Ceviz (Juglans regia L.) somatik embriyolarının doymuş tuz solusyonları ile kurutulması ve embriyolarda çimlenme oranının artırılması

Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different dura-tions; relationships between desiccation and germination or root formation in the dark; and effects of desiccation and gibberellic acid on germinating of 'Su-2' walnut somatic embryonic embryo line were investigated. Survival of embryos was low (about 10%) or zero when loss of fresh weight was about 90% and dry matter was about 70%. The highest germinating percentages (13.3-23.3%) were obtained with about 40.4-77.3% of loss of fresh weight and 9.1-34.6% of dry matter of desiccated embryos in covered plates over saturated $MgCl_2$ .$6_2O$ for 4 days, $Mg(NO_3)_2$ .$6H_2O$ for 5 days or NaCl for 5-6 days, and with uncovered plates over $ZnSO_4$ .$7H_2 O$ for 6-7 days or $NH_3 SO_4$ for 6-8 days. The highest rooting percentage (80.9%) in the dark were obtained with 56.2% of loss of fresh weight and 16.8% of dry matter of desiccated embryos in covered plates over saturated $MgCl_2$ . $6H_ 2O$ for 4 days. To improve germination, both desiccation and GA 3 treatments were more effective than only desiccation or GA 3 treatments on germination. Germinating was best (46.0%) with desiccation followed by incubation in the dark for 15 days prior to being transferred to a medium containing 9 mg/l GA 3 in the light.

Dessiccation using saturated salt solutions and imprrovement germination rate of walnut (Juglans regia L.) somatic embryos

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