larix gmelinii populations
from Evenkia (Central Siberia). Genetika 40: 1370-1377.
- Lee SW, Ledig FT, Johnson DR (2002). Genetic variation at allozyme
and RAPD markers in Pinus longaeva (Pinaceae) of the White
Mountains, California. Am J Bot 89: 566-577.
- Leng WF, He HS, Bu RC, Dai LM, Hu YM, Wang XG (2008).
Predicting the distributions of suitable habitat for three larch
species under climate warming in Northeastern China. Forest
Ecol Manag 254: 420-428.
- Levina EA, Adrianova IYu, Reunova GD, Zhuravlev YuN (2008).
Genetic variability and differentiation in the larch populations
within the range of Larix olgensis A. Henry in Primorye. Russ
J Genet 44: 320-325.
- Lewandowski A, Mejnartowicz L (1991). Levels and patterns of
allozyme variation in some European larch Larix-decidua
populations. Hereditas 115: 221-226.
- Li B, Williams CG, Carlson WC, Harrington CA, Lambeth C (1992).
Gain efficiency in short-term testing: experimental results. Can
J For Res 22: 290-297.
- Li B, Wyckoff GW (1994). Breeding strategies for Larix decidua,
L. leptolepis and their hybrids in the United States. Forest
Genetics 1: 65-72.
- Li SG, Tsujimura M, Sugimoto A, Davaa G, Oyunbaatar D, Sugita
M (2007). Temporal variation of d13C of larch leaves from a
montane boreal forest in Mongolia. Trees 21: 479-490.
- Lodhi MA, Ye GN, Weeden NF, Reisch BI (1994). A simple and
efficient method DNA extraction from grapevine cultivars and
Vitis species. Plant Mol Biol Reptr 12: 6-13.
- Lukkarinen AJ, Ruotsalainen S, Nikkanen T, Peltola H (2010).
Survival, height growth and damages of Siberian (Larix sibirica
Ledeb.) and Dahurian (Larix gmelinii Rupr.) larch provenances
in field trials located in Southern and Northern Finland. Silva
Fennica 44: 727-747.
- Mantel N (1967). The detection of disease clustering and a generalized
regression approach. Cancer Res 27: 209-220.
- McComb AL (1955). The European larch: its races, site requirements,
and characteristics. Forest Science 1: 298-318.
- Mihai G, Teodosiu M (2009). Genetic diversity and breeding of larch
(Larix decidua Mill.) in Romania. Ann For Res 52: 97-108.
- Nagaike T, Hayashi A, Kubo M (2010). Diversity of naturally
regenerating tree species in the overstorey layer of Larix
kaempferi plantations and abandoned broadleaf coppice stands
in central Japan. Forestry 83: 285-291.
- Nawrot M, Pazdrowski W, Szymanski M, Kamierczak K (2009).
Wood quality of butt logs in European larch (Larix decidua
Mill.) and diameter increment dynamics. Ann WULS-SGGW,
For and Wood Technol 69: 104-108.
- Oreshkova NV, Belokon MM, Jamiyansuren S (2013). Genetic
diversity, population structure, and differentiation of Siberian
larch, Gmelin larch, and Cajander larch on SSR-marker data.
Russ J Genet 49: 178-186.
- Oreshkova NV, Larionova AY, Milyutin LI, Abaimov AP (2006).
Genetic diversity structure and differentiation of Gmelin
Larch (Larix gmelinii (Rupr.) Rupr.) populations from Central
Evenkia and Eastern Zabaikalje. Eurasian J For Res 9: 1-8.
- Paques LE, Millier F, Rozenberg P (2010). Selection perspectives for
genetic improvement of wood stiffness in hybrid larch (Larix x
eurolepis Henry). Tree Genet Genomes 6: 83-92.
- Paques LE, Sylvestre-Guinot G, Delatour C (1999). Genetic variation
among clones of Larix decidua Polonica for resistance to
Lachnellula willkommii. Ann For Sci 56: 155-166.
- Pazdrowski W, Jelonek T, Tomczak A, Stypula I, Splawa-Neyman S
(2007). Proportion of sapwood and heartwood and selected
biometric features in larch trees (Larix decidua Mill). Wood
Research 52: 1-16.
- Persson B, Beuker E (1997). Distinguishing between effects of
changes in temperature and light climate using provenance
trial with Pinus sylvestris in Sweden. Can J For Res 27: 572-579.
- Plomion C, Leprovost G, Stokes A (2001). Wood formation in trees.
Plant Physiol 127: 1513-1523.
- Price R (1993). Familial relationships of the conifers from rbcL
sequence data. Am J Bot 80: 172.
- Reinhardt E (1983). Using height/diameter curves to estimate
site index in old-growth Western larch stands. Res. Note 20,
Montana Forest and Conservation Exp. Stn., School of For.,
Univ of Montana, pp 3.
- San Jose-Maldia L, Uchida K, Tomaru N (2008). Mitochondrial
DNA variation in natural populations of Japanese larch (Larix
kaempferi). Silv Genet 58: 234-241.
- Scheepers D, Eloy MC, Briquet M (1999). Identification of larch
species (Larix decidua, Larix kaempferi and Larix X eurolepis)
and estimation of hybrid fraction in seed lots by RAPD
fingerprints. Theor Appl Genet 100: 71-74.
- Schlötterer C, Tobler R, Kofler R, Nolte V (2014). Sequencing pools
of individuals - mining genome-wide polymorphism data
without big funding. Nat Rev Genet 15: 749-763.
- Shahraji TR, Kazempour MN, Adbesh Z (2009). Genetic (RAPD)
diversity in loblolly pine unknown provenance plantation in
Iran. Indian J Hortic 66: 35-38.
- Shearer RC (2008) Larix Mill., In: Bonner FT, Karrfalt RP editors.
The Woody Plant Seed manual Agric. Handbook no 727.
Washington DC, USA, pp. 637-650.
- Stefanovic S, Jager M, Deutsch J, Broutin J, Masselot M (1998).
Phylogenetic relationships of conifers inferred from partial 28S
rRNA gene sequences. Am J Bot 85: 688-697.
- Sweeney PM, Danneberger TK (1994). Random amplified
polymorphic DNA in perennial ryegrass: a comparison of bulk
samples vs. individuals. Hort Science 29: 624-626.
- Vanclay JK (1992). Sustainable timber harvesting: simulation studies
in the tropical rainforests of north Queensland. For Ecol
Manag 69: 299-320.
- Wang MB, Gao FQ (2009). Genetic variation in Chinese pine (Pinus
tabulaeformis), a woody species endemic to China. Biochem
Genet 47: 154-164.
- Wang S, Wen J, Yang Z, Ma F (2006). Effects site conditions on
industrial fiber plantations of Larix olgensis. J Forest Res 17:
255-258.
- Xu Z (1992). Fruiting of Larix gmelinii in 1989 in Tuqiang Forestry
Bureau jurisdiction area of the Greater Xingan Mountains.
Chinese Journal of Ecology 11: 8-12.
- Yu P, Pauls KP (1993). Rapid estimation of genetic relatedness among
heterogeneous populations of alfalfa by random amplification
of pooled genomic DNA samples. Theor Appl Genet 86: 788-
794.
- Zhang L, Zhang HG, Li XF (2013). Analysis of genetic diversity in
Larix gmelinii (Pinaceae) with RAPD and ISSR markers. Genet
Mol Res 12: 196-207.