Differences of flavonoid structural genes preferentially expressed in brown and green natural colored cotton
Differences of flavonoid structural genes preferentially expressed in brown and green natural colored cotton
We compared the expression levels of some structural genes involved in the flavonoid pathway between two brown cotton lines (brown 16 and light brown 14) and two green cotton lines (greenish 12 and light green 5). Gene expression levels of six structural genes F3’h (flavonoid 3ˈ-hydroxylase), F3’5’h (flavonoid 3ˈ5ˈ-hydroxylase), Dfr (dihydroflavonol 4-reductase), Lar (leucoanthocyanidin reductase), Ans (anthocyanidin synthase), and Anr (anthocyanidin reductase) were all substantially highly expressed in both brown cotton lines than in green cotton lines. Our study also revealed differences in expression levels between the two brown cotton lines. F3’h and F3’5’h had higher expression in the brown than in the light brown fibers, suggesting that increasing expression of these genes resulted in more of the proanthocyanidin pigments that give color to the brown lines. None of the genes examined were differentially expressed in the two green lines, suggesting the color difference is not due to products of the flavonoid pathway. The results suggest that breeding efforts to introduce brown colors into white-fiber lines with high-quality fiber focus on introgressing F3’h and F3’5’h and that naturally occurring allelic variants affecting the expression levels of these genes could be used to control the intensity of brown pigmentation
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- Balasubramanian VK, Rai KM, Thu SW, Hii MM, Mendu V (2016). Genome-wide identification of multifunctional laccase gene family in cotton (Gossypium spp.); expression and biochemical analysis during fiber development. Scientific Reports. 6: 34309. doi: 10.1038/srep34309
- Bland JM, Altman DG (1995). Multiple significance tests: the Bonferroni method. BMJ 310 (36986): 710. doi: 10.1136/ bmj.310.6973.170
- Feng HJ, Sun JL, Wang J, Jia YH, Zhang XY et al. (2011). Genetic effects and heterosis of the fiber color and quality of brown cotton (Gossypium hirsutum). Plant Breeding 130: 450-456. doi: 10.1111/j.1439-0523.2010.01842.x
- Feng H, Tian X, Liu Y, Li Y, Zhang X et al. (2013). Analysis of flavonoids structural genes in Brown fiber of upland cotton. PLoS ONE 8: 1-10. doi: 10.1371/journal.pone.0058820
- Feng H, Guo L, Wang G, Sun J, Pan Z et al. (2015). The negative correlation between fiber color and quality traits revealed by QTL analysis. PLoS ONE 10 (6): e0129490. doi: 10.1371/ journal.pone.0129490
- Gong W, He S, Tian J, Sun J, Pan Z et al. (2014). Comparison of the transcriptome between two cotton lines of different fiber color and quality. PLoS ONE 9 (11): e112966. doi: 10.1371/journal. pone.0112966
- Hua SJ, Wang XD, Yuan SN, Shao MY, Zhao XQ et al. (2007). Characterization of pigmentation and cellulose synthesis in colored cotton fibers. Crop Science 47: 1540-1546. doi: 10.2135/cropsci2006.12.0835
- Li XB, Fan XP, Wang XL, Cai L, Yang WC (2005). The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation. Plant Cell 17: 859-875. doi: 10.1105/tpc.104.029629
- Li Y-J, Zhang X-Y, Wang F-X, Yang C-L, Liu F et al. (2013). A comparative proteomic analysis provides insights into pigment biosynthesis in brown color fiber. Journal of Proteome 78: 374- 388. doi: 10.1016/j.jprot.2012.10.005
- Liu HF, Luo C, Song W, Shen, Li G, He ZG et al. (2018). Flavonoid biosynthesis controls fiber color in naturally colored cotton. PeerJ 6: e4537. doi: 10.7717/peerj.4537
- Mathesius U, Schlaman HR, Spaink HP, Sautter C, Rolfe BG et al. (1998). Auxin transport inhibition precedes root nodule formation in white clover rootsand cis regulated by flavonoids and derivatives of chitin oligosaccharides. Plant Journal 14: 23- 34. doi: 10.1046/j.1365-313X.1998.00090.x
- Murphy A, Peer WA, Taiz L (2000). Regulation of auxin transport by aminopeptidases and endogenous flavonoids. Planta 211: 315- 324. doi: 10.1007/s004250000300
- Peer WA, Bandyopadhyay A, Blakeslee JJ, Makam SN, Chen RJ et al. (2004). Variation in expression and protein localization of the PIN family of auxin efflux facilitator proteins in flavonoid mutants with altered auxin transport in Arabidopsis thaliana. Plant Cell 16: 1898-1911. doi: 10.1105/tpc.021501
- Petrussa E, Braidot E, Zancani M, Peresson C, Bertolini A et al. (2013). Plant flavonoids biosynthesis, transport and involvement in stress responses. International Journal of Molecular Science 14: 14950-14973. doi: 10.3390/ijms140714950
- Tan J, Tu L, Deng F, Hu H, Nie Y et al. (2013). A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin1[W][OA]. Plant Physiology 162: 86-95. doi: 10.1104/pp.112.212142
- Yuan S, Hua S, Malik W, Bibi N, Wang X (2012). Physiological and biochemical dissection of fiber development in colored cotton. Euphytica 187: 215-226. doi: 10.1007/s10681-012-0653-9
- Zhang J, Gong Z, Sun J, Liu J (1994). Heterosis of yield and fiber performance in interspecific crosses between Gossypium hirsutum and G. barbadense. Acta Gossypii Sinica 6: 140-145.
- Wang L, Liu H, Li X, Xiao X, Ai X et al. (2014). Genetic mapping of fiber color genes on two brown cotton cultivars in Xinjiang. SpringerPlus 3: 480. doi: 10.1186/2193-1801-3-480
- Xiao YH, Zhang ZS, Yin MH, Luo M, Li XB et al. (2007). Cotton flavonoid structural genes related to the pigmentation in brown fibers. Biochemical Biophysical Research Communications 358: 73-78. doi: 10.1016/j.bbrc.2007.04.084
- Xiao Y-H, Yan Q, Ding H, Luo M, Hou L et al. (2014). Transcriptome and biochemical analyses revealed a detailed proanthocyanidin biosynthesis pathway in brown cotton fiber. PLoS ONE 9 (1): doi: 10.1371/journal.pone.0086344