Effects of NaCl on the Germination, Seedling Growth and Water Uptake of Triticale

The lack of information about the effects of salinity on vegetative growth of triticale prompted us to study the salt tolerance levels of 3 newly registered cultivars, Karma-2000, Presto, and Tatlıcak-97. Moreover, the relative importance of the osmotic or toxic effects of NaCl on seed germination are not clear in triticale. In this study, germination percentage, seedling fresh and dry weight and water uptake of the seeds of 3 triticale cultivars were determined under various salt (NaCl) stresses with electrical conductivities of 2.4, 4.2, 5.9, 7.7, 10.6 and 13.2 dS m-1. Roots and shoots were analyzed for uptake of Na+ and K+, and seeds for Na+, K+ and Cl-. The results showed that increased NaCl significantly affected mean germination time without affecting final germination percentage. In each salt concentration, water uptake was not significantly changed. Salinity increased the accumulation of Na+ and decreased the K+ content in the roots and shoots. Moreover, the Na+ and Cl- content of germinating seeds gradually increased, while K+ diminished. Karma-2000 appeared to be more tolerant to salt stress than the others. It was concluded that the delay in germination was mainly due to higher Na+ accumulation in the seeds rather than osmotic stress in triticale cultivars, while final germination percentage was not changed by NaCl. However, it appeared that NaCl adversely influenced triticale seedling characters.

Effects of NaCl on the Germination, Seedling Growth and Water Uptake of Triticale

The lack of information about the effects of salinity on vegetative growth of triticale prompted us to study the salt tolerance levels of 3 newly registered cultivars, Karma-2000, Presto, and Tatlıcak-97. Moreover, the relative importance of the osmotic or toxic effects of NaCl on seed germination are not clear in triticale. In this study, germination percentage, seedling fresh and dry weight and water uptake of the seeds of 3 triticale cultivars were determined under various salt (NaCl) stresses with electrical conductivities of 2.4, 4.2, 5.9, 7.7, 10.6 and 13.2 dS m-1. Roots and shoots were analyzed for uptake of Na+ and K+, and seeds for Na+, K+ and Cl-. The results showed that increased NaCl significantly affected mean germination time without affecting final germination percentage. In each salt concentration, water uptake was not significantly changed. Salinity increased the accumulation of Na+ and decreased the K+ content in the roots and shoots. Moreover, the Na+ and Cl- content of germinating seeds gradually increased, while K+ diminished. Karma-2000 appeared to be more tolerant to salt stress than the others. It was concluded that the delay in germination was mainly due to higher Na+ accumulation in the seeds rather than osmotic stress in triticale cultivars, while final germination percentage was not changed by NaCl. However, it appeared that NaCl adversely influenced triticale seedling characters.

___

  • Begum, F., J.L. Karmoker, Q.A. Fattah and A.F.M. Maniruzzaman. 1992. The effect of salinity on germination and its correlation with K, Na, Cl accumulation in germinating seeds of Triticum aestivum cv. Akbar. Plant Cell Physiol., 33: 1009-1014.
  • Bernstein, L. 1975. Effect of salinity and sodicity on plant growth. Ann. Rev. Phytopathol. 13: 295-312.
  • Bewley, J.D. and M. Black. 1982. Physiology and Biochemistry of Seeds in Relation to Germination, Vol. 2. Springer- Verlag, Berlin.
  • Böhm, W. 1979. Methods of Studying Root Systems, Springler-Verlag, Berlin.
  • Bray, J.R. 1963. Root production and the estimation of net productivity. Can. J. Bot. 41: 65-72.
  • Briggle, L. W. 1969. Triticale- A Review. Crop Science, 9: 197-200.
  • Coons, J. M., R.O. Kuehl and N.R. Simons. 1990. Tolerance of ten lettuce cultivars to high temperature combined with NaCl during germination. J. Amer. Soc. Hort. Sci. 115: 1004-1007.
  • Craig, G.F., D.T. Bell and C.A. Atkins 1990. Responses to salt stress and waterlodgings stress ten taxa of Acacia selected from naturally saline areas of Western Austrailia. Aust. J. Bot., 38: 619-630.
  • Demir, I., K. Mavi, M. Özcoban and G. Okcu. 2003. Effect of salt stress on germination and seedling growth in serially harvested aubergine (Solanum melongena L.) seeds during development. Israel J. Plant Sci., 51: 125-131.
  • Ellis, R.H. and E.H. Roberts. 1980. Towards a rational basis for seed testing seed quality. In: Hebblethwaitei P., ed. Seed Production. Butterworths, London, pp. 605-635.
  • Feng Ma, J., S. Taketa and A.Z. Ming Ya. 2000. Aluminium tolerance genes on the short arm of chromosome 3R are linked to organic acid release in triticale. Plant Physiol., 122: 687-694.
  • Francois, L.E., E.V. Maas, T.J. Donovani and V.L. Youngs. 1986. Effect of salinity on grain yield and quality, vegetative growth, and germination of semi-dwarf and durum wheat. Agronomy Journal, 78: 1053-1058.
  • Francois, L.E., T.J. Donovan, E.V. Maas and G.L. Rubernthaler. 1988. Effect of salinity on grain yield and quality, vegetative growth and germination of triticale. Agron. J., 80: 642-647.
  • Frota, J.N.E. and T.C. Tucker. 1978. Absorption rates of ammonium and nitrate by red kidney beans under salt and water stress. Soil Sci. Soc. Am. J. 42: 753-757.
  • Ghorashy, S.R., N. Sionit and M. Kheradnam. 1972. Salt tolerance of safflower varieties (Carthamus tinctorius L.) during emergence. Agron. J., 64: 256.
  • Ghoulam, C. and K. Fares. 2001. Effect of salinity on seed germination and early seedling growth of sugar beat (Beta vulgaris L.). Seed Sci. Technol. 29: 357-364.
  • Gupta, S.C. and C.P. Srivastava. 1989. Effect of salt stress on morpho- physiologiocal parameters in wheat (Triticum aestivum L.). Indian J. Plant Physiol. XXXII:2, 169-171.
  • Hampson, C.R. and G.M. Simpson. 1990. Effect of temperature, salt and osmotic potential on early growth of wheat (Triticum aestivum L.). I. Germination. Can. J. Bot., 68: 524-528.
  • ISTA, 1996. International rules for seed testing. Rules. Seed Sci. Technol. 24. Supplement.
  • Johnson, C.M. and A. Ulrich. 1959. II. Analytical methods for use in plant analysis. California Agricultural Experiment Station. Bull. 766.
  • Jones, R.A. 1986. High potential in Lycopersicum species during germination. Euphytica. 35: 575-582.
  • Karim, M.A., E. Nawata and S. Shingenaga. 1993. Salinity tolerance of hexaploid triticale cultivars at different growth stages. Japanese Journal of Tropical Agriculture, 37: 298-304.
  • Khajeh-Hosseini, M., A.A. Powell and I.J. Bingham. 2003. The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed Sci. & Technol., 31: 715- 725.
  • Leopold, A.C. and R.P. Willing. 1984. Evidence of toxicity effects of salt on membranes. In: Salinity Tolerance in Plants, (eds. R.C. Staples and G.H. Toenniessen), pp. 67-76. John Wiley and Sons, New York.
  • Mansour, M.M.F. 1994. Changes in growth, osmotic potential and cell permeability of wheat cultivars under salt stress. Biologia Plantarum, 36: 429-434.
  • Mass, E.V. and G.J. Hoffman. 1977. Crop salt tolerance-current assessment. J. Irrig. Drain Div. 103: 115-134.
  • Meiri, A. 1984. Plant response to salinity: Experimental methodology and application to the field. Soil salinity under irrigation processes and management. Springer-Verlag, Berlin.
  • Perez-Alfocea, F., M.T. Estan, M. Caro and M.C. Bolarin. 1993. Responses of tomato cultivars to salinity. Plant and Soil. 69: 25- 31.
  • Pesarrakli, M. 1999. Handbook of Plant and Crop Stress. Marcel Decker Inc. NewYork.
  • Pesserakli, M and T.C. Tucker. 1988. Dry matter yield and nitrogen–15 uptake by tomatoes under sodium chloride stress. Soil Sci. Soc. Am. J., 52: 768-700.
  • Pesserakli, M. and T.C. Tucker. 1985. Uptake of nitrogen-15 by cotton under salt stress. Soil Sci. Soc. Am. J., 49: 149-152.
  • Pesserakli, M., T.C. Tucker and K. Nakabayaski. 1991. Growth response of barley and wheat to salt stress. Journal of Plant Nutrition, 14: 331-340.
  • Rehman, S., P.J.C. Harris, W.F. Bourne and J. Wilkin. 1996. The effect of sodium chloride on germination and the potassium and calcium content of Acacies seeds. Seed Sci. Technol., 25: 45-57.
  • Rhoades, J.D., A. Kandiah and A.M. Mashali. 1992. The Use of Saline Waters for Crop Production. FAO Irr. And Drain. Paper no: 48, p:1-133, Rome.
  • Salim, M. 1991. Comparative growth responses and ionic relations of four cereals during salt stress. J. Agronomy and Crop Science, 166: 204-209.
  • Van Hoorn, J.W. 1991. Development of soil salinity during germination and early seedling growth and its effect on several crops. Agricultural Water Management, 20: 17-28.